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Citations for R&D Systems :
2901 - 2950 of 3440 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... cultures were cotreated with 20 nM (2S,3S)-2-Amino-3-methyl-N-[2-(4-morpholinyl)ethyl]pentanamide dihydrochloride (LM11a-31)(R&D Systems, Minneapolis, MN, USA, Cat no: 5046) or vehicle solution consisting of DMSO during application of 6-OHDA ...
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bioRxiv - Developmental Biology 2021Quote: ... the medium was switched to FE-BM1 or BM2 containing 20 ng/ml recombinant human BMP4 (rhBMP4, R&D Systems), 50 nM retinoic acid (RA ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial suspensions were incubated with human SP-D at a concentration of 10mg/ml (R&D Systems, 1920-SP-050) for 1h at RT without agitation and observed by light microscopy (Nikon Ti Eclipse equipped with a metal-oxide-semiconductor (sCMOS ...
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bioRxiv - Neuroscience 2020Quote: ... During the first 10min, sections were superfused with either vehicle (i.e., 0.1%BSA/1xDPBS) or 100nM human SEMA3A Fc chimera (R&D System) diluted in warm (37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1 ...
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bioRxiv - Bioengineering 2020Quote: ... was used as an invasion promoter and recombinant human nodal (250 ng/mL; R&D Systems, 3218-ND-025/CF) was used as an invasion inhibitor ...
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bioRxiv - Immunology 2022Quote: ... The concentration of samples was extrapolated from standard curves of recombinant human IL7R-Fc chimera (R&D Systems, # 306-IR).
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bioRxiv - Biophysics 2022Quote: ... Recombinant VCAM-1/human Fc chimera protein was purchased from R&D Systems (R&D Systems Cat# 862-VC-100).
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bioRxiv - Cancer Biology 2022Quote: ... 104 sorted CD34+ HSPCs were plated for methylcellulose-based colony forming assays [Human Methylcellulose Base Medium HSC002 (R&D Systems), which were supplemented with 2% streptomycin/penicillin ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933 ...
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bioRxiv - Cancer Biology 2020Quote: ... Prostate-specific serum-free culture media contained 500 ng/mL human recombinant R-spondin (R&D Systems, Cat: 10820-904), 10uM SB202190 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718, RRID:AB_2278695; R&D Systems, Inc., Minneapolis, MN, USA); and developed using SuperSignalTM west -pico or -femto chemiluminescence kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-β1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng/ml human transforming growth factor-β3 (TGFβ3; cat. num. 243-B3-010/CF; R&D Systems, Minneapolis, MN), 1 µM Wnt-C59 ...
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bioRxiv - Immunology 2020Quote: ... and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems; Minneapolis, MN, USA) and 25 ng/mL recombinant human leukemia inhibitory factor (LIF ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Microbiology 2024Quote: Recombinant human IFN-β (8499-IF) and IFN-λ2 (1587-IL) were purchased from R&D Systems (Minneapolis, MN, USA). Both were reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were flooded with BrainPhys media with supplements and 10 ng/mL human NGF (256-GF-100, R&D Systems), and 3 μg/mL FRDU and incubated at 37° C and 5% CO2 until further experimentation.
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Biochemistry 2024Quote: The human cell line H1299 was acquired from ATCC and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant factors (murine FGF7, Peprotech, 450-60; human BMP3, Peprotech, 120-24B; rat CTGF, R&D Systems, 92-37C-T050) were resuspended in 0.1% BSA/PBS and used at final concentration 100 ng/mL.
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bioRxiv - Immunology 2023Quote: ... MDMs were stimulated in complete RPMI with either 10 ng/mL human IFN-ψ (285-IF-100, R&D Systems) or 50 ng/mL LPS ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were plated into semi-solid methylcellulose (MethoCult™ H4435 Enriched, STEMCELL Technologies or Enriched Human Methylcellulose, R&D Systems), with further addition of 100 pg/ml rh-IL21 ...
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bioRxiv - Developmental Biology 2023Quote: ... differentiation was induced by addition of human recombinant Activin A (ActA, 30 ng/ml, R&D systems, 338-AC-050).
