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Citations for R&D Systems :
251 - 300 of 3648 citations for 7 Bromo 5 methyl 1 2 4 benzotriazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced after 48 hours (Day 2) with RPMI-1640 supplemented with B27 without insulin and 5 µM IWP2 (R&D Systems #3533). Media was again replaced after an additional 48 hours (Day 4 ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated overnight at 4°C in the following primary antibodies: T-LCK (1:1000 R&D Systems), T-LCK (1:1000 Proteintech) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were stained overnight at 4°C with mouse CD45 antibody (1:200; Af114, R&D Systems). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were labeled with anti-human SSEA-4 APC-conjugated antibody (1:100, R&D Systems) at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 25 ng/ml bone morphogenetic protein-4 (BMP-4, R&D Systems) and 1 μM all-trans Retinoid Acid (RA ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 ng/mL bone morphogenetic protein 4 (BMP-4) (R&D System) for five days ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-4 (IL-4) (50 ng/mL) (R&D systems).
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... caspase-3 (R&D Systems, AF835), Endomucin (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA) or human IgG isotype control (#31154 ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 ng/mL recombinant human IL-7 (R&D Systems; Bio-techne, Inc.) until use.
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bioRxiv - Cell Biology 2023Quote: ... positive selection marker: Mouse anti-integrin alpha 7 Alexa fluor 647 (FAB3518R, R&D Systems). Cells were rinsed ...
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bioRxiv - Neuroscience 2023Quote: HiPSCs-derived microglia at day 7 of differentiation were stimulated with IFNγ (R&D Systems) 100 ng/ml for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and differentiating spermatogonia marker c-KIT (day 7; 0.6 µg/ml, R&D Systems AF2944) overnight at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: Stage 1: 3 days in S1 basal media with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 ng/ml recombinant human NRG1-β 1 (#396-HB, R&D Systems, USA) as described in Poitelon et al ...
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bioRxiv - Bioengineering 2021Quote: ... Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000, R&D Systems, Cat#MAB10474) was used for the detection of SARS-CoV-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 1:10 ratio and plated on 2-well comet slides (R&D Systems). Cells were then lysed overnight and immersed in alkaline unwinding solution as per manufacturer’s protocol (Trevigen) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... and 1000 IU/ml of interleukin 4 (IL-4; R&D Systems, Minneapolis, MN) to differentiate them into immature dendritic cells (iDC ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were incubated overnight at 4°C with anti-Gremlin1 antibody (R&D systems, AF956, 1:1000) or anti-βActin (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were incubated overnight at 4 °C with goat anti Dscam (AF3666, 1:500, R&D systems) and mouse anti-tubulin (12G10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight at 4°C with primary antibodies against pLCK (Y394) (1:1000) (R&D Systems), GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated overnight at 4°C with antibodies against ACE2 (1:400; R&D systems, Minneapolis, MN, USA), TMPRSS2 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell pellet was stained for 1 hour at 4°C with primary ACE2 antibody (R&D systems #AF933 ...
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bioRxiv - Neuroscience 2020Quote: ... 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX; 10 μM; R&D Systems) was then added to determine the effect of AMPA receptor blockade on ePSCs ...
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bioRxiv - Microbiology 2022Quote: ... 100 uM of fluorogenic tripeptide substrate Methionine-Glycine-Proline-7-amido-methylcoumarin (R&D Systems ES017); and 2 ug/mL of MetAP enzyme (R&D Systems 3537-ZN) ...
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bioRxiv - Immunology 2022Quote: ... USA) and cultured for 7 days with 20 ng/ml recombinant M-CSF (R&D Systems) to allow them to differentiate to macrophages ...
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bioRxiv - Systems Biology 2023Quote: ... BMDMs were stimulated on day 7 with the appropriate doses of IL-6 (R&D Systems) and IL-10 (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.
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bioRxiv - Pathology 2022Quote: Serum KIM1 and NGAL concentrations were measured using mouse TIM-1/KIM-1/HAVCR and Lipocalin-2/NGAL quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... IFN-<ι>λ1/3 concentrations were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Blots were incubated for 1 hour with HRP-conjugated secondary antibodies diluted in 5% skim milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... and BSA (2%) and stained with following primary antibodies: PI16 (1:75, R&D Systems AF4929), GLUT1 (1:250 ...