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Citations for R&D Systems :
3001 - 3050 of 3520 citations for Human Serine threonine Protein Kinase 17B STK17B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were immobilized on CM5-Sepharose chips coated by standard amine coupling chemistry to a density of about 600 response units (RU) Measurements were carried out at 25°C by injecting 250 μl of recombinant human TNFa (R&D Systems) at concentrations from 0.2 to 50 nM with a flow rate of 35μl/min ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Microbiology 2021Quote: ... the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG HRP-conjugated Ab (HAF016) from R&D system; mouse anti-HA mAb (16B12) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... coated with a recombinant form of the human P-selectin ectodomain (spanning the 42-771 sequence of the preprotein) commercially available from R&D Systems by adaptation of a published protocol (Bally et al. ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems). After 3 days of ActA treatment EBs were plated on fibronectin (20 μg/ml ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Cell Biology 2021Quote: Isolated human platelets were incubated at room temperature for 15-20 minutes with either recombinant HMGB1 (0-40 μg/ml; R&D Systems), ADP (0-5 μM ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... The following antibodies were purchased and used for immunostaining of human sample using staining protocols similar to those used in mouse sections: CD31 (AF806, R&D systems), CXCR4 (2B11 ...
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bioRxiv - Immunology 2020Quote: Titration of blocking antibodies was performed by using static adhesion assays in which activated CD4+ T cells were allowed to adhere to either recombinant human ICAM/Fc or VCAM/Fc chimeras in the presence of blocking antibodies specific for integrin α4 (clone 2B4, R&D Systems) or integrin αL (clone TS1/22 ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...
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bioRxiv - Biochemistry 2019Quote: The corneal micropocket assay was performed as described16 using pellets containing 80 ng of basic fibroblast growth factor (bFGF) or 180 ng of human carrier-free recombinant vascular endothelial growth factor (VEGF; R&D Systems) in C57BL/6J mice ...
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bioRxiv - Genomics 2021Quote: ... Isolated B cells were cultured in IMDM medium supplemented with 10% FBS and 55 mM beta-mercaptoethanol at 1X106 cell/mL and activated by CellXVivo Human B cell expander (1:250 dilution, R&D system) and 50 ng/mL IL2 (Cat#200-02-10ug ...
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bioRxiv - Cell Biology 2020Quote: ... The beads were removed by centrifugation and the phosphorylated GST-NEPHRIN and GST-NEPH1 were mixed with recombinant purified active SHP-2 (Recombinant Human SHP-2; Cat. No.: 1894-SH; R&D Systems) in the presence of phosphatase buffer (10mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175) antibody (catalog no. MAB835; R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IL-10 clone 948505 (MAB9184) and the isotype controls mouse IgG2a (MAB003) and mouse IgG1 (MAB002) (all from R&D systems).
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bioRxiv - Immunology 2020Quote: ... were blocked using 5μg of mouse serum IgG for 10 min at RT then incubated with mouse anti-human ACE2 (R&D systems, FAB9333G), mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... The surface expression of hACE2 was confirmed by flow cytometry using anti-human ACE2 AlexaFluor 647-conjugated Ab (R&D Systems, # FAB9332R). The expression was further confirmed by immunoblot using hACE2 Ab (Cell Signaling Technology ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Cell Biology 2022Quote: ... Both mouse and mixed organoids were exposed to recombinant human IL-11 (rhIL-11, R&D systems, Minneapolis, MN, USA, #218-IL) during the entire organoid culture period of 14 days.
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bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
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bioRxiv - Immunology 2022Quote: ... 1×106 PMBCs were incubated with 20 ng of MIP-1β (recombinant human CCL4L1/MIP-1β isoform LAG-1; R&D Systems) for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Cell Biology 2022Quote: The cDNA encoding the extracellular N-terminal IgV domain of human CD47 (residues 19-141) was amplified from the plasmid hCD47 VersaClone cDNA (RDC1523, R&D systems) and was cloned in the expression vector pGEX-4T-1 (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
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bioRxiv - Immunology 2023Quote: ... 600,000 cells plus culture medium were replaced in a 24 well plate containing a 50-60% confluent monolayer of unstimulated ADSCs with or without addition of anti-human IL-1β (2ug/mL, R&D Systems) or a mouse IgG1 isotype control (2ug/mL ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human FGFs (FGF6 #238F6-025; FGF10-#345-FG-025; FGF12-2246-FG-025) were purchased from R&D systems (Minneapolis, MN).
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... cells from each well were harvested and resuspended in 100 µL of cell resuspension solution provided with human methylcellulose enriched media (R&D systems). Each cell suspension was added to 2.5 mL methylcellulose enriched media ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: Freshly sorted m-LSCs from primary AMLs or prepared CD34+ HSPCs isolated from normal mobilized peripheral samples were plated in human methylcellulose (R&D systems) at about 100K/ml and 2K/ml ...
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bioRxiv - Immunology 2022Quote: Llamas were immunized intramuscularly once per week over six weeks with carrier free recombinant human IL-9 or mouse IL-9 (R&D Systems). Each llama (4 in total ...
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bioRxiv - Developmental Biology 2023Quote: ... EBs were transferred into 60 mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Cancer Biology 2022Quote: Cytokine levels produced by homotypic and heterotypic cultures were analyzed using the Proteome Profiler™ Human Cytokine Array (ARY005B; R&D SYSTEMS) kit ...
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bioRxiv - Immunology 2023Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 5 minutes on ice with a mixture of soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc (R&D systems) for the first scan or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc for the second scan ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...