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and florescent antibody for 15 minutes on ice: PB2 PE (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Immunology 2023Quote: ... Production of immune mediators in the supernatants was assessed using a human 45-plex (R&D Systems, Inc. Minneapolis, Minnesota).
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured with complete osteogenic medium mixed by human/mouse StemXVivo osteogenic/adipogenic base medium (CCM007, R&D Systems) and mouse StemXVivo ostegenic supplement (CCM009 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% FBS and Penicillin-Streptomycin) with or without 5 μg/ml recombinant human MANF protein (R&D systems, 3748-MN). For EdU cell proliferation assay and Ca2+ measurement ...
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bioRxiv - Bioengineering 2023Quote: ... Tissue constructs were cultured in the presence of 10ng/ml TGF-β3 (human recombinant active TGF-β3, R&D Systems) for the initial 14 days ...
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bioRxiv - Microbiology 2024Quote: ... each 25μl of pelleted resin was incubated with 2μg of recombinant human K63-linked (R&D systems, Cat# UC-320) or K48-linked (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293 cells stably expressing the human D1R (D1-HEK) were split into poly-L-lysine (R&D systems, Minneapolis, MN) coated 6-well plates at 450,000 cells per well ...
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bioRxiv - Cell Biology 2024Quote: ... then coated with indicated concentrations of recombinant human THSD7A overnight (diluted in sterile PBS) (R&D Systems Cat# 9524-TH). Cells were seeded onto THSD7A-coated coverslips and fixed and stained 24 hours post-seeding ...
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bioRxiv - Cell Biology 2024Quote: ... and treatments with 20ng/mL or 100 ng/mL recombinant human IL-13 protein (R&D Systems, 213-ILB-010). The supernatant and organoid lysates were collected to measure GLP-1 concentrations in secretion and total protein concentrations ...
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bioRxiv - Immunology 2024Quote: ... and probed at 4°C overnight with specific antibodies directed against human ACE2 (0.4 μg/mL; R&D Systems, MAB10823) or Mouse ACE2 (0.25 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... They were stained overnight at 4°C with mouse anti-human PLSCR1 monoclonal antibody (1:50 dilution, R&D Systems) and rabbit anti-human IFN-λR1 polyclonal antibody (1:100 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... Commercially available recombinant human ubiquitin mutants used in this study included (R&D Systems, UM-K48R, UM-K480, UM-NOK). L8A ubiquitin was purified as described previously 44 ...
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bioRxiv - Cell Biology 2024Quote: ... and human collagen I alpha 1 were assessed utilizing magnetic Luminex® Assays (R&D systems; #LXSARM-03 / #LXSAHM-04). The secretome samples used for these assays consisted of three samples from each culturing group that were pooled (n = 3) ...
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bioRxiv - Developmental Biology 2024Quote: ... micropat-terned hESC colonies were fixed in 4% paraformaldehyde and stained using anti-human beta-catenin antibody (R&D Systems) and secondary antibodies conjugated with Alexa Fluor 647.
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bioRxiv - Genomics 2021Quote: ... and incubated with antibodies against Kit (1:200 anti Kit AF1356 R&D Systems). The antigen-bound primary antibodies were detected using 1:500 dilution of biotinylated anti-goat IgG antibody (BA-5000-1.5 ...
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bioRxiv - Genomics 2021Quote: ... Kit protein was detected with 1:2000 anti c-kit (AF1356 R&D Systems) and 1:2000 of anti-goat secondary antibody (sc2020 ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IL-21 was from Preprotech (200-21) and IL-21 neutralizing antibody was from R&D Systems (991-R2).
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Neuroscience 2020Quote: ... or the indicated compounds were added at 12.5µM and incubated for 30 min at room temperature prior to adding biotinylated human VEGF-A165 (Cat#BT293, R&D systems) at 10 nM ...
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bioRxiv - Neuroscience 2021Quote: ... Sigma-Aldrich)-coated dishes in DMEM/F12/ B27/N2-medium supplemented with 200 ng/ml human recombinant sonic hedgehog (SHH, R&D Systems) and 100 ng/ml murine recombinant fibroblast growth factor 8b (FGF-8b ...