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Citations for R&D Systems :
301 - 350 of 3496 citations for 4 4 2 Hydroxy ethyl piperazin 1 ylmethyl benzoic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Immunology 2020Quote: ... AA-Mφ were generated by stimulating macrophages overnight with 20 ng/ml IL-4 (R&D Systems, Minneapolis, MN). Reg-Mφ were generated by stimulating macrophages overnight with 100 ng/ml LPS and either 200 nM PGE2 (Cayman Chemical ...
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bioRxiv - Immunology 2021Quote: ... IL-4 in cell culture supernatants or peritoneal washes were determined using DuoSet ELISA Development kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and 4 post PR8 infection with commercially available anti-FcγRIII antibody (100 µg/mouse; clone: 275003, R&D systems) and/or anti-FcγRIV antibody (200 µg/mouse ...
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bioRxiv - Immunology 2023Quote: ... a 50:50 mixture of Matrigel Matrix GF and 4 mg/ml Cultrex Rat Collagen I (R&D Systems). For time-lapse live imaging experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Antigen-retrieved sections were incubated overnight at 4 °C with goat anti-CD45 antibody (R&D Systems, AF114-SP) and rabbit anti-CD68 antibody (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... bone marrow neutrophils (0.3 million) were cultured in the presence of 10 ng/ml IL-4 (R&D Systems) or 10 ng/ml GM-CSF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... and incubated with 5 ng/mL IL-4 (cat. no. 404-ML-010, R&D Systems, Minneapolis, MN, USA) (or vehicle control ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 50 ng/mL human bone morphogenetic protein 4 (hBMP4) (R&D Systems, Minneapolis, Minnesota, USA), 50 ng/mL human vascular endothelial growth factor (hVEGF ...
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bioRxiv - Neuroscience 2024Quote: ... 10 ng/ml Activin A (R&D Systems, 338-AC; stock at 10 µg/ml in 4 mM HCl)) and 24 h after plating as well as every 2-3 days thereafter ...
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bioRxiv - Bioengineering 2024Quote: ... 1 % P/S and 10 ng/ml fibroblast growth factor-2 (FGF-2, R&D Systems). Passage 3 hMSCs were used in this study.
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bioRxiv - Cell Biology 2024Quote: ... 0.2mM ascorbic acid and 1 ng/mL recombinant human FGF (R&D system, 233-GMP-025). The adherent cells were split by trypsinization at 80% confluency and frozen at passage 2 (50% αMEM ...
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bioRxiv - Physiology 2020Quote: ... or Contactin 2 (1:1000, R&D Systems, AF4439). Membranes were incubated with secondary antibody Goat anti-rabbit IgG-HRP (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Dectin-2 (1:100, clone 17611; R&D Systems), TLR2 (1:200 ...
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bioRxiv - Developmental Biology 2024Quote: ... TCF-2/HNF-1 beta ((R&D Systems, AF3330 1:200 for IF), OCT4 (Abcam ...
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bioRxiv - Immunology 2022Quote: ... and MΦs with IL-4 (20ng/ml) and IL-13 (20ng/ml; denoted ‘IL4/13’ for brevity; R&D Systems) in MΦ media ...
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bioRxiv - Biophysics 2021Quote: ... was accessed by testing the hydrolysis of 2.5 µM Substrate V (7-methoxycoumarin-4-yl-acetyl-RPPGFSAFK-2,4-dinitrophenyl, R&D Systems), a previously known fluorogenic substrate of IDE ...
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bioRxiv - Cell Biology 2020Quote: ... ELISA kits (CCL17, DY364, MCP1, DCP00, MDC, DMD00, IL-4, D4050, GM-CSF, DY215) were purchased from R&D Systems. PDGF-AA ELISA kits (EHPDGF ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with FGF2 (4 ug/mL; Biochemistry Department, University of Cambridge) and TGF-β1 (1.74 ug/mL; R&D Systems) using Vitronectin XF (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2023Quote: 10^4 LECs were seeded on 96-well plates pre-coated with 50 μl of Cultrex (3433, R&D Systems) and incubated 24 h in the cell incubator ...
