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Citations for R&D Systems :
251 - 300 of 559 citations for 7 8 DIHYDROXY 4' METHOXYISOFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Microbiology 2022Quote: ... IL-4 (R&D Systems Cat#204-IL-050, 20ng/mL), αIgG (Agilent Cat#A042402-2) ...
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bioRxiv - Immunology 2021Quote: ... 10 ng/mL IL-4 from R&D Systems (404-ML), and 3 μg/mL anti-IFN-γ was added ...
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bioRxiv - Microbiology 2021Quote: ... 5 ng/mL BMP-4 (314-BP-050, R&D Systems), 50 ng/mL VEGF (293-VE-500 ...
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bioRxiv - Immunology 2020Quote: ... IL-4 and IL-13 were purchased from R&D Systems. Acid Phosphatase ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant 52-kDa pro-cath-D (4 µM; R&D Systems) was auto-activated to 51-kDa pseudo-cath-D in 0.1 M Na-acetate buffer (pH 3.5) ...
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bioRxiv - Biochemistry 2022Quote: ... 20 ng/mL bone morphogenetic protein 4 (BMP4, R&D Systems), and 10 ng/mL fibroblast growth factor-basic (bFGF ...
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bioRxiv - Immunology 2020Quote: ... 3 and 4 were measured using Luminex technology (R&D Systems), according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 ng human recombinant IGF2 (292-G2-050, R&D Systems) was used as positive control ...
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bioRxiv - Cell Biology 2021Quote: ... 30 ng/ml Bone morphogenic protein-4 (BMP4) (R&D systems), 1.5 μM CHIR99021 (Stemgent) ...
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bioRxiv - Microbiology 2020Quote: ... and 4,000 U/ml of rh IL-4 (R&D Systems) and cultured for 5 days ...
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bioRxiv - Neuroscience 2022Quote: ... or IL-4 (20 ng/mL, 24 h, R&D Systems). Following stimulation ...
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bioRxiv - Developmental Biology 2022Quote: ... BMP-4 (40 ng/ml, R&D Systems #5020-BP-010) or CHIR99021 (3 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... The caspase 4 inhibitor (Z-YVAD-FMK, FMK005, R&D systems) was used in further experiments at the dose of 50 µM ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µl of anti-V5-AF647 antibody (R&D Systems, FAB8926R) was used for staining cells for 20 minutes on a shaker at 500 rpm (Thermo Microplate Shaker) ...
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bioRxiv - Molecular Biology 2023Quote: ... and growth factors: 5 ng/ml BMP-4 (R&D Systems), 9 ng/ml Activin A (R&D Systems) ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 ng/mL human recombinant FGF-4 (R&D Systems). After 9 to 13 days ...
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bioRxiv - Immunology 2024Quote: ... TNFα and IL-4 using ELISA kits from R&D Systems in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and IL-4 (R&D Systems, Cat# 6507-IL-025/CF) in complete RPMI ...
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bioRxiv - Genomics 2019Quote: 8-week iEPs were stained with mouse E-cadherin-APC antibody (10 μL per one million cells, R&D Systems, FAB7481A) and sorted on a modified Beckman Coulter MoFlo into EcadHigh and EcadLow populations ...
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bioRxiv - Microbiology 2021Quote: ... IFNβ and IFNλ protein levels from HCT-8 cultures were measured by Human IFN-beta DuoSet ELISA (DY814, R&D Systems) and Human IL29/IL28B (IFN-lambda 1/3 ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Human IL-8/CXCL8 Quantikine ELISA kit and non-immune human IgG control were purchased from R&D Systems (Minneapolis, MN). Phorbol myristate acetate (PMA) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 12-well plates and treated for 8 hours in biological quadruplicates with IFNα (0.5 ng/ml, R&D Systems), IFNβ (0.5 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Microbiology 2019Quote: ... Mouse and human CXCL1 and CXCL2 as well as human IL-8 (CXCL8) were obtained from R&D Systems (Minneapolis, MN) and multiplex CXCL1 ...
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bioRxiv - Bioengineering 2020Quote: We used the method of Cell-based ELISA with using a Human Cleaved Caspase-8 (Asp391) Immunoassay kit (R&D systems).
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bioRxiv - Microbiology 2020Quote: ... Released IL-8 was determined in supernatants collected at 24 hrs using an ELISA kit (Biosource Europe and R&D Systems), according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... CRP and VDBP were conducted using Luminex® Magnetic Screening Assays (Catalogue number LXSAHM-8, R&D System, Minneapolis, MN, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2021Quote: Mice aged 8-10 weeks were given 0.75 μg/g body weight αCCL11 and αCCL24 (R&D Systems; MAB420 and MAB528) or an equivalent amount of rat IgG (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Developmental Biology 2022Quote: ... day 5 lateral plate mesoderm or day 7 differentiating lung mesenchyme was transferred to cSFDM supplemented with 100 ng/ml rmVEGF (R&D Systems, # 493-MV-005). For evaluation of differentiation efficiency ...
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bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Human mesenchymal stem cells (Passage 5-7) (commercially obtained from Rooster Bio (Frederick, MD)) were cultured in hMSC growth media (R&D Systems, Minneapolis, MN, USA). Adipose-derived stem cells (Passage 3-5 ...
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bioRxiv - Cell Biology 2020Quote: ... and then were seeded into upper chamber of transwell inserts (8 μM pore size, Costar) with or without coated Matrigel (R&D System). The lower chamber of the transwell was filled with 750 μl medium supplemented with 10% FBS which function as a chemoattractant ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Microbiology 2021Quote: Protein expression was analyzed by polyacrylamide gel electrophoresis on 8%-16% precast gradient gels (Thermo) and Western blotting using antibodies to ACE2 (AF933, R&D Systems), c-Myc-epitope (clone 9E10 ...
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bioRxiv - Cancer Biology 2019Quote: ... and the corresponding cryostat tissue sections (8-10μm thickness) were stained using the following primary antibodies: goat anti-CD31 (R&D System; AF3628), rabbit anti-caspase-3 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed EVs were concentrated with 3 kDa MWCO centrifugal filters and characterized via Luminex Multiplex Assay for TNF-α and IL-8 according to the manufacturer’s instructions (R&D Systems). Results were normalized by total protein content ...
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bioRxiv - Developmental Biology 2022Quote: Cells were dissociated with TrypLE on day 6 and 8 of CMEC and PMEC protocol and stained with VE-Cadherin PE-conjugated Antibody (R&D Systems). Then VEC+ cells were sorted using FACSAria III ...