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Citations for R&D Systems :
2601 - 2650 of 6141 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: CXCL12 concentration in supernatants of CXCL12 Ch/DS NC was quantified using Mouse CXCL12 DuoSet ELISA as per supplier instructions (R&D systems, Minneapolis, USA). Encapsulation efficiency and drug loading were calculated as follow:
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bioRxiv - Cancer Biology 2022Quote: The levels of murine TRAIL/TNFSF10 (DY1121) in the cells were measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (R&D Systems, Emoriville, California, USA). The OD value was measured by Microplate Reader (Multiskan Spectrum ...
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
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bioRxiv - Immunology 2020Quote: Secreted IL-1b and MCP-1 proteins in supernatants of monocytic cells stimulated with TNF-α were quantified using sandwich ELISA following the manufacturer’s instructions (R&D systems, Minneapolis, USA).
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bioRxiv - Immunology 2019Quote: ... and the tissue levels of myeloperoxidase (MPO) in the supernatants were determined using a mouse MPO ELISA (R&D systems, Biotechne, Minneapolis, MN, USA). The fixed tissues (left lungs ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Neuroscience 2022Quote: ... we determined the amount of HB-EGF per cell through enzyme linked immunosorbent assay (ELISA; Cat# DY8239-05, R&D Systems, Minneapolis, MN, USA). For analysis of HB-EGF levels in brain tissue ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and WNT16 levels were determined in senescent fibroblast lysates using a standardized sandwich Enzyme-Linked Immunoassay (ELISA) technique (R&D Systems, Minneapolis, MN, USA), according to manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants or sulcus fluid from patients with different disease stage were evaluated for MMP-9 content using ELISA Duo Set (DY911, R&D System, Wiesbaden, Germany.) following the manufactureŕs protocol.
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested to measure the release of IFN-γ using a IFN-γ-specific ELISA according to the manufacturer’s instructions (R&D Systems, DY28B05). To evaluate T cell activation markers ...
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bioRxiv - Physiology 2024Quote: ... 50 µL of murine plasma was then used to quantify glucagon concentrations with ELISA by following the manufacturer’s protocol (R&D Systems, cat no. DGCG0). Final glucagon concentrations were calculated based on a standard curve.
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bioRxiv - Molecular Biology 2024Quote: ... The secretion of IFN-γ was also quantified in cell supernatants using a sandwich mouse IFN-γ Duo Set ELISA (DY485-05, R&D systems). Standards and samples were diluted appropriately to obtain results within the stated detection levels.
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged and resuspended in mTeSR1 supplemented with 10 μM SB431542 (SB; Stemgent) and 1 μM Dorsomorphin (R&D Systems). 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm ...
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bioRxiv - Microbiology 2019Quote: ... using known concentrations of commercial rBoIFN-γ (20, 15, 10, 5, 3 and 1 ng; 2300-BG-025, R&D Systems).
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bioRxiv - Neuroscience 2020Quote: ... OPCs were treated with LCN2 (1, 2.5, 5, 10, 20, 40, 80, or 160 ng/ml, R&D Systems, Shanghai, China) or vehicle control (0.0002% BSA ...
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking in 10% donkey serum was followed by overnight incubation at 4°C with anti-CD31 (1:100; R&D Systems) and subsequent incubation with a donkey anti-goat AlexFluor594 secondary antibody (1:500 ...
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bioRxiv - Genomics 2022Quote: ... samples were thawed and cleared by centrifugation for 5 minutes at 1000xg and assayed for urine MMP7 and MMP9 by ELISA according to manufacturer’s instructions (R&D Systems, DMP700/DMP900, Minneapolis, MN). Urine creatinine was used to normalize MMP7 and MMP9 levels between non-active ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Cell Biology 2019Quote: ... 5.0 × 105 PCs and FBs were grown in a single well of a 24-well dish for 24 hours and enzyme-linked immunosorbent assay (ELISA) was performed per manufacture protocol (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Pathology 2020Quote: ... TNF-α and IL-1β) in the frozen distal small intestine and colon were measured by porcine specific ELISA assays (R&D Systems, Abingdon, UK).
