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Citations for R&D Systems :
2951 - 3000 of 3410 citations for Mouse NLR Family CARD Domain Containing 4 NLRC4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred to a membrane using the iBlot2 system and incubated overnight at 4°C with primary antibody (anti-Gli3, 1:500, AF3690, R&D Systems, Minneapolis, MN). Membranes were then incubated in secondary antibody (anti-goat Alexafluor 680 ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Nunc® Maxisorp™ 384-well plates were coated with 6 µg/ml of recombinant mouse pIgR protein (R&D Systems) diluted in coating solution concentrate (Seracare ...
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bioRxiv - Microbiology 2022Quote: ... KC protein from mouse brain homogenates was detected by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (R&D systems).
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bioRxiv - Cell Biology 2022Quote: ... The collagen-cell mixture was then casted in custom-made chamber and collagen was allowed to polymerize for 45min at 37°C before overlaying the gel with either 0.6ug/ml of recombinant mouse CCL19 (for DCs, 440-M3-025, R&D Systems), 25nM fMLP (for neutrophil-like cells ...
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bioRxiv - Cell Biology 2022Quote: ... Then cells were trypsinized and incubated with APC-conjugated anti-CD140b antibody (2.5 μg/ml, PR7212 clone, Mouse IgG1, R&D Systems) and FITC-conjugated anti-CD146 antibodies (5 μg/ml ...
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bioRxiv - Genomics 2019Quote: 8-week iEPs were stained with mouse E-cadherin-APC antibody (10 μL per one million cells, R&D Systems, FAB7481A) and sorted on a modified Beckman Coulter MoFlo into EcadHigh and EcadLow populations ...
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bioRxiv - Neuroscience 2019Quote: Antibodies and dilutions used for immunofluorescence staining were as follows: polyclonal goat anti-mouse Pdgfra (R&D Systems, AF1062, 1:500), monoclonal rabbit anti-mouse Olig2 (Abcam EPR2673 ...
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bioRxiv - Microbiology 2020Quote: ... rabbit anti-Tuj1 polyclonal antibody (Poly18020, Covance) (1:200),and mouse anti-Tuj1 monoclonal antibody (clone TuJ-1, R&D systems) (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... MSD standard-bind microtitre plates were first coated with 30µl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 µg/ml in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... which was constructed using the cDNA sequences of mouse IL-6/IL-6R alpha fusion protein (9038-SR, R&D systems). AAV8-Null vector was used as vector control.
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bioRxiv - Cell Biology 2020Quote: ... For ICAM-1 cross-linking experiments on ECs we used mouse anti ICAM-1 (Clone # BBIG-I1) from R&D systems and secondary antibody from donkey (DK) ...
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bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag mouse monoclonal antibody (MAB050) and the basic-based antigen unmasking solution (CTS013) from R&D Systems (Minneapolis, MN); anti-FLAG M2 mouse monoclonal antibody (F1804) ...
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bioRxiv - Neuroscience 2021Quote: For sTREM2 measurements, multi array 96-well plates (MSD, L15XA-3) were coated with mouse anti-TREM2 (R&D systems, AF1729) capture antibody O/N at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Neuroscience 2022Quote: ... Group 1 ligands were derived from mouse transcriptomic data and included: APP (R&D Systems, Cat. 3466-PI-010, 20ng/mL), APOE3 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Anti-human CD28 antibody and recombinant mouse ICAM-1/CD54 His-tag were purchased from R&D Systems (Minneapolis, MN, USA). Hybridoma cell lines for mouse anti-CD3 (145-2C11 ...
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bioRxiv - Immunology 2019Quote: ... Some of ITGB4−/− mice were intraperitoneally (i.p.) treated with 150 μg anti-mouse CCL17 monoclonal antibody (mAb, clone 110904, R&D Systems) or isotype control mAb (MAB006 ...
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bioRxiv - Microbiology 2019Quote: ... Mouse and human CXCL1 and CXCL2 as well as human IL-8 (CXCL8) were obtained from R&D Systems (Minneapolis, MN) and multiplex CXCL1 ...
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bioRxiv - Microbiology 2019Quote: ... and KC and IL-1β from mouse brain homogenates were detected by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (R&D systems).
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bioRxiv - Pathology 2020Quote: ... HRP-conjugated mouse anti-IgG (HAF007) and HRP-conjugated goat anti-IgG (HAF017) were purchased from R&D Systems (Minneapolis, USA). Urine protein test kit (C035-2-1) ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with 25 μL of 0.5 μg/mL primary anti mouse-TLR4 (recombinant monoclonal rabbit IgG, clone #1203B, R&D Systems) or isotype (normal polyclonal rabbit IgG control ...
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bioRxiv - Physiology 2020Quote: ... IGF-1 was measured using anti-IGF monoclonal antibody Clone #126002 and anti-mouse IGF-1 Biotinylated Antibody (R&D systems).
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bioRxiv - Molecular Biology 2020Quote: Cytokine levels in cell culture supernatants samples were determined using the Proteome Profiler Mouse Cytokine Array Panel A (R&D Systems) following the manufacturer recommendations.
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bioRxiv - Immunology 2022Quote: The production of 111 cytokines by splenic monocytes was quantified using the Proteome Profiler Mouse XL Cytokine Array (R&D Systems). Cytokine antibody array was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed 3x in 0.1% Triton X-100 in PBS and incubated with the secondary antibodies: anti-mouse IgG2a 647 (Alexa Fluor R&D systems), anti-mouse IgG2a 488 (Alexa Fluor R&D systems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Bioengineering 2024Quote: Purified mouse monoclonal IgG anti-human interferon-γ capture antibody (Cat. #MAB2852, lot #FIO1022021, R&D Systems, Minneapolis, Minnesota, United States), biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech) ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 μl of cell culture media was treated and incubated with the Proteome profiler mouse cytokine array membrane (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Media collected from 3 biological replicates were pooled and a total of 500µL was added to a Mouse XL Proteome Profiler Cytokine Array (#ARY028, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Surface expression of ITFG1 on cells was examined by staining the cells with mouse anti-ITFG1 antibody (R&D Systems, MAB89001) at 4°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: BMDMs were stimulated in complete αMEM with the following proteins as indicated: 20ng/mL mouse IFN-ψ (485MI100, R&D Systems), 20ng/mL mouse IFN-β (8234MB010 ...
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bioRxiv - Physiology 2023Quote: ... Activity was calculated based on a standard curve determined using purified recombinant mouse myeloperoxidase protein (R&D Systems; Catalog# 3667-MP).
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bioRxiv - Cell Biology 2023Quote: Isolated CD8+ DCs were cultured in R10 media supplemented with recombinant mouse FLT3L (250ng/ml; R&D Systems, 427-FL-025). CD8+ DCs isolated from Rubicon-proficient mice were incubated in the presence or absence of 5μM Fendiline-HCl (Tocris ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...