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Citations for R&D Systems :
201 - 250 of 1144 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... fibroblasts were mixed with Cultrex rat tail collagen 1 (cat. # 3447-020-01, R&D Systems, 5mg/ml) for a final 1mg/ml collagen concentration ...
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bioRxiv - Physiology 2020Quote: ... Lysates were harvested and intracellular cAMP levels were assayed using the cAMP Parameter Assay Kit (R&D Systems, Minneapolis, MN).
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bioRxiv - Cell Biology 2021Quote: BDNF concentration in the plasma and brain lysates were quantified using a DuoSet BDNF ELISA Kit (R&D Systems, DY248) according to manufacturer instructions ...
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bioRxiv - Physiology 2020Quote: Plamatic IgE level was evaluated by SDS PAGE using rat anti-mouse IgE antibodies (R&D systems Cat# MAB9935).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ELISA was performed using the Rat IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The specificity of staining was confirmed by staining with rat IgG (ref:6-001-F, R&D Systems, Biotechne) or rabbit IgG (ab27478 ...
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bioRxiv - Molecular Biology 2020Quote: ... Goat anti-SOX17 (cat# AF1924) and rat anti-SSEA3 (clone MC-38, cat# MAB1434) were from R&D Systems. Mouse anti-CD184 (CXCR4) ...
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bioRxiv - Physiology 2020Quote: ELISA for serum leptin was conducted using a mouse/rat Leptin Quantikine ELISA Assay kit (R&D Systems, MOB00) at a concentration of 1:2 in LD mice ...
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bioRxiv - Physiology 2019Quote: ... GDF15 levels were measured using Mouse/Rat GDF15 Quantikine ELISA Kit (Cat#: MGD-150, R&D Systems, Minneapolis, MN) according to the manufacturers’ instructions ...
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bioRxiv - Physiology 2022Quote: ... mouse IgG2a kappa (eBM2a) (eBioscience™, 14-4724-82) and the rat IgG (R&D Systems, 6- 001-F).
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bioRxiv - Cell Biology 2023Quote: ... starved for 2 hours and treated with 10 ng/ml rat recombinant EGF (R&D Systems, Minneapolis, MN, USA) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used in the study: anti-rat monoclonal S100A8 (Cat: MAB3059; R&D Systems; Minneapolis, MN) and anti-goat Iba1 (Cat ...
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bioRxiv - Immunology 2023Quote: ... a 50:50 mixture of Matrigel Matrix GF and 4 mg/ml Cultrex Rat Collagen I (R&D Systems). For time-lapse live imaging experiments ...
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bioRxiv - Cell Biology 2023Quote: Microfluidic devices were prepared as described previously [32] using 2.5 mg/mL Collagen I (rat tail, R&D Systems) as extracellular matrix ...
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bioRxiv - Physiology 2024Quote: ... Circulating FGF21 was quantified using a mouse/rat FGF21 quantikine ELISA kit (MF2100; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... Blots of spleen lysates were prepared with a Proteome Profiler Mouse Cytokine Array Kit (Panel A, R&D Systems, Minneapolis, MN) using an established protocol.[23] After blot development ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Developmental Biology 2022Quote: IGF2 measurements in E13.5 whole-embryo lysates were performed with the Mouse IGF-II DuoSet ELISA kit (R&D Systems – DY792), using an assay adapted for the MesoScale Discovery electrochemiluminescence immunoassay platform (MSD) ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was loaded with 30 µL (50 µg protein) lysate with 70 µL of Buffer A buffer containing 20μM Suc-LLVY-AMC (R&D Systems). Assay parameters ...
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bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Cell Biology 2022Quote: Plasma collected using EDTA was used for FGF21 determination by Mouse/Rat FGF-21 Quantikine ELISA Kit (R&D System) per manufacture’s instruction.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Bioengineering 2022Quote: ... The tissue section slides were fixed and stained with a rat anti-intelectin monoclonal antibody (R&D Systems, Clone 746420) with fluorescent detection and DAPI (ProLong antifade reagent with DAPI) ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant factors (murine FGF7, Peprotech, 450-60; human BMP3, Peprotech, 120-24B; rat CTGF, R&D Systems, 92-37C-T050) were resuspended in 0.1% BSA/PBS and used at final concentration 100 ng/mL.
