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Citations for R&D Systems :
3151 - 3200 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and stained with a 5 µg/mL of donkey anti-goat IgG NorthernLights™ NL637-conjugated antibody (R&D Systems; NL002) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Cell Biology 2024Quote: ... , and 5 uM of PfHEUL (PF3D7_0826100) HECT domain were incubated with 10 mM ATP in ubiquitin conjugation reaction buffer (R&D systems). All reactions were incubated at 37C for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... MO) or using 50 μg/ml of LPS plus 50 ng/ml of mouse IL-4 from R&D Systems (Minneapolis, MN). After 4 days in complete RPMI media at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... and genomic DNA regions of interest were isolated using 4 μg of antibody against Brachyury (R&D Systems, cat# AF2085, lot# KQP0719121). Complexes were washed ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... supplemented with recombinant human IL-4 (50 ng/mL) and recombinant human GM-CSF (100 ng/mL) (R&D Systems, Minneapolis, MN) with one additional supplement of fresh media and cytokines at day 3 of culture as previously described 12 ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x 106 BM cells were incubated at 37°C or 4°C for 45 min with or without Cxcl12 (10 nM, R&D Systems). The reaction was stopped by adding ice-cold RPMI and brief centrifugation at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Adherent cell were then cultured in RPMI supplemented with 10% heat-inactivated human serum and L-Glutamine and gentamycin as above (RPMI10%HuS) and 30 ng/ml GM-CSF (Immunex) and 10 ng/ml IL-4 (R&D Systems) for 5 days50 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Neuroscience 2023Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
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bioRxiv - Physiology 2024Quote: ... the platelets were incubated at 4°C over night with antibodies against CXCR4 (MAB172, Clone # 44716, R&D Systems, Minneapolis, Minnesota, USA), ACKR3 (ab72100 ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked for 1 h with 5% dried skim milk in TBS containing 0.1% Tween 20 (T-TBS) and incubated overnight at 4°C with specific primary mAbs and pAbs: Nluc (mouse mAb; R&D Systems) for Nluc-expressing viruses ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell extract was then immunoprecipitated overnight at 4°C on a rotating wheel using anti-PKR antibody (71/10, R&D Systems) conjugated to protein A sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 4 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Physiology 2024Quote: ... in 0.1% Tween-20 in PBS and incubated in the following antibodies overnight at 4°C: Goat anti-GLI3 (1μg/ml, R&D Systems, #AF3690) or Rabbit anti-alpha-tubulin (1μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... 5 ml of serum (collected and combined from n=4 mice) was used to probe the Mouse Cytokine Proteome Profiler Array (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... All of the experiments with MEFs were performed at passage 4 (P4) and at 60-70% confluence on glass fibronectin (1030-FN-05M, R&D Systems) coated coverslips or plastic culture plates (Starlab).
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bioRxiv - Cell Biology 2024Quote: ... TGF-β1 was reconstituted in sterile 4 mM HCl + 0.1% BSA according to manufacturer’s instructions (R&D Systems Cat #240-B-002). Indicated conditions were treated with the TGF-β1 inhibitor SB 431542 (Sigma-Aldrich Cat# S4317 ...
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bioRxiv - Biochemistry 2021Quote: ... cells were resuspended in Goat anti-Human ACE2 antibody (1:500, R&D Systems) in the flow buffer ...
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bioRxiv - Cell Biology 2020Quote: ... anti-goat IgG HRP-conjugated (1: 1000, HAF 017, R&D Systems, Minneapolis, USA), anti-rabbit IgG HRP-conjugated (1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies used were rat anti-CDH1 (R&D Systems FAB7481F, paraffin, 1:300), rabbit anti-P63 (Proteintech 12143-1-AP ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with anti-tubulin βIII antibody (R&D systems, Tuj1 clone, 1:1000). Extent of axonal repulsion was measured by the ratio between the lengths of the proximal axons (i.e. ...
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bioRxiv - Neuroscience 2022Quote: ... transferred onto PVDF membranes and probed with anti-Pax3 (1:250; R&D systems) and then anti-β-actin (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... or 1 μg/mL of goat anti-mouse PDGFRα antibody (R&D Systems, AF1062) during the last 48 hours of incubation (on day 7) ...
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bioRxiv - Genomics 2021Quote: ... and incubated with antibodies against Kit (1:200 anti Kit AF1356 R&D Systems). The antigen-bound primary antibodies were detected using 1:500 dilution of biotinylated anti-goat IgG antibody (BA-5000-1.5 ...
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bioRxiv - Genomics 2021Quote: ... Kit protein was detected with 1:2000 anti c-kit (AF1356 R&D Systems) and 1:2000 of anti-goat secondary antibody (sc2020 ...
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bioRxiv - Neuroscience 2020Quote: ... the following antibodies were used: anti-human Nestin (R&D Systems, MAB1259, 1:2,400), goat anti-TrkB (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: ... anti-phospho-STAT1 (p-STAT-1, Tyr701; AF2894; R&D Systems, Minneapolis, MN, USA) and anti-STAT1 (66545-1-Ig ...
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bioRxiv - Bioengineering 2020Quote: ... and 2.5 ng mL−1 recombinant mouse FGF2 (R&D Systems # 3139-FB-025). In some experiments ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies and dilutions used: goat anti-Lumican (R&D Systems, AF2745, 1:200); rabbit anti-Lumican (Abcam ...
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bioRxiv - Pathology 2021Quote: ... 1% antibiotics and 10 ng/ml basic fibroblast growth factor (bFGF) (R&D Systems). Human MPCs were trypsinized and incubated 30 min with biotinylated anti-human PDGFRα (R&D system ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with goat pAB anti-hACE2 (1:50, AF933 R&D Systems) and mAB anti-SARS-CoV-2-spike (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Purified ICAM-1 Fc Chimera was from R&d systems (Stock concentration 50ug/ml). Poly clonal Anti-ICAM-1 produced in Rabbit was from Santa Cruz (Stock concentration of 200ug/ml) ...
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bioRxiv - Immunology 2021Quote: ... and goat anti-Granzyme B ab (R&D systems, AF1865, at 1:50 dilution) in above blocking solution overnight at +40C ...
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bioRxiv - Neuroscience 2022Quote: ... the following antibodies were used: Goat anti-human NANOG (R&D systems, 1:200), rabbit Oct-4A (Cell Signaling Technology ...
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bioRxiv - Pathology 2022Quote: ... and PE-conjugated anti-Tissue Factor/CD142 (R&D Systems, polyclonal, #FAB3178P, 1/50).
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bioRxiv - Cell Biology 2022Quote: ... and anti-rabbit HRP-conjugated secondary antibody (R&D Systems, HAF008; dilution 1:2000). Bio-rad Precision Plus Protein Dual Color Standards (in kDa from top to bottom of the gel ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 hour with blocking buffer (Reagent Diluent, R&D Systems, UK). Plates were washed 3 times with wash buffer and 100 ul of samples and standards were added (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies used were anti-TUJ1 (1:600, mouse monoclonal, R&D Systems, #MAB1195); anti-Substance P (SP ...
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bioRxiv - Cell Biology 2020Quote: ... and probed with primary antibodies: goat anti-PDGFRα (1:1000; AF1062, R&D Systems), rabbit anti-TCF4/TCF7L2 (C48H11 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the WNT signalling pathway inhibitor IWR1-endo (1 ng/ml, R&D Systems), which was refreshed after 48 h ...