Labshake search
Citations for R&D Systems :
251 - 300 of 667 citations for 6 9 Diazaspiro 4.5 decane 6 4 methoxyphenyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... condition media was collected and estimated quantity of the secretary IL-6 using commercially available ELISA kit (R&D Systems, DY 206-05) with human IL6 antibody (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Neuroscience 2023Quote: The IL-6 levels in astrocytes conditioned medium (ACM) were determined using the IL-6 ELISA kit per the manufacturer’s instruction (R&D Systems, Minneapolis, MN). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and hESCs were seeded at 3×105 cells/well onto 6-well plates and rested for 24hrs prior to stimulation with PBS or rhIL-23 (1290-IL-010, R&D Systems, USA) at concentrations of 1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Serum levels of cytokines (IL-6, TNF-α, MCP-1 and IL-10) were measured by corresponding ELISA kits (R&D Systems, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Mouse CXCL1/KC and interleukin-6 concentrations in lung homogenates from infected mice were determined using DuoSet® ELISA kits (R&D Systems, UK) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... offspring at postnatal day 3 to 6 received 7 intraperitoneal injections with either PBS (controls) or 75 ng of carrier free recombinant mouse interleukin-6 (rmIL6, R&D Systems, Minneapolis MN).
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bioRxiv - Cell Biology 2020Quote: ... IL-6 and TNFα cytokine levels in the media were then measured with DuoSet ELISA kits (R&D systems, catalog number DY406 and DY410) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... IL-6 was detected in culture supernatants from MSC/T cell co-culture assays by magnetic Luminex assays (R&D Systems, Biotechne, Abingdon, UK) as previously described [31] ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and 50 μg/ml L-Ascorbic Acid)) supplemented with Growth Factor Cocktail (5 ng/ml BMP4, 5 ng/ml bFGF, 10 ng/ml IL-6 (R&D systems, 206-IL), 10 ng/ml Flt3L (PeproTech ...
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bioRxiv - Immunology 2023Quote: Bronchoalveolar lavage fluid or conditioned culture media were collected for measurement of IL-6 with a Duoset ELISA kit (R&D Systems, DY406-05). Capture antibody was coated overnight in a 96 well plate at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... [9] and 100ng/ml ActivinA (R&D Systems, 338-AC-050) for 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL FGF9 (fibroblast growth factor 9; R&D Systems), and 1 μg/mL heparin (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: ... Antibodies included anti-MMP-9 (AF909; 1:100 R&D Systems); anti IL-1ß (ab9722 ...
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bioRxiv - Bioengineering 2023Quote: ... 200 ng/ml fibroblast growth factor 9 (FGF9; R&D Systems), and 1 μg/ml heparin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... containing 9 ng/mL Activin A (R&D systems, #338-AC), 5 ng/mL BMP4 (R&D systems ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Biochemistry 2022Quote: ... mice aged 6.5 weeks were starved for 6 hours and intraperitoneally injected with 220ng/g body weight of recombinant mouse FGF23 (2629-FG-025 R&D Systems, Minneapolis, MN, USA) in PBS-BSA 0.1% or PBS-BSA 0.1% vehicle with injection volume of 4μL/g body weight ...
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bioRxiv - Neuroscience 2022Quote: ... a complete media change was performed with CDMIII containing respective compounds at a final concentration of 8.8 ng/ml (422.6 nM) IL-6 (R&D Systems, Cat. no. 7270-IL-025) and 25 ng/ml (422.6 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the cells were plated at 0.2M cells/ml in a 6-well plate and stimulated with IFNβ (500 IU/ml, R&D systems cat. 8499-IF-010). At 24h ...
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bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... or MMP-9 at (1,10 & 100 ng/ml) (R&D Systems Europe) in protease buffer (50mM HEPES (pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... or caspase-9 inhibitors (50 μM Z-LEHD-FMK, R&D Systems), respectively ...
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bioRxiv - Immunology 2023Quote: ... moDCs were treated with recombinant galectin-9 protein (AF2045, R&D systems) at a final concentration of 1 µg/ml.
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bioRxiv - Molecular Biology 2023Quote: ... 9 ng/ml Recombinant Human/Mouse/rat Activin A (R&D Systems) and 5 ng/ml human FGF-2 (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
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bioRxiv - Immunology 2022Quote: ... plated at 1-2×104 cells/well in 96 U-bottom plate and cultured for 3 – 6 days in cRPMI medium in the presence of IL-33 (R&D Systems, #3626, 50 ng/ml) or TSLP (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... The sections were blocked with 5% skimmed milk in TBS-T at room temperature for 60 min and then incubated with rabbit anti-mMCP-6 antibody (R&D Systems, MN, USA, 1:500) at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: The conditioned media from treated RAW264.7s was used to quantify levels of multiple secreted cytokines/chemokines using their respective ELISA kits including IL-6 (DY406; R&D Systems Incorporated, Minneapolis, MN, USA), TNF-⍰ (DY410 ...
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bioRxiv - Cell Biology 2024Quote: ... TNF-α and IL-6 release was measured using DuoSet ELISA kits according to the manufacturer’s instructions (R&D Systems: DY417-05; DY410-05; DY406-05).
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bioRxiv - Physiology 2019Quote: ... and hyaluronan were measured using commercial enzyme-linked immunosorbent assay kits (IL-6: BioLegend, San Diego, CA, USA; corticosterone: Enzo Life Science Inc., Farmingdale, NY, USA; hyaluronan: R&D Systems, Minneapolis, MN, USA), according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... TNF-α (MTA00B) and IL-6 (M6000B) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits (ELISA Development System; R&D Systems, McKinley Place, MN, USA) following the manufacturer’s protocol.75
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bioRxiv - Developmental Biology 2024Quote: ... containing 40 mM DTT (ITW Reagents, Catalog No. A2948) or cultured in 2D on plates coated with 6 μg/ml laminin (R&D Systems, Catalog No. 3446-005-01). Cultures were done in TEC medium ...
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bioRxiv - Cell Biology 2019Quote: ... coverslips were subsequently incubated with anti-Galectin-9 antibody (AF23045, R&D systems) for 2 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... goat anti-human Galectin-9 at 20 μg/ml (AF23045, R&D systems); mouse monoclonal Rac1 (240106 ...
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bioRxiv - Neuroscience 2021Quote: ... and goat polyclonal antibodies anti-SOX-9 (1:40; AF3075; R&D Systems) overnight at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 9-cis retinoic acid and all-trans retinoic acid (both R&D Systems) were each dissolved in 100% DMSO ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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Potential of host serum protein biosignatures in the diagnosis of tuberculous meningitis in childrenbioRxiv - Immunology 2019Quote: ... ferritin and MMP-9 were assessed in Luminex kits purchased from R&D Systems Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stimulated with 50 ng/ml recombinant human BMP-9 (R&D Systems) and 150 ng/ml recombinant human VEGF (PeproTech ...