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Citations for R&D Systems :
3151 - 3200 of 4278 citations for 7 7R 7 Amino 5 azaspiro 2.4 hept 5 yl 8 chloro 6 fluoro 1 1S 2R 2 fluorocyclopropyl 1 4 dihydro 4 oxo 3 quinolinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with 1 ng/mL of recombinant human TGF-β1 (R&D Systems), 10 ng/mL of recombinant mouse IL-4 (R&D Systems ...
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bioRxiv - Physiology 2020Quote: Fecal lipocalin-2 levels in distal colon fecal contents were analyzed using Mouse Lipocalin-2 DuoSet ELISA (R&D Systems, DY1857) according to manufacturer provided instructions ...
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bioRxiv - Immunology 2020Quote: ... Lcn-2 levels were quantified in the supernatants using the Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Molecular Biology 2021Quote: Recombinant human transforming growth factor-β (TGF-β) and fibroblast growth factor-2 FGF-2 were purchased from R&D Systems. BTZ ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl biotinylated rhIL-2 or biotinylated control protein (from Fluorokine® Biotinylated Human IL-2 Kit, R&D Systems) added to each well ...
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bioRxiv - Bioengineering 2023Quote: ... Human IL-2 in the supernatant was measured using a sandwich ELISA kit (Human IL-2 Quantikine ELISA, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The protein level of LCN-2 was analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems). The protein level of TNFα was measured using the Mouse TNFα ELISA kit following the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: ... and then were seeded into upper chamber of transwell inserts (8 μM pore size, Costar) with or without coated Matrigel (R&D System). The lower chamber of the transwell was filled with 750 μl medium supplemented with 10% FBS which function as a chemoattractant ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Microbiology 2021Quote: Protein expression was analyzed by polyacrylamide gel electrophoresis on 8%-16% precast gradient gels (Thermo) and Western blotting using antibodies to ACE2 (AF933, R&D Systems), c-Myc-epitope (clone 9E10 ...
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bioRxiv - Cancer Biology 2019Quote: ... and the corresponding cryostat tissue sections (8-10μm thickness) were stained using the following primary antibodies: goat anti-CD31 (R&D System; AF3628), rabbit anti-caspase-3 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... It was counted as Day 0 when medium was changed to SF medium with 8 ng/mL recombinant Activin A (R&D Systems), 5 ng/mL recombinant human VEGF 165 Protein (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed EVs were concentrated with 3 kDa MWCO centrifugal filters and characterized via Luminex Multiplex Assay for TNF-α and IL-8 according to the manufacturer’s instructions (R&D Systems). Results were normalized by total protein content ...
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bioRxiv - Cell Biology 2024Quote: ... and MCP-1 were determined in efflux collected on day 8 using a custom version of the Human XL Cytokine Performance Panel (R&D systems). SHBG levels were quantified using the Human XL Cytokine Discovery Panel (R&D systems) ...
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bioRxiv - Cancer Biology 2022Quote: ... cocultures were treated with 3 μg/ml IL1α-neutralizing antibody (#MAB4001, R&D Systems) or isotype control antibody (#400902 ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human WNT3A and R-spondin 3 were from R&D Systems (Minneapolis, USA).
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... FMS-like tyrosine kinase-3 ligand (FLT3-L) (100 ng/mL, R&D systems) and thrombopoietin (TPO ...
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bioRxiv - Immunology 2024Quote: ... 2mM L-Glutamine and 20ng/mL of recombinant human IL-3 (R&D systems). Cas9 and gRNA components were assembled into RNP per the manufacturer’s instructions and transfected into pDC 16 hrs p.c ...
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bioRxiv - Microbiology 2023Quote: Calu-3 cells were treated with exogenous β-IFN (R&D Systems, Minneapolis MN), baricitinib (Selleck Chemicals ...
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bioRxiv - Cell Biology 2020Quote: ... and IL-6 (assay detection range 9.38-600 pg/ml) were purchased from R&D Systems (Minneapolis, MN, USA) and were used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The specificity of staining was confirmed by staining with rat IgG (ref:6-001-F, R&D Systems, Biotechne) or rabbit IgG (ab27478 ...
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bioRxiv - Developmental Biology 2021Quote: ... into chondroprogenitor cells (6 days; 20 ng/ml BMP4 [cat. num. 314-BP-010CF; R&D Systems, Minneapolis, MN]). Mesodermal differentiation medium had a base of Iscove’s Modified Dulbecco’s Medium ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatants were collected and assayed for IL-6 and TNF-α by ELISA Kits (R&D Systems, Minneapolis, MN).