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bioRxiv - Immunology 2024Quote: ... and probed at 4°C overnight with specific antibodies directed against human ACE2 (0.4 μg/mL; R&D Systems, MAB10823) or Mouse ACE2 (0.25 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... monocytes were cultured with 40 ng/ml GM-CSF and 40 ng/ml IL-4 (both from R&D Systems) in complete medium ...
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bioRxiv - Developmental Biology 2024Quote: ... micropat-terned hESC colonies were fixed in 4% paraformaldehyde and stained using anti-human beta-catenin antibody (R&D Systems) and secondary antibodies conjugated with Alexa Fluor 647.
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bioRxiv - Developmental Biology 2024Quote: ... and 4 µm sections were prepared for TdT In Situ Apoptosis Detection (#4812-30-K, R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... 2 µg of recombinant Mac-1 integrin (R&D systems) was incubated with reduced PDI or enzymatically inactive PDI at 10 µM for 30 min at 25°C ...
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bioRxiv - Cancer Biology 2020Quote: ... CTLA-4 and TNF-α levels were quantified by using the commercially available immunoassay kits from R&D systems (Minneapolis, USA) and following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Neuroscience 2021Quote: ... embryoid bodies were plated on Matrigel (0.17mg/mL in DMEM/F12) in NIM with 10uM BMP-4 (R&D Systems #314BP010). At day 16 ...
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bioRxiv - Developmental Biology 2022Quote: ... Definitive endoderm differentiation commenced 3-4 days post-passage onto fresh MEFs using 25 ng/ ml WNT3a (R&D Systems, UK) and 100 ng/ ml Activin-A (Peprotech ...
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bioRxiv - Pathology 2020Quote: ... 4°C) and plasma was analyzed for TNF-α and IL-10 (porcine specific ELISA kit, R&D Systems, Abingdon, UK). For qPCR ...
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bioRxiv - Immunology 2020Quote: ... 511 or 111 coated filters and stimulated for 4 h with 5nM tumor necrosis factor-α (TNF-α; R&D Systems), then human monocytes or monocyte Hoxb8 cells were added ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 1μM DDAO (Thermo) and cultured in full RPMI supplemented with 4 μg/ml CD40L (R&D systems), 100ng/ml BAFF (Peprotech) ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections were labeled overnight at 4°C in a humid chamber with goat anti-mouse CLEC4F (AF2784, R&D systems) and rat anti-mouse/human CD324 (DECMA-1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Immunology 2024Quote: ... U-bottom 96 well plates in Essential 8 medium supplemented with 50 ng/mL BMP-4 (R&D Systems, 314-BP010) and 10 μM Rock inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... medium was changed to experimental medium (Ham’s F-12 supplemented with 4% HS and 1X penicillin/streptomycin [R&D Systems; B21210]) for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... MO) or using 50 μg/ml of LPS plus 50 ng/ml of mouse IL-4 from R&D Systems (Minneapolis, MN). After 4 days in complete RPMI media at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and genomic DNA regions of interest were isolated using 4 μg of antibody against Brachyury (R&D Systems, cat# AF2085, lot# KQP0719121). Complexes were washed ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Immunology 2020Quote: ... in serum-free medium (5 x 106/4 ml of AIM-V) supplemented with M-CSF (50 ng/ml; R&D Systems) over 7 days followed by 18 h treatment with IFN-γ (20 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Microbiology 2020Quote: Secretory leukocyte peptidase inhibitor (SLPI) and Boc-Gln-Ala-Arg-7-Amino-4-methylcoumarin (BOC-QAR-AMC) were obtained from R&D systems. Camostat mesylate was obtained from Sigma Aldrich ...