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Citations for R&D Systems :
2601 - 2650 of 3410 citations for Mouse NLR Family CARD Domain Containing 4 NLRC4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated with goat anti-mouse CD31 primary antibodies (R&D Systems, #AF3628; 1:100) in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... collected hydrogels from the device were stained with Human/Mouse E-Cadherin Antibody (R&D Systems) at a final concentration of 5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-rabbit Nucleocapsid (NovusBiologics ...
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bioRxiv - Biochemistry 2023Quote: ... Monoclonal mouse anti-human ACE2 Antibody (MAB933) was purchased from R&D Systems (Minneapolis, MN, U.S.A.). VE-cadherin extracellular domain (EC1-5 ...
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bioRxiv - Systems Biology 2023Quote: ... and 20 mM Hepes supplemented with recombinant mouse M-CSF (60 ng/ml; R&D Systems). BMDMs were scraped with cold PBS and plated in either a 48 or 96 well plate for qPCR and IF ...
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bioRxiv - Immunology 2023Quote: ... probed with anti-mouse Ms-RD-SARS-COV-2 (R&D Systems, MAB105401, 1:10 dilution) or anti-mouse H3 (Immune-Tech ...
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bioRxiv - Biochemistry 2023Quote: ... and 10 ng/mL recombinant mouse interferon-γ (IFN-γ) (R&D Systems, 485-MI-100) were added to the media and maintained with subsequent media change.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Circulating chemokines levels were analyzed via the Proteome Profiler Mouse Cytokine Array from R&D System. Serum was pooled from six CCR5+/+ mice treated with vehicle or aldosterone ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were incubated overnight with mouse anti-gp130 (R&D systems, Minneapolis, MN, USA, clone 29104), rabbit anti-pSTAT3-Y705 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... and Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody (1:100, NL009, R&D system) respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against human DDR1 (R&D Systems, AF2396), CXCL5 (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... Macrophage M2 polarization was obtained by incubation with 20 ng/ml of IL-4 (R&D Systems, #204-IL) and 20 ng/ml of IL-13 (R&D Systems ...
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bioRxiv - Bioengineering 2019Quote: ... and 0.005 N NaOH along with 10 ng/mL bone morphogenetic protein 4 (R&D Systems, Minneapolis, MN, USA). 4 × 105 cells were plated in each PDMS culture well with 3-mm pillar ...
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bioRxiv - Physiology 2020Quote: ... one multiplex panel was used to measure fatty acid binding protein 4 (FABP4) and resistin (R&D Systems, UK); non-esterified fatty acid (NEFA ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour at 4°C and the supernatant was precipitated with antibodies against BACH1 (AF5776, R&D System), or IgG (normal mouse IgG ...
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bioRxiv - Immunology 2020Quote: ... AA-Mφ were generated by stimulating macrophages overnight with 20 ng/ml IL-4 (R&D Systems, Minneapolis, MN). Reg-Mφ were generated by stimulating macrophages overnight with 100 ng/ml LPS and either 200 nM PGE2 (Cayman Chemical ...
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bioRxiv - Molecular Biology 2022Quote: ... CH12F3-2 cells were incubated in culture medium supplemented with10ng/mL IL-4 (R&D Systems #404-ML-050), 1ng/mL TGFβ (R&D Systems #7666-MB-005 ...
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bioRxiv - Immunology 2023Quote: ... a 50:50 mixture of Matrigel Matrix GF and 4 mg/ml Cultrex Rat Collagen I (R&D Systems). For time-lapse live imaging experiments ...
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bioRxiv - Immunology 2023Quote: ... were diluted to 4 µg/ml in PBS along with 1 µg/ml VCAM-1-Fc (R&D Systems). To coat ...
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bioRxiv - Neuroscience 2023Quote: ... Specified primary antibodies were incubated over-night at 4°C: goat anti-ICAM1 (1:400, R&D systems AF796), rat anti-cluster differentiation 31 (CD31 ...
