Labshake search
Citations for R&D Systems :
301 - 350 of 1485 citations for 6 methyl 3 oxo 2 3 dihydropyridazine 4 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Neuroscience 2024Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
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bioRxiv - Neuroscience 2023Quote: ... Interleukin-6 (IL-6) levels were measured by enzyme-linked immunosorbent assay (R&D Systems, D6050). Lactate dehydrogenase (LDH ...
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bioRxiv - Molecular Biology 2022Quote: ... CH12F3-2 cells were incubated in culture medium supplemented with10ng/mL IL-4 (R&D Systems #404-ML-050), 1ng/mL TGFβ (R&D Systems #7666-MB-005 ...
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Biophysics 2020Quote: ... cells were changed to long term DMEM/F12 media50 in the presence or absence of 3 ng/ml TGF-β1 (R&D Systems, Abingdon, UK). Media was replenished three times per week ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Bioengineering 2019Quote: hESCs were differentiated to SC-β cells as previously described9 by culturing in the following conditions in order: Stage 1: 3 d with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Neuroscience 2023Quote: ... On days 27 to 40 organoids were fed on alternate days with media containing 20 ng/mL NT-3 (R&D Systems, 267-N3) and 10 ng/mL BDNF (R&D Systems 248-BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Biochemistry 2020Quote: ... IL-6 (DY7984, R&D Systems), IL8 (ELL-IL8-1 ...
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bioRxiv - Genetics 2023Quote: ... and IL-6 (R&D Systems). Normalization to the dry weight of each colonic explant was done.
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bioRxiv - Physiology 2023Quote: ... IL-6 (R&D Systems DY406); and IL-10 (R&D Systems DY417 and Luminex) ...
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bioRxiv - Microbiology 2024Quote: ... IL-6 (R&D Systems, DY406) and IL-1β (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... interleukin- 6 (IL-6) were determined using enzyme immunoassay (EIA) kits (R&D Systems, Minneapolis, MN, USA). The concentration of the above cytokines in culture medium was determined by comparing their optical density to the standard curve.
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bioRxiv - Bioengineering 2022Quote: ... IL-6 concentration was determined using the Mouse IL-6 DuoSet ELISA Kit (R&D Systems; DY406). Cell transfection was achieved using HiPerfect (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: IL-6 secretion was measured using a Human IL-6 Quantikine ELISA Kit (R&D systems, #D6050) per manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... ELISA kit for IL-6 and recombinant mouse IL-6 wrtr from R&D Systems (Minneapolis, MN). Bicinchoninicacid (BCA ...
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Cancer Biology 2019Quote: ... and IL-6 (M6000B; R&D Systems).
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bioRxiv - Immunology 2021Quote: ... hBTLA-6×His tag (R&D systems) and hLIGHT-6×His tag proteins (made by the methods described above ...
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bioRxiv - Neuroscience 2021Quote: ... or hyper IL-6 (R&D systems) for 24 h immediately after cell plating ...
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bioRxiv - Immunology 2021Quote: ... mIL-6 (50ng/ml; R&D Systems), and hTGF-β (5ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... mouse IL-6 (R&D Systems, M6000B), and HMGB1 (Chondrex ...
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bioRxiv - Immunology 2022Quote: ... recombinant mouse IL-6 (R&D Systems), recombinant mouse IL-21 (R&D Systems) ...
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bioRxiv - Immunology 2023Quote: ... IL-6 and TNFα (R&D Systems), following the instructions of the manufacturer.
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bioRxiv - Immunology 2023Quote: ... IL-6 (7270-IL, R&D Systems), IL-1β (ALX-502-001 ...
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bioRxiv - Cell Biology 2023Quote: ... Hepcidin-25 was purchased from Peptides International and IL-6 from R&D Systems.
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bioRxiv - Immunology 2024Quote: ... IL-6 (DY 406, R&D Systems), IL-β (DY 401 ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...
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bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human interleukin 4 (IL-4) (R&D Systems), Alpha MEM with nucleosides (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Caffeic acid phenethyl ester (CAPE) (R&D Systems), Mitoquinone (MitoQ ...
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bioRxiv - Immunology 2019Quote: ... and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems, Ref. HS600C Lot P178994). All these immunoassays were performed according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... TNFα and IL-6 in supernatants were measured using ELISAs (Mouse TNFα; Mouse CCL5; Mouse IL-6, R&D Systems) according to the manufacturer’s protocols ...
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bioRxiv - Pathology 2021Quote: ... Levels of secreted IL-6 were determined according to the manufacturer’s protocol (R&D Systems, IL-6 Quantikine ELISA Kit).
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bioRxiv - Immunology 2021Quote: IL-6 secretion from RAW264.7 cells was measured using the mouse IL-6 Duoset ELISA kit (R&D systems, UK) according to manufacturer’s procedure.