Labshake search
Citations for R&D Systems :
3301 - 3350 of 3441 citations for Mouse Anti Chlamydia LPS 1681 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... with or without 1.25 µg/mL Scl-Ab or 500 ng/mL anti-M-CSF antibody (#AF416 from R&D Systems). After 8 days ...
-
bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
-
bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
-
bioRxiv - Immunology 2019Quote: ... Tissue was washed and when needed incubated with secondary fluorescently labeled antibodies (Donkey Anti-Rabbit Pacific Blue or Alexa Fluor 647) for 2h before nuclear staining with Draq-7 (R&D Systems) or 4′,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Neuroscience 2021Quote: ... The ELISA was performed according to the published protocol [43] using these antibodies: anti-TrkB goat 1:1000 (#AF1494, R&D systems), anti-SorCS2 rabbit 1:2000 (#bs-11963R BIOSS) ...
-
bioRxiv - Molecular Biology 2021Quote: Retinal organoids on day 90 of differentiation were incubated at RT for 1 hour in PBS with either a goat anti-ACE2 antibody (AF933, R&D Systems) or a normal goat IgG control (AB-108-C ...
-
bioRxiv - Immunology 2019Quote: ... anaesthetized C57BL/6 mice were first injected with 50 µl anti-LYVE1-Alexa Fluor 488 antibody (20 µg/ml, R&D Systems) into the rear right hock ...
-
bioRxiv - Immunology 2019Quote: ... 96-well flat bottom plates were pre-coated with 20 μg/ml of an agonist anti-TREM-1 mAb (clone 174031; R&D Systems) overnight at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... of LPS treated rats were double-immunostained for the analysis of the cellular localization of cathepsin X using the following primary antibodies: goat polyclonal anti-cathepsin X antibody (1:75, R&D System), mouse monoclonal anti-TH antibody (1:500 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The overexpression and loss of ICAM-1 protein were validated by flow cytometry with an anti-ICAM1 antibody (BBA20, R&D Systems). Besides ...
-
bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
-
bioRxiv - Biochemistry 2021Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
-
bioRxiv - Microbiology 2020Quote: ... cells were wash three times with PBS to remove any residual FBS and followed by 1-hour treatment with goat anti-human ACE2 antibody (R&D Systems) or anti-hDDP4 antibody (R&D Systems ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were re-suspended in 100 μl of FACS buffer and incubated with 10 μl of PE-conjugated anti-sheep IgG secondary antibody (F0126, R&D systems) for 30 min on ice ...
-
bioRxiv - Immunology 2020Quote: ... The nitrocellulose membrane was blocked in 5 % non-fat milk in PBS-T for 2 hours at room temperature and probed overnight with goat anti-hACE2 primary Ab at 1 μg/mL (AF933, R&D systems). Following three wash intervals of 10 minutes with PBS-T ...
-
bioRxiv - Microbiology 2021Quote: ... The Hoc proteins each eluted as a single monomeric peak and were run on reducing SDS-PAGE and verified by anti-His western (R&D Systems) (Supplementary Fig.3) ...
-
bioRxiv - Developmental Biology 2022Quote: ... microglial cells were preincubated with 20µg/ml of anti-C3aR antibody (Catalog # MAB10417) or control IgG (R&D Systems, Inc. Minneapolis, MN) suspended in DMEM/F12 supplemented with 5% heat-inactivated FBS ...
-
bioRxiv - Molecular Biology 2020Quote: Randomly selected sections coming from ovarian tissue of least 3 different individuals were used for immunohistochemistry for Anti-active Caspase-3 (AC-3, 1:1000, AF-835, R&D Systems), γH2AX (1:250 IHC-00059 ...
-
bioRxiv - Microbiology 2021Quote: ... the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG HRP-conjugated Ab (HAF016) from R&D system; mouse anti-HA mAb (16B12) ...
-
bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
-
bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
-
bioRxiv - Cancer Biology 2019Quote: ... and the corresponding cryostat tissue sections (8-10μm thickness) were stained using the following primary antibodies: goat anti-CD31 (R&D System; AF3628), rabbit anti-caspase-3 (Sigma ...
-
bioRxiv - Developmental Biology 2019Quote: ... A biotin conjugated anti-His-tag antibody (NB100-63172, Bio-Connect) was added followed by a Streptavidin-HRP (DY998, R&D Systems). For a colormetric detection ...
-
bioRxiv - Cell Biology 2019Quote: ... Incubation with primary rat-polyclonal antibody against LYVE-1 in combination with rat-polyclonal biotinylated anti-MHC-II antibody (both R&D Systems) was done for 2h at room temperature ...
