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Citations for R&D Systems :
3401 - 3450 of 3502 citations for Mouse Anti Cytomegalovirus Glycoprotein H gH 0861 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 1:1000 diluted in PBS followed by a secondary fluorochrome anti-rabbit NorthernLights NL637-conjugated antibody (NL005; R&D systems, 1:200). Sections were then mounted with Fluormount G containing DAPI (Southern Biotech) ...
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bioRxiv - Microbiology 2022Quote: The location of DC-SIGN was assessed at the surface of BHK-21 cells (not permeabilized) by flow cytometry using an anti-DC-SIGN phycoerythrin-conjugated antibody (FAB1621P R&D Systems) according to a standard procedure (Lozach et al. ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 µg/ml, R&D Systems, MAB17781) for 1h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... cochlear frozen mid-modiolar cross sections or whole mounts were incubated overnight at room temperature with combinations of following primary antibodies: goat anti-CD45 (R&D Systems, catalog # AF114 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... NK cells were also co-cultured with K562 or P815 coated with anti-NCR (NKp30, NKp44, NKp46, R&D Systems, 5ug/ml) or anti-CD16 (clone 3G8 ...
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bioRxiv - Developmental Biology 2024Quote: ... of medium samples were analysed by SDS-PAGE under reducing conditions and underwent Western blotting using anti-Wnt5a antibody (MAB6452, R&D systems). Immune signals for exogenously added Wnt5a in the medium and cell lysate were quantified using ImageJ ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer and stained in 50 µL for flow cytometry using the following antibodies: anti-CCR2 (475301, R&D Systems), anti-CD11b (M1/70 ...
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bioRxiv - Immunology 2023Quote: ... 600,000 cells plus culture medium were replaced in a 24 well plate containing a 50-60% confluent monolayer of unstimulated ADSCs with or without addition of anti-human IL-1β (2ug/mL, R&D Systems) or a mouse IgG1 isotype control (2ug/mL ...
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bioRxiv - Immunology 2022Quote: ... while A29L antigen was detected with rabbit polyclonal NR-627 primary (BEI Resources) and an anti-rabbit IgG-HRP (R&D Systems). Data analysis was performed using Compass software (ProteinSimple/Bio-Techne).
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with affinity-purified polyclonal goat anti-huACE2 antibody recognizing the extracellular domain (1:40 dilution, #AF933, R&D Systems) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... 25 μl of the supernatant was diluted with 25 μl 1% BSA PBS-T and added to a Nunc Maxisorp plate coated with 1 μg/ml anti-C5a capture antibody (C5a DuoSet ELISA kit, R&D systems). Next ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining volume was divided into two tubes and incubated on ice with either 1 μg of anti-galectin-9 (AF2545, R&D systems) or goat isotype control (Jackson Immunoresearch ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... TMA slides were stained at the histopathology core of Research Institute of McGill University Health Centre (RI-MUHC) using primary antibodies including anti-NE (MAB91671100; R&D Systems), anti-CD66b (392902 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated in blocking solution (3% bovine serum albumin + 0.1% Triton X-100 + 5% Horse serum) at room temperature for 1hr and then with anti-OSMR beta (R&D Systems, AF662) antibody (10 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566; R&D Systems) followed by an Alexa Fluor® 647 conjugated donkey anti-goat monoclonal antibody at a 1:1000 concentration (ab150135 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary antibody at a 1:1000 dilution (HAF107; R&D Systems). As a control ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropili-1 inhibition studies were repeated as described above for EG00229 using a goat polyclonal anti-NRP-1 blocking antibody (AF566; R&D Systems) at 1 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: Exogenous and endogenous GDNF in the medium was neutralized with anti-GDNF antibody (#AF-212-NA, R&D system, Minneapolis, MN, USA). 0.25 ...
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bioRxiv - Bioengineering 2024Quote: ... CAR T cells for the initial challenge day 0 imaging experiment were stained with the following antibody mix in FACS buffer (PBS 1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... cells from re-challenge plates were stained with the following antibody mix in FACS buffer (PBS1x + 2% FCS + 2mM EDTA): anti-RQR8 mAb clone QBEND10-APC (R&D Systems), anti-CD4 mAb clone VIT4-VioBlue (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell extract was then immunoprecipitated overnight at 4°C on a rotating wheel using anti-PKR antibody (71/10, R&D Systems) conjugated to protein A sepharose beads (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with the first primary antibody (an anti-IL-1R1 antibody, 1: 100, MAB269, R&D Systems, Minneapolis, USA). Negative controls were incubated in the same solution without the primary antibody ...
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bioRxiv - Immunology 2021Quote: ... the same protein was labeled using its His-tag by incubating the antigen with an Alexa Fluor 647-conjugated anti-His-antibody (R&D Systems, IC0501R) for 30 minutes at room temperature at a 2:1 ratio (RBD molecules:IgG molecules) ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Detection of bound sIL7R was carried out by one-hour incubation at room temperature with biotinylated goat anti-human IL7R polyclonal antibody (R&D Systems, # BAF306), followed by 30-minute incubation at room temperature with streptavidin-horseradish peroxidase (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 30 min at 20°C in 50 μl RPMI-HEPES in the absence of BCS with 2 μg/ml anti-ACE2 antibody (R&D Systems, #AF933) (Hoffmann et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were blocked in 2% goat serum for 45 mins at room temperature and incubated with anti-Snail (R&D Systems, Minneapolis, MN), anti-Zeb1 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... on ice for 30 minutes and then stained on ice for 1 hour with a 1:100 dilution of goat anti-Epha1 antibody (R&D systems #AF3034). After two washes with PBS/10% DS ...
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bioRxiv - Neuroscience 2019Quote: ... were cut on a cryostat and mounted on slides and processed for GDNF receptor GFRα1 (goat anti-GFRα1, 1:100; cat no. AF-560, R&D Systems, Minneapolis, MN, USA) and P2X5 (rabbit anti-P2X5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then blocked with odyssey PBS for 1 hour at room temperature and incubated with the anti-hERBB2/Her2 AF488 antibody (1:200, R&D systems biotechne) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 2) anti-CD31 antibodies (#550274, BD Biosciences, San Jose, CA, USA; #ab28364, Abcam, Cambridge, MA, USA; #BBA7, R&D systems, Minneapolis, MN, USA); 3 ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocyte-enriched cell suspension was incubated for 20 minutes at room temperature on a dish coated with anti-HepaCAM antibody (R&D Systems MAB4108) to bind astrocytes ...
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bioRxiv - Microbiology 2021Quote: ... One million cells were re-suspend in 100 μl PBS and either immediately incubated with 0.25 μg anti-ACE2 antibody (R&D systems, Cat.# AF933) or first incubated at 37 °C for 6 hours with shaking to allow recovery of cell surface proteins after trypsinization ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated in PBS-T with 1% ELK for 45 minutes with either goat anti-human C1r (1:300, R&D Systems, AF1807), sheep anti-human C1s (1:300 ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Cancer Biology 2021Quote: ... The cells were resuspended in 100 µl total volume and stained with 5 µl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were removed from adherent cultures using AssayComplete cell dissociation reagent (Eurofins #92-0009) and incubated with a polyclonal anti-ALK2 antibody (R&D Systems #AF637) or a polyclonal anti-ALK3 antibody (Sino Biological #50078-RP02) ...