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Citations for R&D Systems :
2901 - 2950 of 3610 citations for Testosterone D5 98% 100 Ug Ml In Dioxane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin-streptomycin and 50 ng/mL recombinant human GM-CSF (R&D Systems 215-MC). M1 macrophage differentiation medium was prepared using M1 macrophage attachment medium supplemented with 100 ng/mL of LPS (Sigma-Aldrich L2630 ...
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bioRxiv - Cell Biology 2024Quote: ... Wnt3a was supplemented only on day 1 (25 ng/ml, R&D Systems, #5036-WN/CF). Thereafter ...
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bioRxiv - Cell Biology 2024Quote: ... FL5.12 cells were cultured in the presence of 10 ng/ml IL-3 (R&D Systems). Cell cycle exit in FL5.12 was achieved by washing the cells three times with PBS to remove IL-3 from the cells and by placing the cells in IL-3 free culture media for 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... The Phase I expansion media contains 50 ng/mL human stem cell factor (R&D Systems), 10 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Cancer Biology 2022Quote: Primary FACS-isolated mouse luminal and basal MECs were plated at a density of 5000 cells and 1000 cells respectively in ∼20uL of 100% rBM (R&D Systems, Cultrex BME) by pipetting around the edge of the well of a 96 well plate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
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bioRxiv - Developmental Biology 2023Quote: ... P0 were fixed and histologically sectioned and were stained with antibodies to detect Wnt4 (anti-Wnt4, R&D systems, cat# AF475, dilution 1:100), Wnt9b (anti-Wnt9b ...
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bioRxiv - Neuroscience 2020Quote: The primary antibodies used were Collagen IV (1:400 dilution; Cosmo Bio, Catalog Number LSL-LB1403) and CD13 (1:100 dilution; R&D Systems, Catalog Number AF2335). Mice were sacrificed by decapitation ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated with the primary antibodies (1:100 dilution; CD68, Bio-Rad, Cat# MCA1957; iNOS, Abcam, Cat# ab15323; CD206, R&D Systems, Cat# AF2535) in TBST containing 2% BSA for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Bioengineering 2024Quote: ... HLOs in Matrigel were either collected manually through pipetting in cold PBS-/- or isolated from Matrigel using Cultrex organoid harvesting solution (R&D Systems; 3700-100-01) according to the manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2024Quote: HLOs in Matrigel were either collected manually by pipetting in cold PBS-/- or isolated from Matrigel using Cultrex organoid harvesting solution (R&D Systems; 3700-100-01) according to the manufacturer’s recommended protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
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bioRxiv - Cell Biology 2023Quote: ... cells were transferred to wet ice and the remaining surface receptor was labeled with 1:100 anti-rabbit antibody conjugated to PE (F0110, R&D Systems, Minneapolis, MN, USA) in FACS buffer for 30 min on ice and protected from light ...
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bioRxiv - Developmental Biology 2023Quote: The following primary antibodies were used at indicated concentrations for the immunostaining of compresstome sections or iDisco whole lobe samples: goat anti-EPCR (R&D Systems, AF2749, 1:100), rat anti-Endomucin (Santa Cruz Biotech ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 2.5% BSA/0.1% Triton-X for 1 hour and subsequently incubated with an antibody cocktail consisting of goat anti-mouse PDGFRα (1:100) (catalog #: AF1062; R&D Systems; Minneapolis, MN, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Cancer Biology 2021Quote: ... Wild-type cultures were also supplemented with 500 ng/mL R-spondin (R&D Systems #3474-RS). For ex vivo growth assays ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in the presence of M-CSF (30 ng / mL; R&D Systems, Minneapolis, USA), and with RANKL (10 ng / mL ...
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bioRxiv - Immunology 2021Quote: ... the cells were stimulated with either 1 µg/ml recombinant murine CXCL13 (CXCL13, R&D Systems; Biotechne), 10 µg/ml AffiniPure F(ab’)2 fragment goat anti-mouse IgM (anti-IgM ...
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bioRxiv - Microbiology 2019Quote: ... L-glutamine (2mM) and 50 ng/mL of recombinant human M-CSF (R&D Systems, Minneapolis, Minnesota) for 6 to 7 days to differentiate them to macrophages [41] ...
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bioRxiv - Developmental Biology 2020Quote: ... or Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3) was placed in the upper and lower chambers ...
