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Citations for R&D Systems :
3151 - 3200 of 3301 citations for 6 Bromo 3 4 dihydro 2H 1 benzopyran 3 methanamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... membranes were incubated with horseradish peroxidase⍰conjugated secondary antibody (hCD55 and hCD39, rabbit IgG, 1:5000 (Cat. no. HaF008, R&D Systems); hTBM and β-actin ...
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... or N protein of SARS-CoV-2 (dilution 1:2000) or SARS-CoV-2 membrane ab (R&D Systems, Cat no. MAB10690). Followed 3 time washing with PBST (PBS with 0.1 tween 20 ...
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bioRxiv - Molecular Biology 2024Quote: ... or various doses (100 ng/mL, 200 ng/mL, 400 ng/mL) of recombinant mouse IGF-1 (catalog #: 791-MG-050; R&D Systems) for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems).
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bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910), or MCP-1 (MAB679, clone 23007) obtained from R&D Systems. For patient sample analysis using the immunostorm chip 10 µL of diluted human plasma (1:10 dilution with 1x PBS ...
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bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with the first primary antibody (an anti-IL-1R1 antibody, 1: 100, MAB269, R&D Systems, Minneapolis, USA). Negative controls were incubated in the same solution without the primary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with ITGB4 antibody conjugated to Alexa Fluor® 488 (5 μl/1 million cells) (R&D Systems, Minneapolis, MN, USA) and Propidium Iodide Ready Flow™ Reagent (1 drop/1 million cells ...
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bioRxiv - Immunology 2019Quote: ... PBMCs at a cell density of 1×106 cells/ml were stimulated with a mixture of 25 ng/ml human IL-10 (R&D Systems, UK), 0.5 µg/ml of pokeweed mitogen (PWM) ...
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bioRxiv - Bioengineering 2021Quote: ... 36 million cells were used for sorting with the Sertoli cell marker Thy-1 Cell Surface Antigen (THY1/CD90) using a PE-conjugated antibody for THY1/CD90 (R&D Systems, FAB2067P) and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
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bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 5 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of IL-1β conjugate (R&D Systems, UK). Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of detection antibody (R&D Systems, UK). Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP ...
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bioRxiv - Immunology 2020Quote: ... were seeded at a concentration of 1 × l06 cells/ml in RPMI 1640 medium supplemented with 20 ng/ml GM-CSF (415-ML-050, R&D Systems, USA). After 7 d of culture ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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bioRxiv - Cell Biology 2019Quote: ... 2 ug/ml Doxycycline and 3.34 ug/ml Puromycin, 1 × N27 supplement, and 10 ng/ml BDNF (STEMCELL, 78005) and GDNF (R&D Systems, 212-GD)) ...
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bioRxiv - Cancer Biology 2019Quote: ... CCN4 concentration in conditioned medium was determined by ELISA using Human WISP-1/CCN4 DuoSet ELISA Development Kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Physiology 2020Quote: ... 0.1 mL/10 g body weight) on day 1 and leptin (2 mg/kg; 0.1 mL/10 g body weight; CAT# 498-OB, R&D Systems, Minneapolis, MN) on day 2 at 6am ...
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bioRxiv - Neuroscience 2019Quote: ... were cut on a cryostat and mounted on slides and processed for GDNF receptor GFRα1 (goat anti-GFRα1, 1:100; cat no. AF-560, R&D Systems, Minneapolis, MN, USA) and P2X5 (rabbit anti-P2X5 ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 1 µM SB431542 (S4317, MilliporeSigma, Burlington, MA, USA) to inhibit TGFβR1 activity or 3µM SIS3 (5291, Tocris/R&D Systems, Minneapolis, MN, USA) to inhibit SMAD3 solubilized in 50 mM DMSO ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
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bioRxiv - Immunology 2021Quote: ... Primary antibody (Anti-mouse RAE-1 pan-specific antibody, recognizing RAE-1α, β, δ, γ and ε (R&D Systems, Catalogue Number AF1136) or an appropriate isotype-matched control (polyclonal goat isotype IgG ...
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bioRxiv - Pathology 2022Quote: ... was seeded with 10 000 myoblasts in serum-free medium while the wells were filled with either proliferating medium or serum-free medium complemented with 10 ng.mL-1 FGF-b (R&D Systems 3139-FB), IGF-I (R&D Systems 791-MG ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... A multiplex primary antibody mix was prepared in milk-free antibody diluent using Rabbit anti-DYKDDDK antibody (R&D Systems MAB8529; 1:1200) and mouse anti-Beta actin (R&D Systems MAB8929 ...
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Genomics 2024Quote: ... Progenitors were then resuspended in a 10% dilution in volume of FBS and 100ng/mL CSF-1 (R&D Systems; 216-MC-500) in Gibco™ RPMI-1640 media (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... alone or sequentially with 100 μL/mL polylysine and 400 ng/mL netrin-1 (R&D systems, catalog number 6419-N1-025/CF), and cultured in Neurobasal Medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived-dendritic cells (moDC) were obtained by incubation of monocytes with 1 ng/mL of granulocytes-macrophages colony-stimulating factor (GM-CSF, R&D Systems, France) and Interleukin-4 (R&D Systems ...
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bioRxiv - Pathology 2022Quote: ... for 1 hour at room temperature followed by overnight incubation with primary antibodies for IL-31RA (15 µg/ml, R&D Systems AF2769) and CHRM3(1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurospheres were transferred to Matrigel-coated plates and cultured in Neural Medium (DMEM/F12 medium supplemented with N2, N2, and 20 ng ml-1 of basic FGF (R&D Systems Inc.)) for two weeks to generate a monolayer of hNPCs ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies targeting the following proteins were used at the indicated dilutions and obtained from the denoted companies: Robo3 1:300 (R&D systems, #AF3155), GluN1 NMDAR 1:1000 (SySy #114011) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Developmental Biology 2023Quote: pH 6.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: pH 9.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), SOX9 (Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... Total VEGFR2 expression (0.1% Tx-100 permeabilized cells) was compared to surface level VEGFR2 (non-permeabilized cells) using a biotinylated VEGFR2 antibody (R&D Systems; BT10543). Images were collected using a W1 spinning disk confocal microscope.
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies and dilutions for human muscle cross section imaging: anti-human PDGFR alpha antibody 1:100 (R&D Systems, #AF-307-NA), anti-Laminin 2 alpha 1:250 (Abcam ...
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with an equal volume of 1 mg/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2h ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981 ...
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bioRxiv - Cell Biology 2024Quote: ... slides for ACE-2 single stain and slides for ACE-2 stain were incubated with the Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100 ...
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bioRxiv - Bioengineering 2024Quote: ... one T25 tissue culture flask was given media spiked with 1 ng/mL recombinant human TNF-α (R&D Systems, Cat. # 210-TA) (stimulated group ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with assay medium supplemented with 10 ng/ml transforming growth factor-beta 1 (hereafter referred to as TGF-β, 240-B-002, R&D Systems) for 24 h or 72 h (Peinado et al. ...