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Citations for R&D Systems :
3151 - 3200 of 4535 citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-Hevin (1:2000; R&D Systems AF2836), mouse anti-SPARC (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... anti-CD31/Pecam1 (R&D Systems 1:200, Dako), and F4/80 (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... anti-Goat Cd13 (R&D systems, AF2335, 1:500); anti-Rat Ly6b2 (BioRad ...
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bioRxiv - Physiology 2024Quote: ... CXCR2-APC (5μg mL-1; 242216; R&D Systems), CD44-BV570 (0.3μg mL-1 ...
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bioRxiv - Immunology 2022Quote: ... NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL rhIL-2 (R&D Systems, Cat. 202-IL-010). NK cells were then placed into a 37°C incubator ...
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bioRxiv - Pathology 2020Quote: ... cells of 2 MOI were treated with serum-free media for 24 hours and then media containing TGF-β (20 ng/ml, R&D Systems, Minneapolis, MN, USA) for 72 hours ...
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bioRxiv - Physiology 2024Quote: Wild type C57BL/6 10-week-old female mice were treated daily with recombinant tumor necrosis factor-like weak inducer of apoptosis (TWEAK 1237-TW; R&D Systems; 2 ug per mouse) for 4 days followed by a single i.p ...
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bioRxiv - Immunology 2024Quote: ... 2fTGH and HEK293T cells and ELISA was performed according to the manufacturer’s protocols for human IFN-β (R&D systems DY814-05PBL Assay Science 41410-2), human IFN-γ (Biolegend 43104 ...
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bioRxiv - Cancer Biology 2021Quote: ... was then diluted in water to 50 uL in a 96-well microtiter plate and 10 uL Malachite Green Reagent A was added per manufacturer instructions (R&D Systems Cat# DY966). After 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Neuroscience 2020Quote: ... L-glutamine and 10 ng/ml of carrier-free murine recombinant granulocyte and macrophage colony-stimulating factor (GM-CSF, 415-ML, R&D systems, Minneapolis, MN). Cell suspension from one brain was plated in a T75-cell culture flask coated with poly-D-lysine (P7280 ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of IP10 in the media was then determined by ELISA using the Mouse CXCL10/IP-10/CRG2 DuoSet ELISA from R&D system (cat. DY466) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-10 were assayed using a Luminex mouse pre-mixed Multi-Analyte kit according to standard instructions (LXSARM-06, R&D System, Minnesota, USA).
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bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in appropriate cell culture medium as directed by supplier with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems; Minneapolis, MN) and 1% Penicillin-Streptomycin (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... Seven different ligand conditions were tested at concentrations previously determined to elicit maximal cell responses49 (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM) ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes from SP and draining LNs were cultured in the presence of IRBP-p (10 μg/ml) and IL-23 (R&D systems, Minneapolis, MN) (10 ng/ml ...
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bioRxiv - Physiology 2023Quote: ... Then the tumor necrosis factor alpha (TNFA) and interleukin 10 (IL10) protein levels in the tissue were measured by enzyme linked immunosorbent assay (ELISA) (R&D Systems, Abingdon, UK).
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bioRxiv - Immunology 2023Quote: Concentrations of CXCL10 in K18-hACE2 mice were measured in serum and lung supernatant collected at euthanasia using the Mouse Magnetic Luminex Assay kit for mouse CXCL10/IP-10/CRG2 (R&D Systems LXSAMSM-01). Both serum and supernatant samples were diluted 1:2 in the kit’s Calibrator Diluent RD6-52 then utilized in the assay procedure provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... Myeloperoxidase (MPO, indicative of tissue oxidation) levels in ALF (using 10 μg of protein) were determined by using LUMINEX (R&D Systems, Minneapolis, MN).
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bioRxiv - Immunology 2022Quote: ... and tubulin were detected by immunoblotting using HIV-1 gag p24 antibody (1:1000 dilution, MAB7360, mouse monoclonal, R&D systems, Minneapolis, MN), human CD207 antibody (1:1000 dilution ...
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bioRxiv - Genomics 2021Quote: ... or PermWash (SOX1) prior to incubation with primary antibody (anti-EpCAM at 1/10k, abcam ab71916; anti-SOX1 at 1/300, R&D Systems AF3369) for 45 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1, 1:50,000, R&D Systems – AF3787). After 2x10 minutes washes with milliQ water and 2x10 minutes washes with TBS-T ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies consisted in the human anti-spike mAb 48 (1:1,000; a gift from H. Mouquet) or the mouse anti-p24 Gag MAB7360 (R&D Systems; 1:1000). Anti-human or mouse IgG secondary antibodies ...
