Labshake search
Citations for R&D Systems :
2501 - 2550 of 2793 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: The levels of secreted BDNF in cell culture supernates were measured using enzyme-linked immunosorbent assay (ELISA). ELISA kit for human BDNF (Cat. DBD00) was sourced from R&D Systems, and the experiments were conducted according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... we used both mouse (111 analytes) and human (105 analytes) proteome profiler XL kits (R&D Systems, Cat No. ARY028 and ARY022B). We screened the cell culture supernatant from Day 7 spheroid cultures with and without SIS ECM ...
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bioRxiv - Bioengineering 2023Quote: ... pulled secretome for each group was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (ARY007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... MSCs were characterized based on the criteria set out by the International Society for Cellular Therapy (ISCT)(ref) using the Human Mesenchymal Stem Cell Functional Identification Kit (R&D Systems) and Human Mesenchymal Stem Cell Verification kit (R&D Systems).
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bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... APRIL and CD83 levels were measured in the serum/plasma of participants of the four study groups by using the Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit (R&D systems), APRIL Human ELISA Kit (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Immunology 2022Quote: Investigations into the effect of SmCI-1 and MutSmCI-1 on cytokine production were examined using a Human XL Cytokine Luminex Performance Panel Premixed Kit (bio-techne/R&D systems). Human blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Bioengineering 2022Quote: ... volume needed.25 Multiplexed Luminex assays were performed to measure cytokines in 48- or 72-hour undiluted conditioned medium throughout the co-culture period using the magnetic Human Luminex XL Cytokine 45- PLEX pre-mixed kit (R&D systems). Protocols provided by the manufacturer were adapted to allow the assay to be performed in a 384-well plate to avoid introducing batch variability ...
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bioRxiv - Cancer Biology 2022Quote: Human cytokine secretion was assessed in mouse plasma samples using the Human XL Cytokine Luminex Performance Panel Premixed Kit (#FCSTM18, R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Cell Biology 2022Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
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bioRxiv - Cancer Biology 2022Quote: ... The samples were further processed following the Human Phospho-Receptor Tyrosine Kinase Kit (R&D Systems Europe, Ltd., Abingdon OX14 3NB, UK) manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Plasma levels of 14 chemokines were measured using the Luminex Discovery Assay - Human Premixed Multi-Analyte Kit (R&D Systems, LXSAHM-14) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Freshly sorted m-LSCs from primary AMLs or prepared CD34+ HSPCs isolated from normal mobilized peripheral samples were plated in human methylcellulose (R&D systems) at about 100K/ml and 2K/ml ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Cancer Biology 2022Quote: Cytokine levels produced by homotypic and heterotypic cultures were analyzed using the Proteome Profiler™ Human Cytokine Array (ARY005B; R&D SYSTEMS) kit ...
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bioRxiv - Immunology 2023Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
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bioRxiv - Physiology 2023Quote: Circulating TNFR1 and TNFR2 were measured in human or mouse samples using enzyme-linked immunosorbent assay (ELISA) kits (DY255, DY726, DY425 and DY426 respectively, all from R&D Systems) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 5 minutes on ice with a mixture of soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc (R&D systems) for the first scan or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc for the second scan ...
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bioRxiv - Cell Biology 2023Quote: We measured levels of secreted IL-10 in supernatants from infected or non-infected human primary macrophages cells using commercially available enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems) according to the manufacturers’ instructions.
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Immunology 2024Quote: ... and IgG in the culture supernatants from stimulated B cells were determined by ELISA kits for human MPO (R&D Systems, #DY3174), mouse MPO (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... and MCP-1 were determined in efflux collected on day 8 using a custom version of the Human XL Cytokine Performance Panel (R&D systems). SHBG levels were quantified using the Human XL Cytokine Discovery Panel (R&D systems) ...
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bioRxiv - Cell Biology 2024Quote: The enzyme-linked immunosorbent assay (ELISA) for human SPARC was performed using ready-to-go sandwitch assay kit with positive controls purchased from R&D Systems and following manifacturer’s guadlines ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... We incubated 1.5ml of media on Protease Arrays (R&D Systems Proteome Profiler Human Protease Array Kit, Cat # ARY021B; R&D Systems Inc. ...
