Labshake search
Citations for R&D Systems :
3351 - 3400 of 3472 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP) ...
-
bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
-
bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
-
bioRxiv - Genomics 2023Quote: ... was applied for 30 minutes at room temperature followed by primary antibody staining overnight at 4°C using anti-CD206 (R&D systems, AF2534), anti-CD11b (Cell Sciences ...
-
bioRxiv - Immunology 2024Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
-
bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were cultured for 3 d (days 0–3) using SFD medium supplemented with 10 μM Y-27632 and 100 ng/mL recombinant Activin A (R&D Systems, Cat# 338-AC-010). For anterior foregut endoderm induction (days 3–5) ...
-
bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
-
bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
-
bioRxiv - Developmental Biology 2020Quote: ... 50 ng/mL FGF1 and 5 μg/mL heparin or 10 ng/mL PDGF-AA (R&D Systems #1055-AA-050) supplemented DMEM for 12 hrs.
-
bioRxiv - Developmental Biology 2020Quote: ... Micromass cultures were then maintained at 37°C in a humidified incubator with 5% CO2 for 24hrs with the inhibitors before treatment with 5ng TGFβ1/ml (R&D Systems) for the indicated times.
-
bioRxiv - Cell Biology 2021Quote: ... cells were treated with increasing doses of TGFβ2 (vehicle control, 1.25 ng/ml, 2.5 ng/ml, and 5 ng/ml; R&D Systems, Minneapolis, MN) for 48 h ...
-
bioRxiv - Microbiology 2023Quote: ... 100 µg/ml streptomycin and either 5 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Abingdon, UK) to promote M1-differentiation or 20 ng/ml macrophage colony-stimulating factor (M-CSF ...
-
bioRxiv - Bioengineering 2024Quote: ... and stained with a 5 µg/mL of donkey anti-goat IgG NorthernLights™ NL637-conjugated antibody (R&D Systems; NL002) for 30 minutes at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
-
bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
-
bioRxiv - Immunology 2024Quote: ... 5×104 labeled cells were resuspended in B cell media (RPMI, 5%FCS, 50μM 2-mercaptoethanol and 2mM glutamine) and stimulated with 50ng/ml CD40L (R&D Systems), 50ng/ml IL-4 (STEMCELL Technologies) ...
-
bioRxiv - Cell Biology 2024Quote: ... , and 5 uM of PfHEUL (PF3D7_0826100) HECT domain were incubated with 10 mM ATP in ubiquitin conjugation reaction buffer (R&D systems). All reactions were incubated at 37C for 1 hour ...
-
bioRxiv - Physiology 2024Quote: ... Protein expression was visualised using immunofluorescence staining of mesenteric artery rings and the following antibodies: anti-CD31 (PECAM, # AF3628, R&D Systems, 1:1000 dilution, raised in goat); anti-TRPV4R (# ACC-034 ...
-
bioRxiv - Cancer Biology 2023Quote: To measure IFNγ secretion by the T cells co-cultured with the tumor-derived macrophages in the media of the T cell suppression assay we used Quantikine™ ELISA Mouse IFN-γ Immunoassay (R&D Systems, catalog number #MIF00-1) according to manufacturer instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... with mTsER™ Plus media supplemented with the SMAD inhibitors SB-431542 (Medchem, Cat# HY10431; 10 μm final) and Dorsomorphin (R&D Systems. Cat# 309310; 1 μm final), and the Rho-associated proteinase kinase (ROCK ...
-
bioRxiv - Bioengineering 2024Quote: ... and inflammatory factors (TNF-α, IL-1β, IL-6, and MCP-1) in different therapeutic agents were measured by Quantikine® ELISA kit (R&D Systems Inc. Minneapolis, MN, USA) in triplicate ...
-
bioRxiv - Cancer Biology 2024Quote: ... the measurement was paused and then 5 µL of a 1 mg/mL solution in PBS was added (human recombinant CD206, Cat # 2534-MR-050/CF from R&D systems, reconstituted with 50 µL of mQ); final concentration of CD206 in the cell ...
-
bioRxiv - Immunology 2021Quote: ... 115-006-068), 250ng/ml mouse recombinant MEGACD40L (Enzo, ALX-522-120) and 20ng/ml Recombinant mouse IL-4 (R&D system, 404-ML). Cells were collected at specific time points for analysis ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
-
bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
-
bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
-
bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
-
bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
-
bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
-
bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
-
bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
-
bioRxiv - Bioengineering 2022Quote: MONHA-encapsulated hydrogels were cultured in MONHA growth medium for 21 days followed by treatment with increasing doses of TGFβ2 (vehicle control, 2.5 ng/ml, 5 ng/ml, and 10 ng/ml; R&D Systems, Minneapolis, MN) for 7 days ...
-
bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
-
bioRxiv - Immunology 2021Quote: Supernatant samples were collected and pre-diluted 5-fold before quantification of cytokines by ELISA assays targeting IL-1β (R&D systems) or IL-18 (Abcam ...
-
bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for the indicated amount of time ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5 µm sections were deparaffinized and stained with either hematoxylin and eosin or with specific antibodies for PROX1 (R&D Systems, AF2727) and GFP (Abcam ...
-
bioRxiv - Microbiology 2020Quote: ... fragments were washed three times in 5 ml cold AdDF+++ before being embedded in 30 μL BME (Type 2; R&D Systems) per well in a 48-well plate ...
-
bioRxiv - Cell Biology 2021Quote: ... Culture was continued for 24 h in low serum medium with 0 or 5 ng/ml TGF-β1 (R&D Systems, www.rndsystems.com) with emodin (0 ...
-
bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
-
bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
-
bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
-
bioRxiv - Cell Biology 2022Quote: ... freshly isolated RBCs in basal medium supplemented with 10 mM HEPES at 20% hematocrit were incubated for 30 minutes under 37 °C with gentle shaking with 5 μg/mL ADAMTS13 (R&D Systems) [27 ...
-
bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
-
bioRxiv - Cancer Biology 2021Quote: SUM159-LM1 cells or MDA231-LM2 cells were suspended in HuMEC/0.1% BSA and treated with EC-derived conditioned media (CM) (diluted 2-5 times in the final medium) or 50 ng/ml of recombinant activin B (R&D systems). 2,500 cells/well of SUM159-LM1 cells or 5,000 cells/well of MDA231-LM2 cells were seeded on 96-well ultra-low attachment plate (Corning) ...
-
bioRxiv - Biochemistry 2020Quote: ... a total of 1 μg native proMMP2 and 0.5 μg proMMP14 were separately incubated in 10 μl with a range of furin (catalog no. 1503-SE-010, R&D systems) concentrations (0 – 250 nM ...
-
bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...