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Citations for R&D Systems :
2851 - 2900 of 3037 citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Immunology 2020Quote: Intestinal tissues from mice treated with PBS or spore for 7 d and 45 d were lysed with cell lysis buffer (R&D Systems) supplemented with 1% 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2019Quote: Protein levels in cell-free sputum samples of CXCL10/IP-10 and CCL5/RANTES were measured using duoset ELISA kits (R&D Systems), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... and Fc-ICAM-1 or Fc-VCAM-1 (Intercellular Adhesion Molecule 1; Vascular Cell Adhesion Molecule 1, R&D system, Minneapolis, MN) solution at 10 μg/mL overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Cell supernatants were collected and levels of IFNλ were determined with the IFN-lambda 1/3 DuoSet ELISA kit (R&D Systems). Plates were read on a Synergy HT plate reader (Biotek ...
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bioRxiv - Neuroscience 2019Quote: ... and cells were passaged as clumps every 5 days using ReLeSR (StemCell) in the presence of 5 μm ROCK inhibitor (R&D Systems). hiPSCs were characterized by positive IF staining of pluripotent stem cell markers OCT4 ...
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bioRxiv - Immunology 2020Quote: ... cell culture supernatants were collected and IL-8 secretion was determined using IL-8 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of TGFβ secreted by mouse pancreatic tumor cells was quantified by using the Mouse TGFβ 1 DuoSet ELISA (#DY1679-05, R&D systems), complemented with Sample Activation Kit 1 (#DY010 ...
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bioRxiv - Immunology 2020Quote: ... 5 × 105 cells in 100 μl adhesion buffer were added to the upper chamber and either 500 ng/ml CCL2 (R&D Systems) or 10 nM C5a (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
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bioRxiv - Immunology 2019Quote: ... normal T cell expressed and secreted (RANTES) and tumor necrosis factor alpha (TNF-α) by ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... or β-CATENIN cells were grown in Basal medium in 60 mm dishes and treated with either 50 ng/ml ActA (R&D systems) or with WNT3A/L-cell conditioned medium (L-CM)11 for 6h ...
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bioRxiv - Bioengineering 2019Quote: ... To induce differentiation FGF2 was withdrawn from the cell culture media and then replaced with bone morphogenic protein (BMP4; R&D Systems) or and Wnt3a (R&D Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated for 24 hours either with vehicle or with 10nM GnRH and 1nM activin A (R&D Systems, Minneapolis, MN) in serum-free DMEM with 0.1% BSA.
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bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were exposed to 1 µM EdU along with or withpout 2-100 ng/ml recombinant VEGFA (R&D Systems, 493-MV) for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with either M1-activation medium [RPMI160 medium with 20 ng/ml IFNγ (485-MI-100; R&D systems) and 10 ng/ml LPS (L2630 ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Developmental Biology 2020Quote: ... a brief wash with DMEM/F12 is followed with Day0 medium 30ng/ml Activin A (Cell Guidance Systems) + 40ng/ml BMP4 (R&D Systems) + 6uM CHIR99021 (Tocris ...
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 cells expressing dox-inducible shNT or shUGUUUGC shRNAs were mixed with 50% basement membrane extract (R&D Systems, Minneapolis, MN, USA) and injected subcutaneously into the flanks (3×106 cell per injection) ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Immunology 2022Quote: ... CXCL10 was measured in plasma and cell supernatant using a DuoSet ELISA Kit (R&D Systems DY466-05, R&D Systems DY008B). ELISAs were performed according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... CXCL10 was measured in plasma and cell supernatant using a DuoSet ELISA Kit (R&D Systems DY466-05, R&D Systems DY008B). ELISAs were performed according to manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: The location of DC-SIGN was assessed at the surface of BHK-21 cells (not permeabilized) by flow cytometry using an anti-DC-SIGN phycoerythrin-conjugated antibody (FAB1621P R&D Systems) according to a standard procedure (Lozach et al. ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were minced and digested with collagenase to single cells before embedded in reduced growth factor extracellular matrix (Cultrex BME2, R&D Systems). Selective isolation of ductal stem cells was achieved using Isolation Medium (IM ...
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bioRxiv - Neuroscience 2022Quote: ... We used 250 µg of cell lysates for each of the parts A and B of the array and followed manufacturers’ instructions (R&D Systems).
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated in cSFDM (including B27 supplement with vitamin A) with 200 ng/ml rmWNT3A (R&D Systems, #1324-WN-010), 100 ng/ml rhFGF2 (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: ... the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 µg/ml, R&D Systems, MAB17781) for 1h at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Physiology 2024Quote: CXCL5/LIX in culture media of WT and TLR3-/- endothelial cells were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturers’ instructions (R&D Systems).
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bioRxiv - Molecular Biology 2024Quote: Total CCL2 and CCL17 concentration in the enriched cell free BALF was calculated using mouse CCL2 and CCL17 DuoSet Elisa (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... NK cells were also co-cultured with K562 or P815 coated with anti-NCR (NKp30, NKp44, NKp46, R&D Systems, 5ug/ml) or anti-CD16 (clone 3G8 ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... cells were filtered through a screen and washed with 5 mM EDTA in EHAA + 2.5% FBS (R&D Systems, Cat. No.: S12450). The 8 popliteal LNs digested for dendritic cells isolation were then stained with CD11c (N418) ...
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bioRxiv - Biophysics 2024Quote: Recombinant sKLA (Glu34-Ser981) with a C-terminal 6His tag expressed in a NS0 murine myeloma cell line was purchased (R&D Systems) and loaded onto a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with affinity-purified polyclonal goat anti-huACE2 antibody recognizing the extracellular domain (1:40 dilution, #AF933, R&D Systems) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: Culture media of cells infected with virus were collected at the indicated times and used for ELISA quantification of IFNβ (R&D Systems) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Immunology 2023Quote: ... naïve B cells or CH12F3 cells were treated with 10ng/ml F(ab’)2 Fragment Goat Anti-Mouse IgM or 10ng/ml mIL-5 (R&D Systems) for 5 or 10 min and then collected for Western blotting or RNA extraction.