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Citations for R&D Systems :
2851 - 2900 of 3035 citations for Anti USP30 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: pH 9.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), SOX9 (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: PB2 PE (phycoerythrin) (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Cell Biology 2023Quote: ... All sections were incubated with a biotinylated secondary antibody and stained using the R&D HRP-DAB staining kit (R&D Systems, USA) and then counterstained with hematoxylin ...
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bioRxiv - Cell Biology 2023Quote: ... and tissue harvest as previously described and in Supplementary Methods.6 Structural analysis of lung tissue included hematoxylin and eosin stain as well as immunostain for muscularization of vessels as previously described.7 Cytokine levels were measured by pooling plasma and lung lysate from 5 mice treated with either L-NAME/HFD or control water/diet for 5 weeks and was assayed using an antibody-based cytokine array (R&D Systems, ARY006) and was completed according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3; mSPARCL1 1:500, R&D systems, #AF2836-SP; hSPARCL1 1:500, R&D systems, #AF2728-SP ...
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bioRxiv - Cancer Biology 2023Quote: ... Lymphatics were visualized by injecting 0.4ug of Mouse LYVE-1 Alexa Fluor® 594-conjugated Antibody (20ul of concentration 20ug/ml, diluted 1:10 in normal saline; cat# FAB2125T; R&D systems) into the tongue in a region near the tumor 20 minutes before imaging ...
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bioRxiv - Cell Biology 2023Quote: ... plasma and tissues of interest were collected at different post-infection times and assessed by ELISA using commercially available antibodies and according to the procedures supplied by the manufacturer (R&D Systems, Minneapolis). All samples were stored at -20°C until processing ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with horseradish peroxidase-coupled anti-mouse immunoglobulin (R&D System, Minneapolis, MN, USA) at room temperature for 1 h and after subsequent washes ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with horseradish peroxidase-coupled anti-mouse immunoglobulin (R&D System, Minneapolis, MN, USA) at room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Neuroscience 2020Quote: ... 4) incubation with a donkey anti-rabbit IgG conjugated with NorthernLights™ NL557 (#NL004, R&D System) diluted 1:1000 for 2 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Microbiology 2019Quote: ... purified donkey anti-goat IgG pAb (#AF109) and monoclonal mouse IgG (#MAB002) were from R&D Systems. Purified rabbit anti-TIM4 pAb (#ab47637 ...
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor® 647-conjugated anti-human lymphatic vessel endothelial receptor-1 (LYVE-1 ,R&D Systems) or Alexa Fluor™ 488-conjugated anti-human laminin (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... stimulated B cells were incubated with 20μg/ml anti–DC-SIGN mAb (clone 120507, R&D system) or mouse IgG (R & D Systems ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-platelet derived growth factor receptor-α (PDGFRα, 1:200, AF1062. R&D Systems, MN, USA), goat anti-Iba1 (1:200 ...
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bioRxiv - Pathology 2020Quote: ... goat polyclonal anti YM1/Chitinase-3 like 3 (R&D systems, Cat. No. AF2446, 1:400 dilution). Antigen retrieval of HIER2 (EDTA ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were sorted for LRP6 knockdown using flow cytometry (anti-human LRP6-APC, R&D Systems MAB1505) and successful knockdown was confirmed by Western blot.
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bioRxiv - Physiology 2021Quote: ... Human MPCs were trypsinized and incubated 30 min with biotinylated anti-human PDGFRα (BAF322 R&D Systems) goat polyclonal antibody and CD56-PE (clone B159 ...
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bioRxiv - Molecular Biology 2021Quote: ... was obtained from Cell Signaling (Frankfurt am Main, Germany) and goat anti-mouse Il-36α (AF2297, RRID:AB_355216) was from R&D Systems/Bio-Techne (Wiesbaden-Nordenstadt ...
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bioRxiv - Genetics 2020Quote: ... sections were incubated with 5 μg/ml goat anti-mouse CD31 (R&D Systems, Inc, Minneapolis, MN) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The anti-DC-SIGN phycoerythrin-conjugated fragment antigen binding (Fab) 1621P was purchased from R&D Systems. Soluble C6-GlcCer was purchased from Cayman Chemical and dissolved in methanol ...
