Labshake search
Citations for R&D Systems :
2451 - 2500 of 2561 citations for Recombinant Mouse PGF since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Western blotting was carried out using mouse monoclonal antibody clone M2 directed against the FLAG-tag on Npn2 (SigmaAldrich #F3165) or rabbit anti-NrCAM antibody (R&D Systems AF8538) and Avansta enhanced chemiluminescence ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was immediately used to determine the levels of IL1β and IL6 using Quantikine Mouse ELISA kits and following the manufacturer’s protocols (R&D Systems, Minneapolis, Mn).
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Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)bioRxiv - Biochemistry 2021Quote: ... We collected the cell culture supernatant and measured the TNF-α concentration using Mouse TNF-α Quantikine ELISA Kit MTA00B (R&D SYSTEMS).
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bioRxiv - Immunology 2020Quote: ... except for the STORM experiments where they were used at a concentration of 20 μg/ml: α-MerTK (mouse extracellular monoclonal, 125618, R&D Systems), α-MerTK (goat extracellular polyclonal ...
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bioRxiv - Immunology 2021Quote: ... Each pooled sample was assayed for 40 different cytokines using the Proteome Profiler Mouse Cytokine Array Panel A kit according to manufacturer’s instructions (R&D Systems, Minneapolis, MN). Membranes were imaged using a LI-COR Odyssey Fc chemiluminescence imager (Lincoln ...
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bioRxiv - Immunology 2021Quote: ... Primary antibody (Anti-mouse RAE-1 pan-specific antibody, recognizing RAE-1α, β, δ, γ and ε (R&D Systems, Catalogue Number AF1136) or an appropriate isotype-matched control (polyclonal goat isotype IgG ...
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bioRxiv - Cell Biology 2021Quote: ELISAs were performed on sera collected from mice at terminal bleed with the following kits per manufacturers’ instructions: Mouse IL-1β/IL-1F2 (R&D Systems, MLB00C), mouse TNF-alpha (R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647, clone H398, lot 0410; or APC-labelled anti-mouse gp130, R&D Systems, cat #FAB4681A ...
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bioRxiv - Cancer Biology 2022Quote: ... IFNγ released into the supernatant by CAR-T cells was then measured using the DuoSet ELISA kit for mouse IFNγ (R&D systems, DY485) and Nunc MaxiSorp flat bottom plates (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Immunology 2024Quote: IFN-γ protein levels were measured from cryopreserved neuron-CD8+ T cell co-culture supernatant using the Mouse IFN-γ DuoSet ELISA kit from R&D Systems (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Protein lysates were subjected to enzyme-linked immunosorbent assay (ELISA) using mouse DuoSet® ELISA commercial kits (R&D Systems, Minneapolis, MN, USA), following manufacturer instructions.
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bioRxiv - Immunology 2023Quote: Levels of human IL-18 and mouse IL-1β were measured in the cell supernatants using ELISA kits from R&D Systems (DY318) and (DY401-05) ...
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bioRxiv - Neuroscience 2022Quote: IL-1β in microglia culture supernatant was measured with Mouse IL-1β /IL-1F2 DuoSet ELISA Kit (R&D Systems DY401, Minneapolis MN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Plasma C-reactive protein levels were assessed by a Mouse C-Reactive Protein/CRP Quantikine ELISA Kit (R&D Systems, Inc., MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: All mouse serum samples were analyzed by a custom R&D systems Murine Premixed Multi- Analyte Kit (R&D Systems, Inc., Minneapolis, MN). The panel was run on a Luminex 200 Instrument (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: IFN-γ and granzyme B concentrations in the supernatants of CD8+ T cells were measured using a mouse ELISA kit (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cytokine levels of TNFα was quantified in serum obtained from mice infected with and without Salmonella for 24 h by the mouse/rat TNF-A Valukine ELISA kit (R&D Systems, #VAL609) following the product protocol.
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bioRxiv - Biochemistry 2023Quote: ... Serum Cystatin C levels were measured using the Mouse/Rat Cystatin C Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, MSCTC0). Blood Nitrogen Urea serum levels were measured using the QuantiChrom Urea Assay Kit (BioAssay Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... male mice bearing LNAI subcutaneous tumors were assayed for systemic cytokines using the Proteome Profiler Mouse XL Cytokine array (R&D Systems, ARY028). Membranes were exposed on film using the provided chemiluminescent reagent ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant of BALF was store at -80°C for cytokine analysis by mouse TNF-α and IL-1β DuoSet ELISA kits (R&D Systems). Lungs were perfused with a 1:1 mixture of OCT and 4% PFA and preserved in OCT cryomolds (Tissue-Tek ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was probed with an anti-EFA6R rabbit polyclonal antibody (1:500) (8) or an anti-β-actin mouse monoclonal antibody (1:10,000) (R&D Systems, UK) diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of epidermal growth factor (EGF) protein levels in the serum was performed with the Mouse EGF Quantikine ELISA Kit (MEG00, R&D Systems Inc.) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: Blood serum was analyzed using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay (MCYTMAG-70K-PX32, MilliporeSigma) and Proteome Profiler Mouse XL Cytokine Array (ARY028, R&D Systems Inc,) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Parallel experiments included the addition of a neutralizing anti-IL-15 MoAb (10 mg/ml) (mab247, mouse IgG1, R&D systems, Abingdon, UK). Alternatively ...