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bioRxiv - Pathology 2020Quote: ... IGF-1 was measured in the samples using a R&D Systems Mouse 1-Plex Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... on ice for 30 minutes and then stained on ice for 1 hour with a 1:100 dilution of goat anti-Epha1 antibody (R&D systems #AF3034). After two washes with PBS/10% DS ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then blocked with odyssey PBS for 1 hour at room temperature and incubated with the anti-hERBB2/Her2 AF488 antibody (1:200, R&D systems biotechne) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated in PBS-T with 1% ELK for 45 minutes with either goat anti-human C1r (1:300, R&D Systems, AF1807), sheep anti-human C1s (1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... Some sections were stained with antibodies to early endoderm (GATA6-CST 5851 1:1600) neurectoderm (Beta3 tubulin R&D Systems MAB1195 1:200) or smooth muscle actin in mesoderm (alpha SMA R&D Systems MAB1420 1:200 ...
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bioRxiv - Physiology 2022Quote: ... or immunofluorescent (IF) staining as indicated below: Antibody against VE-cadherin extracellular domain (WB 1:1000, IF 1:50, R&D Systems, MAB9381), antibody against VE-cadherin intracellular domain (WB 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:500, rabbit anti-PDGFRα (3164 Cell Signalling Technologies, Danvers, Massachusetts) 1:500 and goat anti-Sox10 (AF2864 R&D Systems, Minneapolis, Minnesota) 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Sections were then blocked with 10% normal goat serum and incubated with primary antibodies against Iba1 (1:500, Wako Chemicals, USA) and IL-1β (1:1000, R&D Systems, USA) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubated with anti-8-oxo-dG antibody (1:400 in 1% goat serum; R&D Systems, 4354-MC-050, Lot# P275856) for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was probed with an anti-EFA6R rabbit polyclonal antibody (1:500) (8) or an anti-β-actin mouse monoclonal antibody (1:10,000) (R&D Systems, UK) diluted in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... followed by incubation with primary antibodies (rabbit-anti RFP, Rockland, diluted 1:1500 and goat anti-PDGFRα, R&D Systems, diluted 1:500) in 1% BSA ...
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bioRxiv - Immunology 2023Quote: ... The remaining NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL (250 IU/mL) rhIL-2 (R&D Systems, cat. no. 202-IL-010), then placed in a 37C CO2 incubator for 12-16 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were starved with 2% FBS media overnight and treated with Activin-A (R&D system, 338-AC-010, 25 ng/mL or 50 ng/mL) for indicated times.
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bioRxiv - Cancer Biology 2020Quote: ... were added to devices with THP-1 M1 macrophages while matrix metalloproteinase 7 (MMP7, clone 111433 at 1 μg/ml) and ANGPT2 (clone 85816 at 1 μg/ml) (all from R&D systems, Minneapolis, MN) neutralizing antibodies were added to devices with THP-1 M2 macrophages ...
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bioRxiv - Immunology 2024Quote: ... anti-AMPKα2 (#AF2850, diluted at 1:1000) and anti-IL6 (#AB-206-NA, diluted at 1:500) antibodies from R&D Systems (Minneapolis, MN, USA). Secondary antibodies used for immunofluorescence labelling of fixed cells were goat anti-rabbit antibodies conjugated to Alexa Fluor 488 (#11034 ...
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bioRxiv - Physiology 2022Quote: ... Plasma ICAM-1 and VCAM-1 were measured using the Mouse ICAM-1/CD54 and VCAM-1/CD106 Quantikine ELISA kits according to the handbooks (R&D Systems, Minneapolis, USA).
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... female BALB/c mice were anaesthetized and injected as described above with 1 μg CINC-1 protein (453-KC-050/CF, R&D systems, Abingdon, UK) in 1 μL saline in the left striatum ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4℃ with the following primary antibodies: anti-Nestin (rabbit, 1:500, Millpore, PN-MAB5326) and anti-ki67 (mouse, 1:500, R&D system, PN-AF7649); anti-CHI3L1 (rabbit ...
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bioRxiv - Physiology 2019Quote: ... Cells were washed with BEC medium containing 10% FBS and were re-suspended in Flow Cytometry Staining Buffer (1X) (R&D Systems, Minneapolis, MN, USA) supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse SCLC cells were seeded into 6-well plates and cultured for 16 h in RPMI1640 + 10% BGS with or without 100 ng/mL of Wnt5a (R&D System, 645-WN-010) or recombinant Wnt3a (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% FBS in the presence of FMS-like tyrosine kinase 3 ligand (Flt3L) (100 ng/ml, R&D Systems, Cat# 308-FK) for 7-9 days ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... the media was changed with full media containing FGF (10 ng/ml bFGF, Sigma Aldrich #F0291; 25 ng/ml rhFGF, R&D Systems #345-FG), BMP-4 (40 ng/ml ...