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bioRxiv - Molecular Biology 2024Quote: ... Antigen-retrieved sections were incubated overnight at 4 °C with goat anti-CD45 antibody (R&D Systems, AF114-SP) and rabbit anti-CD68 antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 50 ng/mL human bone morphogenetic protein 4 (hBMP4) (R&D Systems, Minneapolis, Minnesota, USA), 50 ng/mL human vascular endothelial growth factor (hVEGF ...
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bioRxiv - Cell Biology 2021Quote: The Proteome Profiler Human Phospho-RTK array kit and human phospho-kinase antibody array kit (R&D Systems) were used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were converted to an epiblast-like (EpiLC) state by 48 hour culture in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Neuroscience 2019Quote: ... Cells incubated at 37°C/5%CO2 for approximately 1hr prior to flooding the dishes with F12 complete media alone or complete F12 media containing 100ng/mL GDNF (R&D Systems). Cells incubated overnight at 37°C/5%CO2 prior to being rinsed in PB and fixed in 3% paraformaldehyde ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Immunology 2019Quote: ... a suspension of 106 mononuclear cells in RPMI 1640 supplemented with 10% FCS was added to each insert in a well containing a solution of chemokine (R&D Systems). In some experiments ...
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bioRxiv - Cell Biology 2021Quote: ... and inserts were immersed in 500 µL RPMI media containing 10% FBS in the absence or presence of 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for 24 h ...
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bioRxiv - Systems Biology 2021Quote: ... washed once with FACS buffer (1%BSA in PBS and 5mM EDTA) and incubated for 40 minutes in FACS buffer containing 0.75ug of human ACE2-488 conjugated antibody (R&D Systems FAB9332) and 1:1000 7-AAD (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Immunology 2021Quote: Mature macrophages (n = 6 donors) were stimulated for 48 hours with complete medium containing IFN-γ (20 ng/mL, R&D Systems) and LPS (100 ng/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant proteins were separately preincubated with neuronal cultures and recombinant tau protein for 3 hours prior to incubation of neuronal cultures with the mixer containing recombinant proteins (100nM Human LRP-1 Cluster IV Fc Chimera Protein; 10nM Human LRPAP Protein [R&D systems]). For pharmacological treatments ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
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bioRxiv - Immunology 2020Quote: ... and then re-suspended in 30 μL of media containing 10,000 units/mL of recombinant IFN-α2a or 100 units/mL of recombinant IFN-γ (R&D Systems). Antibodies against SiglecF ...
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bioRxiv - Cell Biology 2021Quote: ... Cells in ATP depletion were prepared by culturing the cells in a medium containing 100 μM CCCP (R&D systems, Minneapolis, MN) and 600 μM 2-deoxy-D-glucose (2DG ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Cell Biology 2021Quote: LECs were pre-cultured with FBS-free and supplement-free basal medium for 24 h and then treated with medium containing 100 ng/mL of recombinant rat CTGF (R&D Systems) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: Healthy dermal fibroblasts were serum starved for 24 h in DMEM containing 0.5% FBS and stimulated with 10 ng/ml TGF-β (R&D systems), 100 ng/ml Wnt-3a (R&D systems ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...
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bioRxiv - Cell Biology 2022Quote: ... media was replaced with fresh media containing MCSF and receptor activator of nuclear κB (RANKL) (25 ng/mL each, R&D Systems), and media refreshed every 2-3 days thereafter until a total of seven days stimulation with RANKL ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 0.1% FBS (Hyclone Laboratories Inc.)] and stimulated with 10 ng/mL rat PDGF-AA ligand (R&D Systems, Minneapolis, MN, USA) diluted from a 1.5 μg/mL working solution in 40 nM HCl containing 0.1% BSA for the indicated length of time ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Microbiology 2024Quote: ... This medium was aspirated and replaced with complete medium containing 20 μg/ml gentamicin and IFNβ (R&D Systems, 8234-MB-010) as indicated for 20 hours ...
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...