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bioRxiv - Genetics 2024Quote: ... TGFB2 dosage in each eye (13 controls + 2 cases) was conducted with the Human TGF-beta 2 Quantikine ELISA kit (R&D Systems), following the provided instructions.
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bioRxiv - Neuroscience 2024Quote: The concentration of ELANE in the patient plasma samples was measured using a Human Neutrophil Elastase/ELA2 DuoSet ELISA kit (DY9167, R&D Systems). ELANE expression in mouse corpus callosum areas and plasma samples was measured using commercial ELISA kits (MELA20 ...
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bioRxiv - Microbiology 2024Quote: ... The UCP conjugates for neural cell adhesion molecule (NCAM/CD56) and serpin-A3 were prepared with goat anti-human NCAM Ab (AF2408, R&D systems) and goat antihuman serpin-A3 (AF1295 ...
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bioRxiv - Immunology 2024Quote: ... The supernatants were preserved at −20°C until they were assessed for IL-2 concentration using Human IL-2 DuoSet ELISA kit (DY202, R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Cell culture supernatants were also collected from 96-well plate infections at 9 hpi and secreted IL-1β measured by DuoSet© Human IL-1β/IL-1F2 ELISA as per manufacturer’s instructions (R&D Systems). A core genome SNP ML phylogeny was constructed on the Australian ST10 lineage to demonstrate the genetic relationship of representative ST10 isolates that underwent in vitro phenotypic assays.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with PBS and incubated with an anti-human CCR6 mouse monoclonal antibody (1:50, R&D Systems, U.S.A.) and an anti-CD4 rabbit monoclonal antibody (1:30 ...
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bioRxiv - Immunology 2024Quote: ... in either 10% chicken sera in 5% BSA/PBS (immunofluorescence) and probed with goat anti-human MPO (detects mouse MPO, AF3667 R&D Systems) rat anti-mouse CD45 (all leukocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... IL-6 concentrations in tissue lysates and CAF supernatants were measured using a commercially available kit (Human IL-6 DuoSet ELISA, R&D Systems). SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA ...
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bioRxiv - Cancer Biology 2024Quote: ... Duplicates of 50 μl of concentrate were subjected to IFN-β measurement by ELISA (Human IFN-beta DuoSET ELISA, R&D Systems), after 20X upconcentration (Amicon Ultracel-10 ...
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bioRxiv - Genomics 2024Quote: ... diluted 3 times with Immunocult- XF medium without IL-2 and used for cytokine quantification with Human IL-2 or IFNγ DuoSet (R&D Systems) enzyme-linked immunosorbent assay and read out using an Infinite F200 Pro (Tecan ...
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bioRxiv - Bioengineering 2024Quote: Cytokine profiling of the EV groups was performed using the dot blot-based Proteome Profiler Human XL Cytokine Antibody Array kit (R&D Systems) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cells were grown for 72 hours after which culture media was collected and immediately assayed for IL-6 levels (Human IL-6 Quantikine Elisa, R&D Systems). Optical density at 540nm was determined using a SPECTROstar Omega (BMG Labtech) ...
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bioRxiv - Cancer Biology 2024Quote: ... Kuramochi) was evaluated 24 h after changing the medium using the Human IL-6 DuoSet ELISA Kit (DY206-05, R&D Systems) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: Supernatants of three biological replicates were harvested 24 h after medium change and Proteome Profiler Human XL Cytokine array (R&D Systems) analysis was performed following the manufacturer’s protocol and signals were detected ...
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bioRxiv - Cancer Biology 2024Quote: ... and an ELISA was performed with three biological replicates on 10-fold diluted medium using human IFNɣ DuoSet ELISA kits (R&D Systems). The absorbance was recorded at 450 nm using a microplate absorbance reader (Tecan ...
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bioRxiv - Molecular Biology 2020Quote: L-FABP levels in human and mouse sera were measured using enzyme-linked immunosorbent assay kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...