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Citations for R&D Systems :
3351 - 3400 of 3429 citations for Mouse anti Rift Valley Fever Virus Nucleoprotein M976 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropili-1 inhibition studies were repeated as described above for EG00229 using a goat polyclonal anti-NRP-1 blocking antibody (AF566; R&D Systems) at 1 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell extract was then immunoprecipitated overnight at 4°C on a rotating wheel using anti-PKR antibody (71/10, R&D Systems) conjugated to protein A sepharose beads (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with the first primary antibody (an anti-IL-1R1 antibody, 1: 100, MAB269, R&D Systems, Minneapolis, USA). Negative controls were incubated in the same solution without the primary antibody ...
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bioRxiv - Immunology 2021Quote: ... the same protein was labeled using its His-tag by incubating the antigen with an Alexa Fluor 647-conjugated anti-His-antibody (R&D Systems, IC0501R) for 30 minutes at room temperature at a 2:1 ratio (RBD molecules:IgG molecules) ...
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bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Detection of bound sIL7R was carried out by one-hour incubation at room temperature with biotinylated goat anti-human IL7R polyclonal antibody (R&D Systems, # BAF306), followed by 30-minute incubation at room temperature with streptavidin-horseradish peroxidase (Millipore Sigma ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 30 min at 20°C in 50 μl RPMI-HEPES in the absence of BCS with 2 μg/ml anti-ACE2 antibody (R&D Systems, #AF933) (Hoffmann et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were blocked in 2% goat serum for 45 mins at room temperature and incubated with anti-Snail (R&D Systems, Minneapolis, MN), anti-Zeb1 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... on ice for 30 minutes and then stained on ice for 1 hour with a 1:100 dilution of goat anti-Epha1 antibody (R&D systems #AF3034). After two washes with PBS/10% DS ...
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bioRxiv - Neuroscience 2019Quote: ... were cut on a cryostat and mounted on slides and processed for GDNF receptor GFRα1 (goat anti-GFRα1, 1:100; cat no. AF-560, R&D Systems, Minneapolis, MN, USA) and P2X5 (rabbit anti-P2X5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then blocked with odyssey PBS for 1 hour at room temperature and incubated with the anti-hERBB2/Her2 AF488 antibody (1:200, R&D systems biotechne) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 2) anti-CD31 antibodies (#550274, BD Biosciences, San Jose, CA, USA; #ab28364, Abcam, Cambridge, MA, USA; #BBA7, R&D systems, Minneapolis, MN, USA); 3 ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocyte-enriched cell suspension was incubated for 20 minutes at room temperature on a dish coated with anti-HepaCAM antibody (R&D Systems MAB4108) to bind astrocytes ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
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bioRxiv - Microbiology 2021Quote: ... One million cells were re-suspend in 100 μl PBS and either immediately incubated with 0.25 μg anti-ACE2 antibody (R&D systems, Cat.# AF933) or first incubated at 37 °C for 6 hours with shaking to allow recovery of cell surface proteins after trypsinization ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated in PBS-T with 1% ELK for 45 minutes with either goat anti-human C1r (1:300, R&D Systems, AF1807), sheep anti-human C1s (1:300 ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Cancer Biology 2021Quote: ... The cells were resuspended in 100 µl total volume and stained with 5 µl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were removed from adherent cultures using AssayComplete cell dissociation reagent (Eurofins #92-0009) and incubated with a polyclonal anti-ALK2 antibody (R&D Systems #AF637) or a polyclonal anti-ALK3 antibody (Sino Biological #50078-RP02) ...
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bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ICAM-1 and VCAM-1 protein levels were evaluated by SDS PAGE using goat anti-Icam-1 (R&D systems Cat# AF-796) and rabbit anti-Vcam-1 (abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies and dilutions for human muscle cross section imaging: anti-human PDGFR alpha antibody 1:100 (R&D Systems, #AF-307-NA), anti-Laminin 2 alpha 1:250 (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with a goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN). This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... A multiplex primary antibody mix was prepared in milk-free antibody diluent using Rabbit anti-DYKDDDK antibody (R&D Systems MAB8529; 1:1200) and mouse anti-Beta actin (R&D Systems MAB8929 ...
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bioRxiv - Immunology 2024Quote: ... 1x106 cells then stained in 200 µL on ice for 30 minutes with: Alexa Fluor® 405 anti-IFNα/β R1 (1µg, FAB245V, R&D Systems), PE anti-IFNα R2 (1µg ...
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bioRxiv - Neuroscience 2023Quote: ... PGRN measurements were made using the Mesoscale Discovery (MSD) platform as previously described (106) using either a biotinylated anti-hPGRN antibody (R&D Systems, BAF2420) or anti-mPGRN antibody (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... Trem2 measurements were made using the Mesoscale Discovery (MSD) platform as previously described (29, 105) using a biotinylated anti-mTrem2 polyclonal antibody (R&D Systems BAF1729) as capture antibody and a sulfo-tagged detection antibody (sheep anti-mouse Trem2 ...
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bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubated with anti-8-oxo-dG antibody (1:400 in 1% goat serum; R&D Systems, 4354-MC-050, Lot# P275856) for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Cancer Biology 2023Quote: AREG-neutralising experiments were performed by pre-incubating freshly prepared EVs (at 4 x 103 EVs per recipient cell) with either 4 µg/ml of goat anti-AREG (R&D Systems #AF262) or goat anti-IgG (R&D Systems #AB-1080-C ...
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bioRxiv - Cell Biology 2024Quote: ... slides for ACE-2 single stain and slides for ACE-2 stain were incubated with the Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100 ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2021Quote: ... every third sagittal section of tissue containing the left fastigial nucleus was blocked with 5% bovine serum albumin for one hour at room temperature prior to incubation in primary antibody goat anti-osteopontin overnight at 4°C (1:300, R&D Systems AF808; RRID:AB_2194992). Tissue was then rinsed and incubated in donkey anti-goat Alexa Fluor 594 for 2 hours at room temperature (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific blocking for 30 minutes in 8% horse serum in KPBS was completed before incubation in primary antibody (anti-HPLN1 1:150, R&D Systems 2608-HP) for 48 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Ad-transduced cells were incubated for 24h before performing flow cytometry staining for surface expression of hACE2 using goat anti-human mAb AF933 (R&D Systems, Minneapolis, MN) used at a final concentration of 10μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) with 0.1% Tween 20) for 30 minutes and probed with primary antibodies: polyclonal goat anti-C3d (R&D systems, AF2655, 1:1000), polyclonal sheep anti-Sez6L2 (R&D Systems ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed and stained as described in (Rijal et al. 2019) using a 1:500 dilution of anti-CD66 (#MAB2244, R&D Systems, Minneapolis, MN), anti-CD62-L (#555542 ...