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Citations for R&D Systems :
301 - 350 of 440 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... FL5.12 cells were cultured in the presence of 10 ng/ml IL-3 (R&D Systems). Cell cycle exit in FL5.12 was achieved by washing the cells three times with PBS to remove IL-3 from the cells and by placing the cells in IL-3 free culture media for 48 h ...
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bioRxiv - Neuroscience 2024Quote: ... coated plates in the expansion medium (N2B27) supplemented with 3 µM CHIR (R&D System, 4423), 0.5 µM PMA (Cayman Chemical ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL mouse IL-3 and 10 ng/mL mouse IL-6 (R&D Systems). The efficiency of Gata2 gene editing was assessed by ICE CRISPR Analysis Tool (https://ice.synthego.com/) ...
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bioRxiv - Molecular Biology 2020Quote: ... SH-SY5Y and SK-N-AS cells were pre-treated for 16 h with 1000 Units/ml Interferon Type 1 (Interferon α/β, R&D Systems (Minneapolis, MN, USA)) followed by 2,5 μM BV6 (Selleckchem ...
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bioRxiv - Immunology 2021Quote: ... Sanofi-aventis Cat# NAC2004–5843-01) and interleukin-4 (IL-4; 1000 IU/mL; R&D Systems Cat# 204-1 L). Mature ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl 1.2% barium chloride (w/v in H2O) with or without 2 µg of Osteopontin neutralizing antibody (AF808, R&D Systems) was injected into a TA muscle ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The D1-HEK cells were transfected overnight and the following morning split into poly-L-lysine (R&D systems, Minneapolis, MN) coated 96-well white clear bottom cell culture plates (Greiner Bio-One ...
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bioRxiv - Developmental Biology 2020Quote: ... or Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3) was placed in the upper and lower chambers ...
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bioRxiv - Microbiology 2021Quote: ... organoids were washed twice with PBS and resuspended in Cultrex 3-D Culture Matrix (R&D Systems) for 30 min before organoid medium (as described above ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was quantified using Human Dkk-3 DuoSet Solid Phase Sandwich ELISA (DY1118, R&D Systems, MN), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems), 200 ng/mL FGF9 (fibroblast growth factor 9 ...
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bioRxiv - Cancer Biology 2021Quote: We cultured 3*105 million freshly enriched DCs in 2ml Dendritic Cell Base Media (R&D systems) plus 10% FBS ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used were rabbit anti-Human Phospho-Smad2/3 (R&D Systems MAB8935, 1:500 dilution), goat anti-histone (Abcam ab12079 ...
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bioRxiv - Bioengineering 2024Quote: ... which was supplemented with 3 ng/mL human activin A (R&D Systems, Inc., Minneapolis, MN, USA), 1 ng/mL human bone morphogenetic protein 4 (Bmp4 ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 mM HMGB1(R&D systems) diluted with protein block buffer (Dako ...
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bioRxiv - Immunology 2023Quote: ... or with 10 µl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693) ...
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bioRxiv - Cell Biology 2023Quote: Immunohistochemistry was performed with the following antibodies: Cleaved Caspase 3 (rabbit, R&D Systems, MAB835, 1:100), Hopx (mouse ...
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bioRxiv - Bioengineering 2024Quote: ... The FL5.12 cell culture media was also supplemented with 100 ng/ml IL-3 (R&D Systems). L1210 cells were obtained from ATCC ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primary antibodies were used: rabbit anti active caspase 3 (1:200; R&D Systems AF835), and rabbit anti-Ki67 (1:5000 ...
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bioRxiv - Immunology 2020Quote: ... and then re-suspended in 30 μL of media containing 10,000 units/mL of recombinant IFN-α2a or 100 units/mL of recombinant IFN-γ (R&D Systems). Antibodies against SiglecF ...
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bioRxiv - Cell Biology 2021Quote: Cytokine array was carried out in the young and aged L-MSC culture media using Proteome Profiler Mouse XL Cytokine Array kit (R&D Systems) as per manufacturer ‘s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... 0.1 % Triton-X-100 in PB (0.1 mol/L, pH 7.4) and incubated with goat anti-CD31 (1:100, R&D systems, Minneapolis, USA). As secondary antibody biotinylated anti-goat IgG (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 2% PBS-BSA and incubated with secondary anti-goat IgG (H+L) APC-conjugated antibody (F0108, R&D systems) in 2% PBS-BSA for 20 minutes at room temperature at 0.05 µg/106 cells for ACE2-A549 cells or at 0.1 µg/106 cells for Vero E6 cells ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
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bioRxiv - Microbiology 2021Quote: ... cells were maintained in SFD media supplemented with the following: 3 μM CHIR 99021 (CHIR, R&D Systems), 10 ng/ml human FGF10 ...