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bioRxiv - Physiology 2024Quote: ... USA) and blood FGF21 levels were assayed by a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Microbiology 2020Quote: Mouse CXCL1/KC and interleukin-6 concentrations in lung homogenates from infected mice were determined using DuoSet® ELISA kits (R&D Systems, UK) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: A separate experiment employed the culture and counting methods described above for irisin treatment in combination with a neutralizing antibody for integrin αVβ5 (Anti-Integrin aVb5, MilliporeSigma, Burlington, MA, USA) and an IgG control (Mouse IgG1 Isotype Control, R&D Systems, Minneapolis, MN, USA), each supplied continuously in the media during the 7 day culture at 0.9 µg/ml.
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bioRxiv - Microbiology 2022Quote: ... Blots were incubated for 1 hour with HRP-conjugated secondary antibodies diluted in 5% skim milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Molecular Biology 2020Quote: Adiponectin levels in the gonadal white adipose tissue was determined using Quantikine mouse adiponectin/Acrp 30 ELISA kit from R&D systems (MN, USA). Liver homogenates were measured by a solid-phase ELISA technique according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: FGF-21 levels in the serum and liver were determined using Quantikine mouse/rat FGF-21 ELISA kit from R&D systems (MN, USA). Serum and liver homogenates were measured by a solid-phase ELISA technique according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: IGF-1 levels in the serum and tissues (liver, gastrocnemius, and brain cortex) were determined using Quantikine mouse IGF-1 immunoassay from R&D systems (MN, USA). Serum samples were diluted in calibrator diluent provided in the kit at 500-fold dilution and assayed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: CXCL12 concentration in supernatants of CXCL12 Ch/DS NC was quantified using Mouse CXCL12 DuoSet ELISA as per supplier instructions (R&D systems, Minneapolis, USA). Encapsulation efficiency and drug loading were calculated as follow:
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bioRxiv - Immunology 2019Quote: ... sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained in 0.5% fatty acid-free albumin in DPBS using 1:20 mouse IgG3 SSEA4-488 clone MC-813-70 (R&D Systems, FAB1435F, lot YKM0409121) and 1:20 mouse IgM TRA-1-60-488 clone TRA-1-60 (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... secreted IL-2 samples were diluted 1:10 and subsequently analyzed using Mouse IL-2 Quantikine ELISA Kit (R&D Systems Inc, Minneapolis, MN), as per manufacturer’s specifications.
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bioRxiv - Bioengineering 2021Quote: ... Kidney tissue sections were incubated with 1μg/mL of mouse-α-Tubulin primary antibody and Goat-α-CD31 primary antibody (R&D Systems, AF3628) in 0.1% Triton-X/PBS at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... 2×105 isolated TILs have been cultured in vitro in 96well plate in presence of 20ng/ml mouse IL-2 (R&D System 402-ML), 20ng/ml mouse IL-7 (R&D System 407-ML ...
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bioRxiv - Cell Biology 2021Quote: ... and diluted to equal concentrations before performing an enzyme-linked immunoabsorbent assay (ELISA) using a Mouse/Rat FGF21 Quantikine ELISA kit (R&D Systems, Minneapolis, MN)(57).
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bioRxiv - Microbiology 2021Quote: Virus-inactivated serum samples or lung supernatants from SARS-CoV-2 VoC infected mice were added to a custom 8-plex Mouse Magnetic Luminex Assay (R&D Systems LXSAMSM-08) including IL-6 ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of IP10 in the media was then determined by ELISA using the Mouse CXCL10/IP-10/CRG2 DuoSet ELISA from R&D system (cat. DY466) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the blood was centrifuged with 2000g for 20min and the supernatant (serum) was collected for TPO measurement using Quantikine Mouse Thrombopoietin ELISA Kit (R&D Systems, Minneapolis, USA) to measure serum TPO levels ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Immunology 2022Quote: Naive CD4+CD25- T cells from the LNs and spleen of WT or NAC1-/- mice were incubated with the indicated reagents including TGF-β in the CellXVivo™ Mouse Treg Cell Differentiation Kit (#CDK007, R&D Systems) for 5 days.
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein was applied to each ELISA membrane and developed according to the manufacturer’s instructions (Proteome Profiler Mouse XL Cytokine Kit, ARY028, R&D Systems, Minneapolis, MN, USA). Cytokine array signals were detected by X-ray film ...