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Citations for R&D Systems :
301 - 350 of 3683 citations for 6 1 4 dioxa 8 azaspiro 4.5 decan 8 yl 3 hydroxy 2 iminopyrimidin 4 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Concentration of sCTLA-4 was estimated by ELISA (R&D Systems, DY476) or SDS-PAGE/CBB staining with bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-toll-like receptor 4 (TLR4; clone #610029, R&D Systems). All antibodies were titrated using negative control cells that don’t or poorly express the target protein ...
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: ... specimens were stained with anti-4 hydroxynonenal (R&D Systems MSB 3249) or anti 3-nitrotyrosine ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.25 ng/mL recombinant human BMP-4 Protein (R&D Systems).Cells were cultured for 28 hours ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Pathology 2023Quote: ... anti–IL-6 (1:200, AF406NA; R&D Systems), anti-GFAP (1:400 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 1 min at 4°C) pre-incubated with 5 μl TRKB antibody (Goat anti-mouse TRKB Antibody, #AF1494, R&D Systems) and cleared through new Protein-G sepharose tubes ...
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bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde followed by permeabilization and staining with primary antibodies for LOXL2 (1:500, R&D Systems) and Tetramethylrhodamine (TRITC)-conjugated Phalloidin (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against myeloperoxidase (1:150 dilution; R&D Systems, AF3667), citrullinated histone H3 antibody (1:100 dilution ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were then probed with anti-HSD17β1 polyclonal antibody (Abnova, H00003292-M03A, 1:1000 dilution, overnight 4°C; R&D systems, MAB7178 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated overnight at 4°C with one of the following antibodies: FGE (R&D Systems, MAB2779, 1:1000), LAMP1 (DSHB ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... 50μl of mitochondria (1μg/μl protein) were treated with Caspase-8-cleaved tBid from 5-500nM (882-B8-050, R&D Systems, Minneapolis, MN), Bim-BH3 from 10-10,000nM (Ac-DMRPEIWIAQELRRIGDEFNAYYARR-amide ...
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bioRxiv - Microbiology 2021Quote: ... The levels of IL-1β and IL-8 in the conditioned medium were measured by ELISA using the human IL-1β DuoSet (R&D Systems; # DY20105) and the human IL-8 set (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
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bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems, or Shino-Test Corp., Tokyo, Japan) following the manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
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bioRxiv - Cell Biology 2020Quote: ... and BMP4-Lif (10 ng/mL of mouse BMP-4 R&D Systems,Inc. ...
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bioRxiv - Genomics 2020Quote: ... 4 μg normal rabbit IgG (R&D Systems, AB-105-C, lot: ER1212071) and ChIP-grade protein G magnetic beads (Cell Signalling 9006S ...
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bioRxiv - Immunology 2022Quote: ... 30 μg/mL LPS plus 25 ng/mL IL-4 (R&D Systems); or 5 μg/mL LPS ...
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bioRxiv - Cell Biology 2022Quote: ... and human bone morphogenetic protein 4 (hBMP4, 1.5 ng/ml; R&D Systems) together with human vascular endothelial growth factor (hVEGF ...
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bioRxiv - Neuroscience 2022Quote: ... 25 µg/ml human recombinant Bone Morphogenetic Protein 4 (BMP4, R&D Systems) and 55 µM β-mercaptoethanol (Life technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... fibroblasts were treated for 4 days with recombinant human OSM (R&D Systems) at 10 ng/μl or vehicle (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies used to assess pluripotency were anti-OCT3/4 (R&D Systems AF1759), anti-SOX2 (BD 245619) ...
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bioRxiv - Immunology 2022Quote: ... or 4 μg/mL of anti-CCL1 Ab (clone 148113; R&D Systems), was added to the cultures.
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bioRxiv - Immunology 2022Quote: ... 4 μg/mL of anti-CCL5 Ab (polyclonal goat IgG; R&D Systems), or 4 μg/mL of anti-CCL1 Ab (clone 148113 ...
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bioRxiv - Immunology 2023Quote: ... and 10 ng/mL of IL-4 (R&D systems, 402-ML-020)) ...
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bioRxiv - Immunology 2023Quote: ... with 50 ng/ml of IL-4 (R&D systems, Minneapolis, MN, USA) and 50 ng/ml of GM-CSF (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant human TIM-4-HIS (R&D Systems, Cat# 9407-TM-050)) ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C for 16 h and measured via ELISA (R&D Systems). The distribution of TGF-β in Ø2×2 mm and Ø5×2 mm constructs was predicted via FE model (FEBio ...
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bioRxiv - Cell Biology 2024Quote: ... 15 ng/mL BMP-4 (314-BP-050/CF; R&D Systems Inc) and 125 ng/mL Fibroblast growth factor (FGF ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Microbiology 2019Quote: ... BSA (24 - 48 h at 4°C in rotation) for HIS-tagged rPtxS1 and rGαi proteins with mouse monoclonal anti-HIS (1:1000) (R&D Systems, MAB050), for Gαi with mouse monoclonal anti-Gαi (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were kept in serum-free medium for 4 hours prior to stimulation and then incubated with recombinant CSF-1 or IL-34 (R&D Systems) for the indicated time points ...
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bioRxiv - Bioengineering 2023Quote: ... MVN and tumoroid treatments were administered over 4 days through daily media perfusion with an IL8 blocking antibody (20 µg mL-1, #MAB208, R&D Systems), Dexamethasone (5 µM ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Immunology 2023Quote: Supernatants collected from human FM explant experiments were measured for the chemokine IL-8 and cytokine IL-1β by ELISA (R&D Systems, Minneapolis, MN). FM uric acid secretion was measured using a colorimetric assay (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... goatαIL-6 (1:100, AB-206-NA R&D Systems), followed by donkeyαmouse AlexaFluor-488 or goatαrabbit AlexaFluor-546 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...