-
bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed with PBST three times and incubated with 30 μl of biotinylated anti-rabbit secondary antibody for 90 minutes (1:10 dilution, 865002, R&D Systems). After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution ...
-
bioRxiv - Developmental Biology 2019Quote: ... Commercially available anti-FLT1 antibodies were obtained from the manufacturer in carrier free and preservative free form (AF471 from R&D Systems, D2 from Santa Cruz Biotechnology ...
-
bioRxiv - Biochemistry 2021Quote: ... Selective isolation of MLC1-complex from the PM was achieved by cs-IP using anti-GlialCAM Ab (1:2000, R&D Systems). Ab was bound on ice to live cells for 45min ...
-
bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
-
bioRxiv - Microbiology 2021Quote: ... The surface expression of hACE2 was confirmed by flow cytometry using anti-human ACE2 AlexaFluor 647-conjugated Ab (R&D Systems, # FAB9332R). The expression was further confirmed by immunoblot using hACE2 Ab (Cell Signaling Technology ...
-
bioRxiv - Immunology 2021Quote: ... were coated with anti-IFN-γ antibody (capture mAb; 1 µg/mL) according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). Plates were blocked using culture medium (DMEM containing 10% FBS ...
-
bioRxiv - Immunology 2021Quote: ... followed by 1 hour incubation in 3% nonfat milk in Tris-buffered saline with 0.1% Tween 20 detergent (TBST) buffer with horseradish peroxidase-conjugated anti-rabbit (1:2000, R&D Systems, HAF008) or mouse (1:2000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.1 % Triton-X-100 in PB (0.1 mol/L, pH 7.4) and incubated with goat anti-CD31 (1:100, R&D systems, Minneapolis, USA). As secondary antibody biotinylated anti-goat IgG (1:500 ...
-
bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
-
bioRxiv - Cell Biology 2022Quote: ... followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems, catalog #: PS022), a mouse anti-GABAA receptor β2/β3 subunit antibody (Millipore ...
-
bioRxiv - Immunology 2022Quote: ... 1:1000 diluted in PBS followed by a secondary fluorochrome anti-rabbit NorthernLights NL637-conjugated antibody (NL005; R&D systems, 1:200). Sections were then mounted with Fluormount G containing DAPI (Southern Biotech) ...
-
bioRxiv - Microbiology 2022Quote: The location of DC-SIGN was assessed at the surface of BHK-21 cells (not permeabilized) by flow cytometry using an anti-DC-SIGN phycoerythrin-conjugated antibody (FAB1621P R&D Systems) according to a standard procedure (Lozach et al. ...
-
bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
-
bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 µg/ml, R&D Systems, MAB17781) for 1h at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... cochlear frozen mid-modiolar cross sections or whole mounts were incubated overnight at room temperature with combinations of following primary antibodies: goat anti-CD45 (R&D Systems, catalog # AF114 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
-
bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
-
bioRxiv - Immunology 2024Quote: ... NK cells were also co-cultured with K562 or P815 coated with anti-NCR (NKp30, NKp44, NKp46, R&D Systems, 5ug/ml) or anti-CD16 (clone 3G8 ...
-
bioRxiv - Developmental Biology 2024Quote: ... of medium samples were analysed by SDS-PAGE under reducing conditions and underwent Western blotting using anti-Wnt5a antibody (MAB6452, R&D systems). Immune signals for exogenously added Wnt5a in the medium and cell lysate were quantified using ImageJ ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer and stained in 50 µL for flow cytometry using the following antibodies: anti-CCR2 (475301, R&D Systems), anti-CD11b (M1/70 ...
-
bioRxiv - Immunology 2023Quote: ... 600,000 cells plus culture medium were replaced in a 24 well plate containing a 50-60% confluent monolayer of unstimulated ADSCs with or without addition of anti-human IL-1β (2ug/mL, R&D Systems) or a mouse IgG1 isotype control (2ug/mL ...
-
bioRxiv - Immunology 2022Quote: ... while A29L antigen was detected with rabbit polyclonal NR-627 primary (BEI Resources) and an anti-rabbit IgG-HRP (R&D Systems). Data analysis was performed using Compass software (ProteinSimple/Bio-Techne).
-
bioRxiv - Microbiology 2023Quote: ... Cells were incubated with affinity-purified polyclonal goat anti-huACE2 antibody recognizing the extracellular domain (1:40 dilution, #AF933, R&D Systems) for 30 minutes ...