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bioRxiv - Bioengineering 2020Quote: ... supernatants were collected and IL-6 and TNF-α levels were determined using DuoSet ELISA kits (R&D Systems) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Mouse IL-6 was measured in cell supernatants using a DuoSet ELISA kit per manufacturer instructions (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... Mouse IL-6 was detected from cell culture supernatants by ELISA according to the manufacturer’s instructions (R&D Systems).
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in cell supernatants was determined by ELISA following the manufacturer’s instructions (R&D Systems). Results were read in a Synergy Mx plate reader (BioTek).
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bioRxiv - Immunology 2022Quote: ... PPS14-TT together with recombinant IL-6 (500 ng/adult and 100 ng/neonate (R&D systems, Minneapolis, MN) was emulsified with aluminum hydroxide ...
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bioRxiv - Physiology 2022Quote: ... mouse IgG2a kappa (eBM2a) (eBioscience™, 14-4724-82) and the rat IgG (R&D Systems, 6- 001-F).
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bioRxiv - Physiology 2024Quote: ... Then 6 hours later NRVMs were treated with PBS or 100ng/mL IGF1 (291-G1-01M; R&D Systems) to elicit physiological hypertrophy for 48 hours prior to cell harvesting for experiments ...
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bioRxiv - Immunology 2020Quote: ... We used commercially available ELISA kits to quantify plasma IFNγ (mouse IFNγ platinum ELISA extra sensitive, ebioscience), CXCL10 (mouse IP-10 platinum ELISA, ebioscience) and Angiopoietin-2 (mouse/rat Angiopoietin-2 quantine ELISA kit, R&D Systems). Absorbance was measured using a Multiskan Ascent (MTX Lab systems ...
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bioRxiv - Bioengineering 2019Quote: ... 2013] consisting of the osteogenic media supplemented with 100 ng/mL BMP-2 (recombinant human BMP-2, 355-BM-050, R&D Systems). Media was replaced every 3 days ...
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bioRxiv - Biochemistry 2021Quote: Human anti-SP IgG or ACE-2 Fc was mixed with recombinant SARS-CoV-2 spike RBD His tag (R&D Systems) or recombinant SARS-CoV-2 spike RBD (N501Y)-His tag (Sino Biological ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of LCN-2 in the supernatant was measured by using a DuoSet Mouse Lipocalin-2/NGAL ELISA (R&D Systems) kit ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were analyzed for lipocalin-2 content using Quantikine ELISA kit for mouse Lipocalin-2/NGAL (R&D Systems, Cat: MLCN20).
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
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bioRxiv - Cell Biology 2020Quote: ... The beads were removed by centrifugation and the phosphorylated GST-NEPHRIN and GST-NEPH1 were mixed with recombinant purified active SHP-2 (Recombinant Human SHP-2; Cat. No.: 1894-SH; R&D Systems) in the presence of phosphatase buffer (10mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... one hundred microliter of pre-warmed complete medium containing 10 ng/mL recombinant mouse IL-2 (rmIL-2; 402-ML-020/CF, R&D Systems) or 20 ng/mL rmIL-7 was added to each Nucleocuvette™ strip well for activated or naïve CD8+ T cells ...
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bioRxiv - Immunology 2021Quote: ... were measured by flow cytometry 2 days after activation with or without an extra stimulation with 25 ng/mL IL-2 (R&D Systems) during 15 min.
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bioRxiv - Immunology 2021Quote: ... fifty microliters of stimulant pepmixes (JPT peptides) resuspended in RPMI+10%FBS (R10) with recombinant mouse IL-2 (rmIL-2) (R&D Systems) were added to each well ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and used to quantify fecal lipocalin-2 levels using the mouse lipocalin-2/NGAL DuoSet ELISA kit (R&D Systems) in a microplate reader according to manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... TGFB2 dosage in each eye (13 controls + 2 cases) was conducted with the Human TGF-beta 2 Quantikine ELISA kit (R&D Systems), following the provided instructions.
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bioRxiv - Cancer Biology 2021Quote: ... and heparin sulfate (2 μg/mL; R&D Systems). BTSC lines were confirmed to match their parental primary GBM tumor tissue by short tandem repeat profiling (Calgary Laboratory Services and Department of Pathology and Laboratory Medicine ...
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bioRxiv - Immunology 2021Quote: ... or rSARS-CoV-2 spike protein (R&D Systems). To detect total IgG or IgA expressing B cells ...