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Citations for R&D Systems :
2551 - 2600 of 2664 citations for Recombinant Human CDK7 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Cells were stimulated for 18-24h in complete medium at 37˚C with addition of 50- 100U/ml human IFNγ (R&D Systems #285-IF-100).
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The concentration of IL-8 released in basolateral culture media were assayed by ELISA using the human ELISA development kits (DuoSet DY208, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Immunology 2024Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Neuroscience 2019Quote: EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328 ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins of interest were detected using the following primary antibodies: goat anti-TREM2 (R&D Systems, Inc., #AF1828), rabbit anti-PGRN (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: To assess the levels of hippocampal BDNF protein the BDNF Quantikine ELISA kit (R&D Systems, Wiesbaden, Germany) was used ...
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bioRxiv - Immunology 2020Quote: ... and CXCL12 protein concentration assayed with the Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... 50-100μg of protein lysate was treated for 6-8hrs with chABC (100 μU/mL; R&D systems) before being subjected to traditional western blotting ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were used: SOX9 (1:50, AF3045, R&D Systems, and AB5535, Millipore), ERG (1:50 ...
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bioRxiv - Immunology 2023Quote: Cytokine protein levels were determined either by ELISA (Enzyme-Linked ImmunoSorbent Assay) using kits from R&D Systems Inc ...
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bioRxiv - Bioengineering 2023Quote: ... 96-well plates were coated with anti-mouse protein C polyclonal sheep IgG (R&D Systems, Minneapolis, MN) and incubated at 4°C overnight ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Neuroscience 2019Quote: The secretion of cytokines in AD TREM2 neuronal cultures before and after stimulation with the Aß-S8C dimer was measured employing the Proteome Profiler Human Cytokine Array kit (R&D System, United States of America). The assay was performed following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... IP-10 and TGF-β levels were measured by enzyme-linked immunosorbent assay (ELISA) sandwich using human CXCL-10/IP-10 DuoSet ELISA (R&D Systems Inc, MN, USA) and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation ...
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bioRxiv - Cancer Biology 2019Quote: ... Human interleukin (IL)-8 and vascular endothelial growth factor (VEGF) were quantified in cell culture supernatants using DuoSet® ELISA (R&D Systems, Minneapolis, MN, USA), following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... IFN-γ concentrations in cell-free supernatants were determined by Human IFN-γ DuoSet enzyme-linked immunosorbent assay (ELISA; DY285; R&D systems, Minneapolis, MN, USA). EYFP−/EBFP+ dLP-CHO cells with no anti-hPD1 payload circuit were used as the control cells.
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bioRxiv - Cell Biology 2022Quote: The presence of angiogenic factors in BEC CM was evaluated trough a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D Systems Inc., Minneapolis, MN, USA). For this ...
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bioRxiv - Physiology 2020Quote: Serum samples were assayed for IL-6 concentration using commercially available human IL-6 enzyme linked immunosorbent assay kits (Quantikine®, R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Immunology 2024Quote: ... 2fTGH and HEK293T cells and ELISA was performed according to the manufacturer’s protocols for human IFN-β (R&D systems DY814-05PBL Assay Science 41410-2), human IFN-γ (Biolegend 43104 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor lysates were quantified and 40 μg protein loaded for analysis of GzB levels by ELISA (R&D Systems).
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bioRxiv - Bioengineering 2019Quote: ... and 0.005 N NaOH along with 10 ng/mL bone morphogenetic protein 4 (R&D Systems, Minneapolis, MN, USA). 4 × 105 cells were plated in each PDMS culture well with 3-mm pillar ...
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bioRxiv - Physiology 2020Quote: ... one multiplex panel was used to measure fatty acid binding protein 4 (FABP4) and resistin (R&D Systems, UK); non-esterified fatty acid (NEFA ...
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bioRxiv - Immunology 2022Quote: ... S proteins used in the study were SARS-CoV-2 S GCN4-IZ-2P (10561-CV, R&D Systems), SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... Direct binding of decorin protein core to the soluble Fc tagged VEGFR3 (sVEGFR3-Fc, R&D systems, 349-F4), was performed using solid-phase binding assay ...
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bioRxiv - Physiology 2024Quote: ... The levels of GDF15 protein in the serum were measured by an ELISA kit (Cat# MGD150, R&D Systems) following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems, 10549-cv) was determined by injecting two to four concentrations of SARS-CoV-2 RBD (50 nM highest concentration) ...
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bioRxiv - Physiology 2023Quote: ... The supernatants were diluted when necessary to be in the optimal linear optical range and analyzed using commercial kits for IFNγ (Human IFN-gamma DuoSet ELISA, R&D Systems, USA and Bio legend, UK), and IL-10 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: Catalytic activities of SpyCEP constructs were measured through detection of remaining intact CXCL8 or CXCL1 substrate following incubation with SpyCEP by ELISA (R&D Systems human CXCL8 and CXCL1 DuoSet ELISA) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations was determined by Thermo Fisher BCA assay kit and then 50μg of protein was loaded into the pre-coated VEGFA ELISA kit (R&D systems). Assay per manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... anti-FLT1 antibodies were incubated with mouse FLT1-FC chimeric protein with His-tag (471-F1-100, R&D Systems). After incubation the antibody-FLT1-FC complex was added to the VEGFA-coated plates ...
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bioRxiv - Pathology 2021Quote: ... Gdf8 and Smad2/3 proteins were quantified by ELISA methods (GDF8/Myostatin Quantikine ELISA kit, Cat # DGDF80, R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2023Quote: ... 200μg of total mouse protein samples were analyzed respectively with a Mouse XL Cytokine Array Kit (R&D Systems; ARY028) per manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: YM1 and CCL17 protein levels were measured in PCLS (4mm diameter) supernatant using commercially available ELISA kits (R&D Systems), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... KC protein from mouse brain homogenates was detected by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (R&D systems).
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bioRxiv - Systems Biology 2019Quote: ... CCL2 and CCL5 secreted protein levels in cell supernatants from 1×106 murine BMDM’s was performed by ELISA (R&D Systems) according to manufactureŕs instructions.
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bioRxiv - Molecular Biology 2019Quote: ... Urinary monocyte chemoattractant protein-1 (MCP-1) was measured using a commercially available ELISA kit (R&D Systems, Minneapolis MN, USA) as per the kit protocol ...
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IFNλ from intestinal biopsies were measured by Mouse IL28B/ IFN-lambda 3 DuoSet ELISA (DY1789B, R&D Systems). Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Death-related proteins were measured in cell lysates by a solid-phase antibody array with chemiluminescent detection (R&D Systems, 893900).
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bioRxiv - Cell Biology 2021Quote: ... which was constructed using the cDNA sequences of mouse IL-6/IL-6R alpha fusion protein (9038-SR, R&D systems). AAV8-Null vector was used as vector control.
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Microbiology 2019Quote: ... and lipocalin-2 (Lcn-2) protein was assayed in Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA). At the end of DSS treatment ...