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Citations for R&D Systems :
301 - 350 of 1302 citations for 7 Fluoro 3 oxo 3 4 dihydroquinoxaline 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 million cells (in 100 µL of 1:1 mix of PBS and Cultrex Basement Membrane Extract (Type 3, R&D systems)) were injected into the 4th mammary fat pad of 7-8 weeks old female BALB/c mice ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Primary human lung fibroblasts (passages 3-9) were exposed to 5ng/ml recombinant human TGF-β1 (R&D Systems, 240-B-002), 180μM H2O2 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell lines were validated for pluripotency markers by immunhohistochemical stainings using the Human Pluripotent Stem Cell 3-Color Immunohistochemistry Kit (R&D Systems, SC021) and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... magnetically isolated T cells were resuspended in the complete medium and were seeded in a 10-cm petri dish or T-75 flask at 1–2×106 cells/cm2 and cultured in the presence of 20 ng/mL recombinant mouse interleukin-7 (rmIL-7; 402-ML-020/CF, R&D Systems, Minneapolis, MN) for 24 hr at 37°C in a humidified 5% CO2 atmosphere.
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bioRxiv - Immunology 2021Quote: ... 20ng/ml mouse IL-7 (R&D System 407-ML) and 20ng/ml mouse IL-15 (R&D System 447-ML) ...
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bioRxiv - Immunology 2023Quote: ... IL-7 (10 ng/ml; R&D Systems, 207-IL), and TNFα (4.9 ng/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... CH12F3-2 cells were incubated in culture medium supplemented with10ng/mL IL-4 (R&D Systems #404-ML-050), 1ng/mL TGFβ (R&D Systems #7666-MB-005 ...
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Biophysics 2020Quote: ... cells were changed to long term DMEM/F12 media50 in the presence or absence of 3 ng/ml TGF-β1 (R&D Systems, Abingdon, UK). Media was replenished three times per week ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Bioengineering 2019Quote: hESCs were differentiated to SC-β cells as previously described9 by culturing in the following conditions in order: Stage 1: 3 d with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Neuroscience 2023Quote: ... On days 27 to 40 organoids were fed on alternate days with media containing 20 ng/mL NT-3 (R&D Systems, 267-N3) and 10 ng/mL BDNF (R&D Systems 248-BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Immunology 2020Quote: ... IL-7 and stem cell factor (all from R&D systems) in α-MEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and IL-7 (50 ng/ml, R&D systems, #407-ML). After 2 to 4 weeks ...
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bioRxiv - Biochemistry 2023Quote: ... His10-FLAG-BRD4 (0.2 μL, 7 μM, R&D Systems, SP600100), ubiquitin (10 μL ...
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bioRxiv - Cancer Biology 2024Quote: ... and recombinant human IL-7 (5 ng/mL) (R&D Systems).
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...
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bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human interleukin 4 (IL-4) (R&D Systems), Alpha MEM with nucleosides (StemCell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 2ng/mL rhIL-7 and 0.4ng/mL rhIL-15 (R&D Systems). T cells were transduced 24 hours post-activation with lentivirus encoding an anti-EGFR CAR with 41bb/CD3ζ ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems). For culture ...
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bioRxiv - Immunology 2021Quote: ... Caffeic acid phenethyl ester (CAPE) (R&D Systems), Mitoquinone (MitoQ ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Cell Biology 2023Quote: ... mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S, R&D Systems), APC anti-mouse CD45 Antibody (103111 ...
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bioRxiv - Bioengineering 2023Quote: ... induction of intermediate mesoderm began using 7 µM CHIR99021 (R&D Systems, 4423), a Wnt agonist ...
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bioRxiv - Biophysics 2023Quote: Collagen fibrils were formed by combining rat tail tendon-derived acid-soluble type I collagen (5 mg/mL in 20 mM acetic acid) (CultrexRat Collagen I, R&D Systems) with 400 mM HEPES + 0.02% sodium azide and 1 M NaCl at room temperature to reach final concentrations of 1 mg/mL collagen ...
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bioRxiv - Immunology 2021Quote: ... and interleukin 4 (IL-4; 500 U/mL; R&D Systems). GM-CSF and IL-4 were replenished on day 2 and 4 ...