Labshake search
Citations for R&D Systems :
2501 - 2550 of 2737 citations for Recombinant Human Carboxylesterase 3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Investigations into the effect of SmCI-1 and MutSmCI-1 on cytokine production were examined using a Human XL Cytokine Luminex Performance Panel Premixed Kit (bio-techne/R&D systems). Human blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Bioengineering 2022Quote: ... volume needed.25 Multiplexed Luminex assays were performed to measure cytokines in 48- or 72-hour undiluted conditioned medium throughout the co-culture period using the magnetic Human Luminex XL Cytokine 45- PLEX pre-mixed kit (R&D systems). Protocols provided by the manufacturer were adapted to allow the assay to be performed in a 384-well plate to avoid introducing batch variability ...
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bioRxiv - Cancer Biology 2022Quote: Human cytokine secretion was assessed in mouse plasma samples using the Human XL Cytokine Luminex Performance Panel Premixed Kit (#FCSTM18, R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were further processed following the Human Phospho-Receptor Tyrosine Kinase Kit (R&D Systems Europe, Ltd., Abingdon OX14 3NB, UK) manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Cell Biology 2022Quote: The cDNA encoding the extracellular N-terminal IgV domain of human CD47 (residues 19-141) was amplified from the plasmid hCD47 VersaClone cDNA (RDC1523, R&D systems) and was cloned in the expression vector pGEX-4T-1 (GE Healthcare) ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Neuroscience 2023Quote: ... Cytokine profiling was carried out following the manufactures instructions using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems; ARY022B). The kit contained membranes with dots of 105 different immobilised cytokine antibodies dotted with two technical replicates each (Supplementary Figure 5) ...
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bioRxiv - Pathology 2024Quote: ... Cytokines released from hiPSC-CMs into the supernatants were analyzed using Proteome Profiler Human XL Cytokine Array Kit (Cat. No. ARY022B R&D Systems). Chemiluminescence from the membrane was detected and scanned on an iBright Imaging System FL1000 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Microbiology 2023Quote: ... anti-hCD14 magnetic beads were used and isolated cells were differentiated to human monocyte-derived macrophages (hMDMs) by addition of 50 ng/ml rhM-CSF (R&D Systems) to XVivo medium (Lonza) ...
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bioRxiv - Microbiology 2023Quote: ... cell-free supernatant was collected and a semiquantitative immunosorbent assessment of cytokine expression was performed using a Proteome Profiler Human Cytokine Array Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 600,000 cells plus culture medium were replaced in a 24 well plate containing a 50-60% confluent monolayer of unstimulated ADSCs with or without addition of anti-human IL-1β (2ug/mL, R&D Systems) or a mouse IgG1 isotype control (2ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... cells from each well were harvested and resuspended in 100 µL of cell resuspension solution provided with human methylcellulose enriched media (R&D systems). Each cell suspension was added to 2.5 mL methylcellulose enriched media ...
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bioRxiv - Bioengineering 2023Quote: ... media was removed and frozen for secretomic screening assays for both VEGF-A (R&D Systems, Human VEGF DuoSet ELISA, Cat# DY293B) and VEGF-C (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... for 48 hours at which point the media was collected and analyzed for PAI-1 content using the Human Serpin E1/PAI-1 Quantikine ELISA Kit (DSE100, R&D Systems) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Release of cytochrome C from the mitochondria into the cytoplasm was assessed by a human cytochrome C Quantikine ELISA kit (Bio-techne R&D Systems) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Plasma levels of 14 chemokines were measured using the Luminex Discovery Assay - Human Premixed Multi-Analyte Kit (R&D Systems, LXSAHM-14) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
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bioRxiv - Cancer Biology 2022Quote: Freshly sorted m-LSCs from primary AMLs or prepared CD34+ HSPCs isolated from normal mobilized peripheral samples were plated in human methylcellulose (R&D systems) at about 100K/ml and 2K/ml ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Cancer Biology 2022Quote: Cytokine levels produced by homotypic and heterotypic cultures were analyzed using the Proteome Profiler™ Human Cytokine Array (ARY005B; R&D SYSTEMS) kit ...
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bioRxiv - Immunology 2023Quote: ... Levels of BAFF protein were determined in culture supernatant or bone marrow plasma using the human BAFF/BLyS/TNFSF13B DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
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bioRxiv - Physiology 2023Quote: Circulating TNFR1 and TNFR2 were measured in human or mouse samples using enzyme-linked immunosorbent assay (ELISA) kits (DY255, DY726, DY425 and DY426 respectively, all from R&D Systems) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 5 minutes on ice with a mixture of soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc (R&D systems) for the first scan or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc for the second scan ...
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bioRxiv - Cell Biology 2023Quote: We measured levels of secreted IL-10 in supernatants from infected or non-infected human primary macrophages cells using commercially available enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems) according to the manufacturers’ instructions.
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Cell Biology 2023Quote: The amount of VE-Cad and Rad51 protein in the conditioned media (CM) of PMVECs or human and rat plasma samples containing EDTA was quantitated by using ELISA kit (DCADV0, R&D systems for VE-Cad and custom made by MyBiosource for Rad51 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Neuroscience 2024Quote: The levels of secreted BDNF in cell culture supernates were measured using enzyme-linked immunosorbent assay (ELISA). ELISA kit for human BDNF (Cat. DBD00) was sourced from R&D Systems, and the experiments were conducted according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... we used both mouse (111 analytes) and human (105 analytes) proteome profiler XL kits (R&D Systems, Cat No. ARY028 and ARY022B). We screened the cell culture supernatant from Day 7 spheroid cultures with and without SIS ECM ...
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bioRxiv - Bioengineering 2023Quote: ... pulled secretome for each group was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (ARY007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... MSCs were characterized based on the criteria set out by the International Society for Cellular Therapy (ISCT)(ref) using the Human Mesenchymal Stem Cell Functional Identification Kit (R&D Systems) and Human Mesenchymal Stem Cell Verification kit (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and IgG in the culture supernatants from stimulated B cells were determined by ELISA kits for human MPO (R&D Systems, #DY3174), mouse MPO (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... and MCP-1 were determined in efflux collected on day 8 using a custom version of the Human XL Cytokine Performance Panel (R&D systems). SHBG levels were quantified using the Human XL Cytokine Discovery Panel (R&D systems) ...
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bioRxiv - Cell Biology 2024Quote: The enzyme-linked immunosorbent assay (ELISA) for human SPARC was performed using ready-to-go sandwitch assay kit with positive controls purchased from R&D Systems and following manifacturer’s guadlines ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... We incubated 1.5ml of media on Protease Arrays (R&D Systems Proteome Profiler Human Protease Array Kit, Cat # ARY021B; R&D Systems Inc. ...
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bioRxiv - Genetics 2024Quote: ... TGFB2 dosage in each eye (13 controls + 2 cases) was conducted with the Human TGF-beta 2 Quantikine ELISA kit (R&D Systems), following the provided instructions.
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bioRxiv - Cancer Biology 2024Quote: ... IL-6 concentrations in tissue lysates and CAF supernatants were measured using a commercially available kit (Human IL-6 DuoSet ELISA, R&D Systems). SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA ...
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bioRxiv - Cancer Biology 2024Quote: ... Duplicates of 50 μl of concentrate were subjected to IFN-β measurement by ELISA (Human IFN-beta DuoSET ELISA, R&D Systems), after 20X upconcentration (Amicon Ultracel-10 ...