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bioRxiv - Pathology 2024Quote: ... Cells were then immunolabeled for 4hr at 4°C with sheep anti-CD31 (R&D Systems, AF806) + rabbit anti-cTNT (Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... and anti-mouse CD142 (Sino Biological, 50413-R001, 1:100; or R&D Systems, AF3178, 1:50). Anti-mouse CD142 antibodies were conjugated with Biotium Mix-n-Stain CF647 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... and Mouse Aminopeptidase N/CD13 (Goat anti-CD13, R&D Systems, cat. no.: AF2335, dilution: 1:100). Primary antibodies were incubated for 10 days at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Cryosections were stained with a monoclonal rat IgG2a anti-mouse NKp46/NCR1 (R&D Systems, clone # 29A1.4) and revealed using a donkey AF568-conjugated anti-rat pAb (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... and prepared for GPC6 staining (goat anti-mouse Gpc6, 10µg/mL, R&D Systems, cn: AF1053, ln:GJA0219031) through the following treatment ...
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bioRxiv - Genomics 2023Quote: ... Precleared lysates were incubated with 1 mg goat anti-EPHB4 polyclonal IgG (R&D systems, AF446-SP) at 4°C for 3 hours followed by addition of 10 ml Protein A Dynabeads (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Anti-TRAIL ELISA was performed using a Human TRAIL/TNFSF10 Quantikine ELISA Kit (R&D Systems, DTRL00) following manufacturer-provided protocols ...
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bioRxiv - Immunology 2023Quote: ... Membranes were probed using goat anti-mouse LDLR at a 1:500 dilution (AF2255; R&D Systems) or goat anti-human LDLR at a 1:500 dilution (AF2148 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Pathology 2021Quote: Formalin-fixed and paraffin-embedded liver sections were subjected to a standard procedure of immunohistochemistry with primary antibodies against F4/80 (eBioscience, San Diego, CA) and myeloperoxidase (MPO, R&D System, Minneapolis, MN). The liver sections were additionally subjected to Masson’s trichrome staining ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
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bioRxiv - Cell Biology 2020Quote: Conditioned media of papillary and reticular fibroblasts from the three donors were analysed using human angiogenesis antibody array (R&D systems, Minneapolis MN, USA). Images were acquired using Fusion Capt Advance FX7 (Vilber ...
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bioRxiv - Cancer Biology 2021Quote: ... Then membranes were blocked in 5% non-fat milk in TBS-T for 30 minutes at room temperature and incubated with primary antibodies against Robo1 (R&D Systems, 1:500), Robo2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS or conditioned media from lung stromal cells from HFD-fed mice treated with 5 μg/mL of either CSF2 neutralizing antibodies (R&D Systems, MAB415-100) or rat IgG antibodies (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Immunology 2021Quote: ... Immunostaining nanoparticle tracking analysis (NTA) was performed using fluorescently labeled antibody conjugate directed against human CD63 (AlexaFluor488-conjugated clone 460305; R&D Systems (Minneapolis, USA)) ...
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bioRxiv - Immunology 2021Quote: Multiplexed ELISA on interstitial fluid from matched T and NAT fresh tissues were performed on a Luminex 200 platform (Luminex Inc.,) using custom kits of pre-mixed antibody-coated beads (R&D System Inc., MN), which included the following analytes ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using goat-α-Shh antibodies (R&D Systems, AF464), rabbit-α-GAPDH antibodies (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... the top ring (as viewed in Figure 2d) on each waveguide was spotted with mouse IgG1 isotype control antibody (R&D Systems, Minneapolis, MN) at 1000 μg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Microbiology 2024Quote: Macrophage supernatants were thawed on ice and assayed for the presence of 40 cytokines using Proteome Profiler Antibody Arrays (R&D Systems, Minneapolis, MN). Procedures were followed according to manufacturer’s instructions ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...