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Citations for R&D Systems :
3201 - 3250 of 3477 citations for Mouse Vasoactive intestinal polypeptide receptor 2 VIPR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Eluate fractions were analyzed by SDS-PAGE and immunoblot using an anti-histidine tag antibody (Monoclonal Mouse IgG1 histidine tag antibody; R&D Systems, cat. no. MAB050). Fractions containing TDO2 were combined and dialyzed (molecular weight cutoff 3.5 kDa ...
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bioRxiv - Neuroscience 2024Quote: ... 10-week-old control female and 13-weekold control male mice (Esr1fl/fl) were injected daily with recombinant mouse NOV/CCN3 at 7.48 μg/kg (R&D Systems Catalog # 1976-NV-050) or vehicle control (0.9% NS) ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 2.5% BSA/0.1% Triton-X for 1 hour and subsequently incubated with an antibody cocktail consisting of goat anti-mouse PDGFRα (1:100) (catalog #: AF1062; R&D Systems; Minneapolis, MN, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Microbiology 2024Quote: ... Cytokine levels within the lung and brain homogenates of infected mice were analyzed using the Proteome Profiler Mouse XL Cytokine Array (R&D Systems Inc., Minneapolis, MN). The relative expression levels of 111 soluble mouse proteins were determined following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Developmental Biology 2022Quote: ... re-plated and incubated in 10-cm non-adherent Petri dishes in cSFDM containing 2 ng/ml rhActivin A (R&D Systems, #338-AC), 3 ng/ml rhBMP4 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Bioengineering 2020Quote: Proteome Profiler Antibody Array Kits for human angiogenesis factors (R&D systems, MN, USA) were used for reverse western blot ...
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bioRxiv - Cancer Biology 2020Quote: ... and human XL oncology array kit (R&D Systems, Proteome profiler, Cat. No: ARY026), which detects 84 human cancer-related proteins (Table S3) ...
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bioRxiv - Cancer Biology 2021Quote: ... Total protein was extracted using the Human Phospho RTK-array kit (R&D Systems) lysis buffer ...
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bioRxiv - Immunology 2022Quote: ... and IFNγ and TNFα levels were measured with appropriate immunoassay Kits (R&D Systems) according to the manufacturer’s instructions.
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Potential of host serum protein biosignatures in the diagnosis of tuberculous meningitis in childrenbioRxiv - Immunology 2019Quote: ... ferritin and MMP-9 were assessed in Luminex kits purchased from R&D Systems Inc ...
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bioRxiv - Cancer Biology 2020Quote: The Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, Minneapolis, MN, USA) was purchased and phosphorylation profiles of kinases were analyzed according to the manufacture’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... and Anti-rat HRP-DAB Cell & Tissue Staining Kit (CTS017) from R&D Systems were used.
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bioRxiv - Cancer Biology 2021Quote: The Proteome Profiler Human Phospho-Kinase Array Kit was purchased from R&D Systems. THP1 cells were lysed ...
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bioRxiv - Immunology 2021Quote: ELISpot kits to measure the secretion of human IFN-γ (R&D Systems, #EL285) were used according to manufacturer’s protocols ...
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bioRxiv - Systems Biology 2022Quote: ... avidin and biotin blocking reagents (HRP-DAB Cell &Tissue Staining Kit, R&D Systems) and washing in TBST in between each blocking step ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A customed kit for pro-fibrotic biomarker detection was ordered from R&D systems. The experiment was performed according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... A bead-based multiplex cytokine kit (LXSARM-17, R&D Systems, Minneapolis, MN, USA) was used to measure CSF levels of CXCL2 ...
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bioRxiv - Immunology 2024Quote: The spinal cord sections were retrieved by Universal Antigen Retrieval Kit (R&D Systems) in a steamer for 15 min at 100°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were verified mycoplasma negative using the MycoProbe Mycoplasma Detection Kit (R&D Systems). For serum-starvation experiments ...
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bioRxiv - Immunology 2024Quote: ... and IL-1β was assessed using a kit from R&D systems (Minneapolis, MN).
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bioRxiv - Immunology 2024Quote: ... Supernatants were thawed and subjected to a custom multiplex bead kit (R&D Systems) to assess the levels of released analytes including IL-21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Immunology 2024Quote: ... #CCL-131) were plated in each well of 96-well plates and preactivated with 100 ng/mL mouse IFN-γ (R&D Systems, #485-MI-100) for 18-24 hours ...
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary incubation was 1 hour at RT in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, HAF007 and HAF008, respectively). The suspension for blocking and antibody incubations was 1x Tris buffered saline (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... After 48 hours the cells were fed with stage 2 differentiation medium consisting of STEMDiff APEL supplemented with 50 ng/mL of VEGF 165 (R&D Systems, 293-VE-010), 25 ng/mL of BMP4 (R&D Systems ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) and incubated at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 107 cells per sample were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and incubated with 50 µg/mL SARS-CoV-2 spike S1 subunit-Fc fusion protein (R&D systems, #10623-CV-100) or human IgG1 isotype control (BioXCell ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
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bioRxiv - Microbiology 2021Quote: ... Memory CD4+ T-cells were infected with HIV-1 (50 ng HIV-p24/106 cells) and then cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA) for up to 9 days ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were resuspended in 1 part Advanced DMEM/F12 and 2 parts Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems; 3533-010-02) for a final solution of 66% Cultrex ...
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bioRxiv - Microbiology 2023Quote: ... organoids were mechanically disrupted into single cells and plated in droplets of Cultrex growth factor reduced BME type 2 (Biotechne | R&D systems 3533-010-02). For the 2D culture of airway organoids ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... washed gently three times with warm DMEM-F12 and refed with mTeSR without ROCKi for 2 hours followed with mTeSR containing 50ng/ml BMP4 (R&D systems, #314-BP-010) for 42-48 hours ...
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bioRxiv - Bioengineering 2024Quote: ... were transferred into Neural Induction Media (NIM) composed of DMEM/F-12 supplemented with 1x N-2 MAX Media Supplement (R&D Systems, Cat no. AR009), 1x GlutaMAX ...