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bioRxiv - Physiology 2019Quote: ... the clones were assayed for IFN-γ secretion in a peptide-specific manner following their co-incubation with T2 cells pulsed with MART-126–35 (commercially synthesized and purified [>95%] by reverse-phase HPLC by Biomer Technology, Cheshire, UK) by ELISA (R&D Systems, Minneapolis, MN, USA). The MART-126–35–-reactive clones were further expanded in a second-round of exposure to 30 ng/ml anti-CD3 ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected 24 hours later and stored at -80 °C until analysis of TNF-⍰ levels via ELISA (DY210; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with recombinant human IFNγ (R&D Systems, 285-IF-100) by adding an equal volume of the complete culture media containing the cytokine ...
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bioRxiv - Cell Biology 2020Quote: ... Gas1 was stained using goat anti-human Gas1 antibodies (R&D systems, Cat# AF2636). Dhh was stained using mouse anti-Dhh antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human TGF-β (7754-BH) was purchased from R&D Systems (Minneapolis, MI). It was reconstituted at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used: Human alpha-Smooth Muscle Actin (R&D Systems, MAB1420), Human/Mouse alpha-Fetoprotein/AFP (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... during 2 hours and stimulated with human recombinant VEGF165 (25ng/ml, R&D System) during 30 minutes.
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bioRxiv - Genomics 2019Quote: ... 50 ng/mL carrier-free recombinant human stem cell factor (SCF; R&D Systems), 50 ng/mL TPO (R&D Systems) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 100ng/mL human recombinant FGF4 (R&D Systems, 235-F4) in the presence of 10 μg/mL heparin sulfate and 3 μM BI-D1870 RSK inhibitor at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... PDGF-AB were determined by Human Magnetic Luminex Assays (R&D Systems, Minneapolis, MN) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... NMPM: N2B27 supplemented with 40 ng/ml recombinant human (rh) FGF2 (R&D Systems), 40 ng/ml rhFGF8 (R&D Systems) ...
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bioRxiv - Biophysics 2019Quote: CCL5/RANTES was included in Human Chemokine Protein Sampler Pack from R&D systems..
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bioRxiv - Cell Biology 2020Quote: ... were incubated with 100μg/ml recombinant human Wnt10b protein (0.1mg/ml;R&D Systems) at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Human recombinant pro-legumain (asparaginyl endopeptidase) was purchased from R&D Systems (2199-CY), activated per manufacturer instruction and active site titrated using MP-L01 inhibitor as described by Poreba et al ...
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bioRxiv - Bioengineering 2019Quote: ... 0.004 % of recombinant human FGF basic (FGF-2, R&D Systems, Abingdon, United Kingdom) was added ...
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bioRxiv - Neuroscience 2019Quote: Recombinant human IDO (Accession #P14902) was obtained from R&D Systems (#6030-AO-010) and frozen at −80°C until use ...
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bioRxiv - Immunology 2021Quote: ... recombinant human IL-4 at 50 ng/ml (R&D Systems, Minneapolis, MN, USA) for 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant mouse interferon-γ and recombinant human interkeukin-8 (R&D Systems, Minneapolis, MN) was prepared in PBS containing 0.1% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... then labelled with and without anti-human LILRB2 (5 µg/mL; R&D Systems). The cells were washed with PBS (0.5% BSA ...
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bioRxiv - Bioengineering 2020Quote: ... and recombinant human IL-2 (50 U/mL, R&D Systems, Minneapolis, MN, USA). Single cell suspensions were activated with 1 μg/ml Concavalin A (Sigma ...
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bioRxiv - Biochemistry 2020Quote: The mouse and human anti-CHL1 antibodies (AF2147 and MAB2126; R&D systems, MN) were partially reduced and bound to HRP using a peroxidase labeling kit SH (Dojindo ...
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bioRxiv - Cancer Biology 2021Quote: ... Luciferase activity was confirmed using recombinant human TGFβ (R&D Systems, #240-B-010) treatment.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Recombinant human FGF23 and FGF2 were purchased from R&D Systems (Minneapolis, MN, USA). Synthetic human C-terminal FGF23 (residues 180-251 ...
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bioRxiv - Bioengineering 2021Quote: ... 100M PBMCs were thawed in 100 mL X-VIVO™ 10 media from Lonza (#04-380Q) with recombinant human IL-2 protein from R&D Systems (#202-IL). After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971 ...