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bioRxiv - Cell Biology 2023Quote: ... after which the cells were exposed for 3 days to 200 ng/ml FGF-9 (R&D systems) and 1μg/mL heparin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with rabbit active Caspase-3 (5 μg/ml, AF835-SP, R&D systems, Inc., Minneapolis, MN), rabbit cleaved Caspase-9 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
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bioRxiv - Neuroscience 2023Quote: ... explants were incubated overnight in PBS 3% BSA with primary antibody: anti-Robo1 (R&D Systems, 1:400) or anti-Robo2 (gift from Dr Alain Chédotal 53 ...
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bioRxiv - Molecular Biology 2020Quote: L-FABP levels in human and mouse sera were measured using enzyme-linked immunosorbent assay kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
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bioRxiv - Cancer Biology 2024Quote: ... Conditioned media was collected from L-Wnt3a cells (ATCC CRL-2647™) and Cultrex HA-R-Spondin1-Fc 293T cells (R&D Systems), and the final organoid media formulation contained 20% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Cell Biology 2023Quote: ... and tissue harvest as previously described and in Supplementary Methods.6 Structural analysis of lung tissue included hematoxylin and eosin stain as well as immunostain for muscularization of vessels as previously described.7 Cytokine levels were measured by pooling plasma and lung lysate from 5 mice treated with either L-NAME/HFD or control water/diet for 5 weeks and was assayed using an antibody-based cytokine array (R&D Systems, ARY006) and was completed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Supernatants (1 mL from individual wells in 24-well plates and 250 μL from individual wells in 96-well plates) were collected and directly used for ELISAs (DuoSet® ELISA kits from R&D Systems). Supernatants from LPS conditions were diluted 1:5 for IL-10 quantification and 1:10 for TNF-□ quantification to avoid reaching the upper limit of detection ...
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bioRxiv - Molecular Biology 2020Quote: ... Mycoplasma testing was performed every 3 months with the MycoProbe mycoplasma detection kit from R&D systems (Minneapolis, MN). None of the cell lines used in this study are found on the misidentified cell lines list from the International Cell Line Authentication Committee.
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bioRxiv - Cancer Biology 2021Quote: ... Negative control cells were produced by treatment with 10 μM Z-DEVD-FMK caspase-3 inhibitor (R&D systems). 200μl of cell suspension from each cell line including the negative control group was transferred into a flow cytometry tube before being treated with 2.5μl of 0.2mM NucViewTM 488 caspase-3 substrate (fluorogenic DNA dye and DEVD moiety substrate for caspase-3 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for Mycoplasma routinely every 3 months using MycoProbe Mycoplasma Detection Kit (R&D Systems).
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bioRxiv - Bioengineering 2019Quote: Stage 1: 3 days in S1 basal media with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF10A cells were treated for 3 days with 5 ng/ml of TGF-β1 (R&D Systems, Minneapolis, MN).
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 μM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... and 50 mmol/L β-mercaptoethanol] supplemented with recombinant mouse stem cell factor (SCF) (12.5 ng/mL; R&D Systems, Cat. No. 455-MC) and recombinant mouse IL-3 (10 ng/mL ...
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bioRxiv - Physiology 2024Quote: ... 50 µL of murine plasma was then used to quantify glucagon concentrations with ELISA by following the manufacturer’s protocol (R&D Systems, cat no. DGCG0). Final glucagon concentrations were calculated based on a standard curve.
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bioRxiv - Genomics 2020Quote: ... YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN 20). To reduce the skewness of phenotype distributions ...
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bioRxiv - Microbiology 2021Quote: ... IFN-<ι>λ1/3 concentrations were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Pathology 2021Quote: ... Gdf8 and Smad2/3 proteins were quantified by ELISA methods (GDF8/Myostatin Quantikine ELISA kit, Cat # DGDF80, R&D Systems, Minneapolis ...
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bioRxiv - Cell Biology 2022Quote: ... NED] and 3U.ml−1 EPO [Roche]) supplemented with 1 ng.ml−1 IL→ 3 and 10 ng.ml−1 SCF (both R&D Systems) and 1 µg.ml−1 doxycycline for 4 days ...