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Citations for R&D Systems :
301 - 339 of 339 citations for 3 Trifluoromethylthio benzeneboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... one million cells were suspended in 100 µl of 1:1 RPMI and BME-Type 3 (R&D Systems, # 36-320-1002P) per implantation ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Human IL-29/IL-28B (IFN-lambda 1/3) concentration in SARS-CoV-2 infected epithelium basal media was quantified using ELISA technics following manufacturer’s recommendations (R&D systems, Minneapolis, USA). 100 μl of media was used for each point.
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
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bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: goat anti-Human/Mouse Caspase-3 (R&D Systems, Cat# AF-605-SP, 1:100-1:200), guinea pig anti-CNP1 (Synaptic Systems ...
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bioRxiv - Biophysics 2020Quote: ... cells were changed to long term DMEM/F12 media50 in the presence or absence of 3 ng/ml TGF-β1 (R&D Systems, Abingdon, UK). Media was replenished three times per week ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... On days 27 to 40 organoids were fed on alternate days with media containing 20 ng/mL NT-3 (R&D Systems, 267-N3) and 10 ng/mL BDNF (R&D Systems 248-BD) ...
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bioRxiv - Bioengineering 2024Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Microbiology 2020Quote: ... Primary BMDCs were isolated from C57BL/6 mice and cultured in Dulbecco’s modified Eagle medium for 3–5 days in the presence of 20 ng/mL recombinant granulocyte-macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA) as described previously (83) ...
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bioRxiv - Microbiology 2020Quote: ... were cultured in 24-well tissue dishes for 3 days in RPMI1640 medium supplemented with 100ng/ml Activin A (R&D Systems, 338-AC-050) and 2µM CHIR99021 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981; IFNλ2/3: R&D systems, MAB1587), or mock ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Neuroscience 2024Quote: ... HEK-293 cells 72h posttransfection or adult DRG neurons at DIV 3 were incubated with commercial anti-CASPR2 antibodies (AF5145, 1:250, R&D Systems, Minneapolis, MN, US) and/or human patient serum (1:50-1:250 ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... HSC cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA; for 3 d), the ROCK inhibitor Y27632 (10 μM ...
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bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
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bioRxiv - Neuroscience 2024Quote: ... cultures were cotreated with 20 nM (2S,3S)-2-Amino-3-methyl-N-[2-(4-morpholinyl)ethyl]pentanamide dihydrochloride (LM11a-31)(R&D Systems, Minneapolis, MN, USA, Cat no: 5046) or vehicle solution consisting of DMSO during application of 6-OHDA ...