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Citations for R&D Systems :
2701 - 2750 of 4230 citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and incubated with one of the following primary antibodies: goat anti-Cyclin D1/D2 (R&D Systems, cat: AF4196), rabbit anti-Phosphorylated Cyclin D-Thr286 (cell signaling ...
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used for Western Blotting: goat anti-Galectin-9 (AF2045, R&D systems, Minneapolis, Minnesota) at 1:1000 (v/v) ...
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bioRxiv - Molecular Biology 2024Quote: ... Antigen-retrieved sections were incubated overnight at 4 °C with goat anti-CD45 antibody (R&D Systems, AF114-SP) and rabbit anti-CD68 antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of active MMP9 on 200μg of total proteins lysate was quantified by using a Human Active MMP-9 Fluorokine E assay kit (F9M00; R&D Systems, Noyal Châtillon sur Seiche, France) according to the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... For ICAM-1 cross-linking experiments on ECs we used mouse anti ICAM-1 (Clone # BBIG-I1) from R&D systems and secondary antibody from donkey (DK) ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibody incubation was done overnight at 4 °C with following dilutions: anti-PLOD2 mouse at 1:500-1:1,000 (MAB4445, R&D Systems), anti-FERMT3 mouse at 1:500-1:1,500 (ab173416 ...
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bioRxiv - Cell Biology 2021Quote: ... Serum IGF-1 levels were measured by Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA). Serum parameters were measured and calculated with a VITROS analyzer (Ortho Clinical Diagnostics ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-beta 1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Physiology 2023Quote: ... for the measurement of plasma VEGF-A and sFlt-1(Mouse VEGFR1/Flt-1 Quantikine ELISA Kit, R&D Systems, USA) levels ...
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bioRxiv - Physiology 2024Quote: ... supernatants were collected and used to measure IGF-1 levels by Mouse/Rat IGF-1 Quantikine ELISA (R&D systems, MG100). BCA assay was performed to normalize IGF-1 concentration to protein concentration in the liver ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with PE-conjugated anti-CD140b antibody (2.5 μg/ml, PR7212 clone, Mouse IgG1, R&D Systems) and FITC-conjugated anti-CD146 antibodies (5 μg/ml ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... and immunostained with 0.5 μg/ml mouse monoclonal anti-myosin heavy chain (MHC) antibody (MF20; R&D Systems, MN, USA) and 1.0 μg/ml rabbit polyclonal anti-nucleolin antibody (ab22758 ...
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bioRxiv - Bioengineering 2021Quote: ... 12 million cells were purified for CD90+ cells using a PE-conjugated antibody for anti-CD90 (R&D Systems, FAB2067P), the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2020Quote: C57BL6/J male mice (#000664) received an intraperitoneal injection of 45μg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... and immunostained with 0.5 μg/ml mouse monoclonal anti-myosin heavy chain (MHC) antibody (MF20; R&D Systems, MN, USA). 0.1 μg/ml of Alexa Fluor 488-conjugated donkey polyclonal anti-mouse IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2021Quote: ... or rabbit IgG isotype control followed by staining with APC-conjugated anti-rabbit IgG secondary antibody (F0111, R&D systems).
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bioRxiv - Cell Biology 2022Quote: Cells were characterised using hPSC imaging kit (Human Pluripotent Stem Cell Marker Antibody Panel kit, SC008, R&D system, UK) with analysis performed on a flow cytometer (Beckman Coulter Cytomics FC 500) ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Immunology 2021Quote: ... The cocktail included 10 μg/mL each of a mouse anti-CD63 monoclonal antibody (#MAB5048, R&D Systems, Minneapolis, MN) and a mouse anti-CD9 monoclonal antibody (#MAB1880 ...
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bioRxiv - Cell Biology 2020Quote: ... and immunostained with 0.5 μg/ml mouse monoclonal anti-myosin heavy chain (MHC) antibody (MF20; R&D Systems, MN, USA) and 1.0 μg/ml rabbit polyclonal anti-nucleolin antibody (ab22758 ...
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bioRxiv - Bioengineering 2022Quote: ... The tissue section slides were fixed and stained with a rat anti-intelectin monoclonal antibody (R&D Systems, Clone 746420) with fluorescent detection and DAPI (ProLong antifade reagent with DAPI) ...
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bioRxiv - Cancer Biology 2024Quote: The phosphorylation profiles of multiple effector kinases were screened using a phospho-kinase antibody array (R&D System, cat. #ARY003B). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... The following antibodies were used for immuno-isolation of NRXN3 and controls: sheep polyclonal NRXN3 (cat. AF5269, R&D Systems), GFP (4745-1051 ...
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bioRxiv - Genetics 2024Quote: ... For immunoprecipitation 1mg of pre-cleaned cell lysate was incubated with 4µg mouse monoclonal L1TD1 antibody (R&D systems, MAB8317) at 4 °C at 20rpm on the rotor overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5% bovine serum albumin (BSA)) and labeled with 0.02 µg/well un-conjugated anti-ACKR3 antibody (11G8, R&D Systems) for 1 hour at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and florescent antibody for 15 minutes on ice: PB2 PE (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Cancer Biology 2024Quote: Western blots were performed as previously described.31 Primary antibodies used include Mb (catalog G-125-C, R&D Systems), CPT1a (catalog 97361 ...
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bioRxiv - Molecular Biology 2024Quote: ... PKR was then immunoprecipitated from 100 ug of this lysate using anti-PKR monoclonal antibody (R&D Systems Technology: MAB1980) in a high salt buffer (20 mM Tris–HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Positive control neutrophils were incorporated into MCDB-131 Complete Media at a concentration of 900k cells.mL-1 and supplemented with 10 ng.mL-1 IL-8 (rh CXCL8/IL-8, R&D Systems, Minneapolis, MN) for 15 minutes before rinsing and stained ...
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bioRxiv - Pathology 2022Quote: Serum KIM1 and NGAL concentrations were measured using mouse TIM-1/KIM-1/HAVCR and Lipocalin-2/NGAL quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... cells were refreshed in DMEM + 1% (v/v) PS + 1% (v/v) B-27 + 50 ng/ml EGF (Cat. No. 236-EG, R&D System) + 25 ng/ml FGF7 for 4 days ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Immunology 2022Quote: ... 1×106 PMBCs were incubated with 20 ng of MIP-1β (recombinant human CCL4L1/MIP-1β isoform LAG-1; R&D Systems) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Neuroscience 2024Quote: ... onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM, R&D systems). To monitor and ensure the genetic stability of the cells ...
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Microbiology 2023Quote: ... for 48 hours at which point the media was collected and analyzed for PAI-1 content using the Human Serpin E1/PAI-1 Quantikine ELISA Kit (DSE100, R&D Systems) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
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bioRxiv - Microbiology 2024Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1(R&D systems, Cat# E-522B), 2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... bEFNB2 expressing CHO cells were passaged in puromycin (1-5 ug/mL) for 1 week and then stained with EphB3-Fc (5667-B3-050; R&D Systems), a natural ligand of EFNB2/3 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
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bioRxiv - Cell Biology 2020Quote: ... α-fetoprotein (R&D Systems, MAB1368, diluted at 1/100), and CYP3A4 (SANTA CRUZ BIOTECHNOLOGY ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-Klf5 (1:500, R&D Systems, AF3758), rabbit anti-Cldn1 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Sox10 (1:800, R&D Systems, MAB2864), rabbit anti-Sox9 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-GAPDH (1:10,000, #MAB5718, R&D Systems)