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bioRxiv - Neuroscience 2021Quote: ... The medium was supplemented with 20 ng/ml epidermal growth factor (EGF, #236-EG; R&D Systems) and 20 ng/ml basic fibroblast growth factor (bFGF ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and incubated with recombinant Jagged 1 protein (JAG1, 2 µg/ml, R&D System) for 3 h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... for 72 hours prior to stimulation with 10 ng/mL IFN-α (R&D Systems, 11101-2). Media was prepared by adding 120 μL of 10 mg/mL stock 13C11 15N2 L-tryptophan in 1x PBS to 240 mL of RPMI (Corning ...
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bioRxiv - Immunology 2019Quote: ... Following transfection cells were stimulated with 200 U/ml of recombinant human (h) TNFα (R&D Systems). Luciferase activity was assayed in cell lysates harvested 8 h post-stimulation ...
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bioRxiv - Physiology 2019Quote: ... fully differentiated mouse or human brown adipocytes were incubated with 100ng/ml activin B (R&D Systems) in DMEM/F12 supplemented with 10% FBS and 100µg/ml penicillin-streptomycin for 24h before lysis and for RNA extraction of RNA (as indicated below) ...
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bioRxiv - Immunology 2019Quote: ... which was either 20% autologous donor sera or 1 g/ml CXCL10 and CXCL12 (R&D Systems). Cell migration was assessed after incubation at 37°C for 4h ...
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bioRxiv - Bioengineering 2021Quote: ... Wt Ba/F3 cell lines were grown in media supplemented with 1ng/mL IL3 (R&D systems). Stable transductions were verified for puromycin resistance by using kill curve concentrations ranging from 0.25ug/mL to 2ug/mL.
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bioRxiv - Bioengineering 2020Quote: ... resuspended in complete culture media supplemented with 0.25 μg/mL neural growth factor (NGF, R&D Systems) and 0.1 μg/mL Ciliary Neurotrophic Factor (CNTF ...
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bioRxiv - Bioengineering 2020Quote: ... 2) transforming growth factor beta-2 (TGF-β2; 2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), 3 ...
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bioRxiv - Cell Biology 2021Quote: ... following 5 min of stimulation with 10 ng/mL PDGF-AA or PDGF-BB (R&D Systems) for the PDGFRα homodimer and PDGFRβ homodimer cell lines ...
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bioRxiv - Cell Biology 2021Quote: ... Osteoclast differentiation was induced by treatment with 25 ng/ml M-CSF (R&D Systems, Abingdon, UK) and 50 ng/ml RANKL (Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... inserts were coated for 2 hours with 2 μg/ml fibronectin (1918-FN-02M; R&D Systems) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The SOX2 primary antibody was used at 10 µg/mL (mouse anti-SOX2, R&D Systems #MAB2018).
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bioRxiv - Developmental Biology 2022Quote: ... the cells were exposed to 100ng/mL Activin A (R&D systems, Catalog# 338-AC-010/CF) and 3uM CHIR99021 (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... 1% penicillin/streptomycin (Cytiva, Marlborough, MA) and 20 ng/mL GM-CSF (R&D Systems, Minneapolis, MN) (DC media ...
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bioRxiv - Cell Biology 2022Quote: ... 24h later the medium was replaced by starvation medium containing 20ng/mL basic FGF (R&D Systems). After 72h the medium was removed ...
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bioRxiv - Biochemistry 2020Quote: ... and left unstimulated or stimulated with 10ng/ml FGF7 ligand (R&D Systems Cat#: 251-KG/CF) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... stimulated B cells were incubated with 20μg/ml anti–DC-SIGN mAb (clone 120507, R&D system) or mouse IgG (R & D Systems ...
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bioRxiv - Cancer Biology 2020Quote: idAdpgkG MC38 cells were treated with 20 ng/mL mouse interferon γ (mIFNγ, R&D Systems, Inc.) alone for 51 h prior to harvest (“control” condition) ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to that which contained 25 ng/ml mouse GM-CSF (R&D Systems). The primary microglia were harvested by shaking (200 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... and BMP4 (Sigma-Aldrich, Cat. H4916-10UG, R&D Systems, Cat. 314-BP-010/CF, 10ng/mL). In successive exposures ...
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bioRxiv - Immunology 2022Quote: ... either alone or in combination with anti-IFN-γ antibody (10 µg/ml, R&D Systems, MAB285) or NKG2D neutralizing antibody (5 µg/ml ...
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bioRxiv - Systems Biology 2020Quote: ... with 10% Fetal Bovine Serum and 100ng/ml recombinant Human M-CSF (R&D Systems; 216-MC) for 5 days ...
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bioRxiv - Molecular Biology 2019Quote: ... Differentiation toward germ layers was induced by addition of 50 ng/mL of BMP4 (R&D Systems) to mTeSR1 media ...