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Citations for R&D Systems :
201 - 250 of 262 citations for 9 Z 12 E OCTADECADIENOIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... IL-12 and recombinant human TGF-β1 were purchased from R&D Systems (Abingdon, UK).
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Barrier integrity was further assessed using the Human E-cadherin Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN). Absorbance was measured at 450 nm using a BioTek Synergy H2 plate reader and concentrations of E-cadherin were determined against a standard curve according to manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and the supernatant was used for the quantification of IL-1β e IL-6 cytokines using commercial kits (R&D System®).
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bioRxiv - Genetics 2020Quote: ... cells were grown until colonies were visible and subsequently passaged at low densities on Biolaminin 521 LN® (Biolamina) coated 24 well plates supplemented with 1.5 µg/mL E-Cadherin (R&D systems) to obtain single cell colonies ...
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bioRxiv - Microbiology 2023Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1 (R&D systems, Cat# E-522B), 2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: Cells in 60 wells were incubated with primary goat polyclonal anti-mouse E-cadherin (R&D Systems, Cat. No AF748, 1:200) in cold 5% FBS/PBS for 20 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... staining for anti-murine Fatty acid binding protein 4 (Fabp4) (R&D Systems, AF1443) was used ...
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bioRxiv - Immunology 2022Quote: ... and/or cells were activated with recombinant human IL-12 and IL-18 (R&D Systems), or IL-2 and IL-33 ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were primed for induction with 12 ng/mL FGF2 (R&D Systems, Cat# 233-FB) and 10 ng/mL activin A (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St. Louis, MO, USA; LBP from R&D Systems, Abingdon, UK), isotype control (rat IgG2b ...
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bioRxiv - Molecular Biology 2019Quote: ... Additional reagents used: 2N Sulfuric Acid stop solution (catalog # DY994, R&D Systems, Minneapolis, MN). Triphenylphosphine (TPP ...
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bioRxiv - Cell Biology 2020Quote: ... citric acid antigen retrieval was performed prior to immunohistochemistry for GATA4 (R&D Systems, AF2606). Staining was visualized using R.T.U ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with either DMSO or 5 µM Idasanutlin (R&D Systems, #12-35-22-07) for an additional 24 h before cells were harvested for protein and RNA extraction.
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bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
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bioRxiv - Pathology 2021Quote: ... 1 million cells per well were plated in 12-well Cultrex coated (R&D Systems, 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2mM ascorbic acid and 1 ng/mL recombinant human FGF (R&D system, 233-GMP-025). The adherent cells were split by trypsinization at 80% confluency and frozen at passage 2 (50% αMEM ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in N2B27 medium supplemented with 12 ng/ml bFGF (R&D Systems, cat. 233-FB-025/CF), 20 ng/ml Activin A (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL of IL-2 and 10 ng/mL of IL-12 (R&D systems, 419-ML-010)) ...
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bioRxiv - Immunology 2023Quote: ... Purified cells from five subsets were cultured in IL-12 (20 ng/ml, 219-IL-005; R&D Systems), IL-18 (20 ng/ml 9124-IL-010 ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were serum-starved for 12 h and then treated with 100 ng/ml BMP2 (R&D Systems) for 3 h ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Bioengineering 2021Quote: ... 12 million cells were purified for CD90+ cells using a PE-conjugated antibody for anti-CD90 (R&D Systems, FAB2067P), the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... enzyme linked immunosorbent assay (ELISA) kit for IL-12/IL-23 (p40) was obtained from R&D Systems (Minnesota, USA); ELISA kit for IL-6 ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were first differentiated into macrophages with 50 nM Phorbol-12-Myristate-13-Acetate (PMA) (R&D systems) for 72 hrs ...
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bioRxiv - Neuroscience 2021Quote: ... coated plates in N4 media (N3 with 0.05uM Retinoic acid, 2ng/ml BDNF (R&D Systems #248-BDB) and 2 ng/ul GDNF (R&D Systems #212-GD)) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blotted using the primary antibodies: Ebi3 (2.5 μg/ml; eBioscience) or IL-12/IL-35 p35 (1 μg/ml; R&D Systems), phospho-mTOR (Ser2448 ...
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bioRxiv - Systems Biology 2019Quote: ... Sera were subjected to depletion of abundant serum proteins using Proteome Purify 12 Human Serum Protein Immunodepletion Resin (R&D Systems). Denaturing was followed by alkylation with N-ethylmaleimide and acetone precipitation ...
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bioRxiv - Bioengineering 2021Quote: ... 12 million cells were then purified for CD90+ cells using a PE-conjugated antibody for anti-CD90 (R&D Systems, FAB2067P), the EasySep™ Human PE Positive Selection Kit II and EasySep™ Magnet.
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 12-well plates and treated for 8 hours in biological quadruplicates with IFNα (0.5 ng/ml, R&D Systems), IFNβ (0.5 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml soluble anti-CD28 and cultured in the absence (Th0) or presence of 2.5 ng/ml IL-12 (R&D Systems) (Th1 ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Additional reagents that were used are 2N Sulfuric Acid Stop Solution (Catalog no. DY994, R&D Systems, Minneapolis, MN), Triphenylphosphine (TPP ...
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bioRxiv - Physiology 2020Quote: ... one multiplex panel was used to measure fatty acid binding protein 4 (FABP4) and resistin (R&D Systems, UK); non-esterified fatty acid (NEFA ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were converted to an epiblast-like (EpiLC) state by 48 hour culture in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Microbiology 2021Quote: ... IL-1β (cat No. DY401) and IL-12 p70 (cat No. DY419) by using the mouse DuoSet ELISA system (R&D Systems). In addition ...
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bioRxiv - Microbiology 2022Quote: Cover glass (FisherBrand, 12-546-P) was sterilized in 70% EtOH and coated with BME Cultrex (R&D Systems, 3432-010-01) prior to seeding ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
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bioRxiv - Microbiology 2023Quote: ... The culture supernatants were analyzed for cytokines using the Human TNF-α and Human IL-12 DuoSet ELISA kits (R&D Systems) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested after treatment and stained for PD-L1 (eBioscience, 12-5983-42) and MHC class I (R&D Systems, FAB7098G). Induction of the respective proteins was analyzed by flow cytometry.
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bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Cell Biology 2020Quote: ... Retinal crossections (12 µm) were processed for immunostaining using the following antibodies: rat monoclonal to CD45 antibody (clone 30-F11, R&D Systems, 1:50). Sections were pre-incubated with 5% goat serum (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The floating cells were collected by centrifugation and the cells were resuspended in microglia media (DMEM/F12 + 1X Glutamax + 1X Sodium pyruvate + 0.1% of fatty acid-free bovine serum albumin and 1X penicillin/streptomycin + 1X ITS supplement (R&D Systems AR013) + 25 ng/mL M-CSF (GoldBio 1320-09-10) ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...