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bioRxiv - Biochemistry 2023Quote: ... were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml, R&D systems 3440-100-01) at 37°C in 5% CO2 in high glucose-containing DMEM (Corning 10-013-CM ...
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bioRxiv - Immunology 2023Quote: ... Serum FGF21 concentration was determined by ELISA using the mouse/rat FGF21 commercial kit from R&D Systems (Minneapolis, MN). Serum samples were run at a 1:10 dilution.
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bioRxiv - Cancer Biology 2023Quote: ... and primary OC-CAFs were incorporated into a 3D matrix made of rat tail type 1 collagen (R&D Systems) as previously described 95 ...
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bioRxiv - Neuroscience 2021Quote: Protein was harvested from WT and p.Glu355Lys iPSC and NCC lysates (d19 of differentiation) using the manufacturer’s Receptor Tyrosine Kinase (RTK) array protocol in Lysis buffer 17 (R&D Systems, # ARY001B). RTK arrays were blocked for 1 hour at room temperature in Array Buffer 1 (manufacturer’s protocol) ...
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bioRxiv - Microbiology 2021Quote: IL-1β and IL-6 were detected in tissue lysate using the Mouse IL-1β and IL-6 DuoSet ELISA kits (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
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bioRxiv - Cancer Biology 2023Quote: ... caspase-3 and PARP cleavage were determined by Western Blot analysis while levels of PAR in the tumor lysates were determined by ELISA using the HT PARP in vivo PD Assay II Kit [4520-096-K] from R&D Systems. The remaining mice were measured twice weekly in three dimensions using Vernier calipers and the volume estimated using the formula (width x length x depth x 0.524mm3) ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of pre-cleared lysates was incubated with 50 µL Dynabeads Protein G (30 mg/ml) and 5 µg Regnase-1 (R&D systems) or Regnase-3 (Helmholtz Munich Monoclonal Antibody Core Facility ...
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50 ...
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bioRxiv - Cell Biology 2021Quote: We performed a Phage-Fab (antigen-binding fragment) selection using a naïve Fab library (libF46) on an immobilized recombinant rat Unc5B-ECD Fc fusion protein (R&D systems). Phage particles fused with Fabs were incubated with an unrelated protein (e.g ...
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bioRxiv - Neuroscience 2020Quote: ... coated with 1 µg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (clone 237920, R&D Systems, RRID: AB_2208679) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... MSD standard-bind microtitre plates were first coated with 30µl capture antibody (Rat Anti-Mouse IGF-II, R&D Systems – 840962) diluted to 7.2 µg/ml in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Serum IGF-1 levels were measured by Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA). Serum parameters were measured and calculated with a VITROS analyzer (Ortho Clinical Diagnostics ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used for western blot were used as follows: rat anti-IL-33-unconjugated (1:1000; R&D Systems MAB3626), rat anti-MCP1/Mcpt1 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated overnight with unconjugated goat anti-mouse/rat CD47 N-terminal IgV-like extracellular domain antibody (R&D Systems), biotin-conjugated anti-mouse CD55 REAfinity antibody (clone REA300 ...
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bioRxiv - Genomics 2023Quote: ... anti-FcγRII/III (rat anti-mouse, clone 190909, cat. MAB1460), and anti-FcγRI (rat anti-mouse, clone 29035, cat. MAB2074) were purchased from R&D systems. 40μg of anti-FcγRII/III and 20μg of anti-FcγRI was used in FcγR-blocking conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Activin A levels were measured using an ELISA kit (Human/Mouse/Rat Activin A Quantikine ELISA Kit, R&D Systems, DAC00B).
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bioRxiv - Cell Biology 2023Quote: ... Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin, 1:400 for IF) was from R&D System (#AF3235). Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain ...
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bioRxiv - Microbiology 2024Quote: ... Cytokine and chemokines levels were measured using a commercial multiplex immunoassay (Proteome Profiler rat XL Cytokine Array Kit; R&D system) which allows the detection of 79 rat cytokines and chemokines according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ELISA kits were used to determine the levels of GDF15 (mouse/rat GDF15 Quantikine ELISA Kit, R&D Systems, catalog # MGD150), TIMP-1 (mouse TIMP-1 Quantikine ELISA Kit ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...