Labshake search
Citations for R&D Systems :
201 - 250 of 296 citations for 6 decenoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... IL-6 and TNF-α were measured by enzyme-linked immunosorbent assays (ELISAs) using kits purchased from R&D Systems (DY400-05 ...
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bioRxiv - Immunology 2020Quote: ... IL-10 and IL-6 were measured by sandwich ELISA using the Duo Set ELISA kit (R&D Systems, UK) (23 ...
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bioRxiv - Cell Biology 2020Quote: ... retinae from C57bl6 and Cx3cr1GFP/GFP animals (n = 6) were incubated as above with fractalkine (200ng/ml, R&D Systems) or PBS for 2 hours at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems; Minneapolis, MN, USA) and 25 ng/mL recombinant human leukemia inhibitory factor (LIF ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-6 in cell culture supernatant were determined using the mouse DuoSet kit (R&D Systems, Minneapolis, MN). All assays were performed according the manufacturer’s supplied protocol ...
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bioRxiv - Neuroscience 2022Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... IL-6 and IL-8 levels in conditioned media were quantified using Quantikine immunoassays (HS600C and D8000C, R&D systems). GFAP was measured in CM using Human GFAP DuoSet ELISA (R&Dsystems ...
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bioRxiv - Physiology 2023Quote: ... and IL-6 levels were quantified in BALF and lung tissues using commercially available DuoSet ELISA kits (R&D Systems) according to manufacturer’s instructions and published protocols 22 ...
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bioRxiv - Cell Biology 2022Quote: ... The floating cells were collected by centrifugation and the cells were resuspended in microglia media (DMEM/F12 + 1X Glutamax + 1X Sodium pyruvate + 0.1% of fatty acid-free bovine serum albumin and 1X penicillin/streptomycin + 1X ITS supplement (R&D Systems AR013) + 25 ng/mL M-CSF (GoldBio 1320-09-10) ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Cell Biology 2021Quote: The cell culture medium was pre-heated to 60°C for 5 mins prior to the addition of fatty acids (400μM oleic acid or 400μM palmitic acid or ethanol control), without (DMSO control) or with DGAT inhibitors as specified (A922500, PF04620110 or PF 06424439 (R&D system)) ...
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bioRxiv - Immunology 2023Quote: ... Total TGF-β levels were determined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems).
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bioRxiv - Biophysics 2020Quote: ... Purified mouse CD45RABC extracellular domain with a C-terminal 6-His tag (accession #: NP_001104786, catalog number: 114-CD-050) was purchased from R&D systems. Lectins Sambucus Nigra Lectin (SNA ...
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bioRxiv - Cell Biology 2020Quote: mOSE cells (1×10^6 cells) were plated 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). RNA was collected 7 days post-TGFB1 treatment (RNAeasy Kit ...
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bioRxiv - Immunology 2021Quote: ... Nunc® Maxisorp™ 384-well plates were coated with 6 µg/ml of recombinant mouse pIgR protein (R&D Systems) diluted in coating solution concentrate (Seracare ...
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bioRxiv - Genetics 2019Quote: ... counted and seeded at 5.5 × 106 cells per well in 6-well suspension plates in 5.5 ml hESC media supplemented with 10 ng /ml activin A (R&D Systems) and 10 ng/ ml heregulinB (Peprotech) ...
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bioRxiv - Cell Biology 2021Quote: ... which was constructed using the cDNA sequences of mouse IL-6/IL-6R alpha fusion protein (9038-SR, R&D systems). AAV8-Null vector was used as vector control.
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bioRxiv - Neuroscience 2022Quote: ... Interleukin-6 was measured in duplicate using a commercially available high-sensitive enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis) with a minimum detection threshold of 0.2 pg/ml ...
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bioRxiv - Microbiology 2019Quote: The human IFN-β ELISA Kit (cat. #41410-1) and IL-6 ELISA kit (cat. #D6050) purchased from R&D systems were used to measure the Chlamydia-induced IFN-β and IL-6 respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... human cytokines (1 ng/ml stem cell factor, 100 ng/ml thrombopoietin, 13.5 ng/ml interleukin 9, 10 ng/ml interleukin 6; all cytokines from R&D Systems), and 1XPenicillin-Streptomycin (Gibco ...
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bioRxiv - Immunology 2023Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Nrp1-Fc fusion protein containing the extracellular domain of rat Nrp1 (amino acid 22-854 minus 811-828; R&D Systems, 566-NNS) or the control human Fc dimer (R&D Systems ...
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bioRxiv - Physiology 2020Quote: ... non-esterified fatty acid (NEFA) serum levels were measured by colorimetric assay (Randox, UK) and dipeptidyl peptidase 4 (DPP4; R&D Systems, UK) levels were measured by ELISA.
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bioRxiv - Genomics 2024Quote: ... 5 mM beta-glycerophosphate and 1% insulin-transferrin-selenous acid (osteogenic media) and stimulated them the next day with recombinant human BMP2 (300 ng/ml) (R&D Systems, MN) in serum-free osteogenic media ...
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bioRxiv - Cell Biology 2020Quote: ... mOSE cells (1×10^6) were first plated as monolayer cultures 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). Four days after the addition of TGFB1 ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Immunology 2019Quote: ... anaesthetized C57BL/6 mice were first injected with 50 µl anti-LYVE1-Alexa Fluor 488 antibody (20 µg/ml, R&D Systems) into the rear right hock ...
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bioRxiv - Biochemistry 2019Quote: ... Cytokines (IL-6 and KC) were quantified by an enzyme-linked immunosorbent assay (Quantikine; R&D Systems Europe, Abingdon, Oxon, United Kingdom).
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Immunology 2021Quote: Mature macrophages (n = 6 donors) were stimulated for 48 hours with complete medium containing IFN-γ (20 ng/mL, R&D Systems) and LPS (100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... The expression of PD-L1 and IL-6 from bronchoalveolar lavages was quantitated following the protocols of ELISA kits: Human IL-6 Quantikine ELISA Kit and Human/Cynomolgus Monkey B7-H1/PD-L1 Quantikine ELISA Kit from R&D Systems.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and the supernatant was used for the quantification of IL-1β e IL-6 cytokines using commercial kits (R&D System®).
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit, R&D Systems). All media was collected two days following media change ...
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bioRxiv - Pathology 2020Quote: ... recombinant human Cadherin 6 Fc chimera protein (~130 kD) or recombinant mouse Cadherin 6 protein (~90 kD) was used to generate a standard curve (R&D Systems catalog # 2715-CA or 6826-CA ...
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bioRxiv - Pathology 2020Quote: Cytokines in 200 microgram kidney lysate (pooled from 5-6 samples with equal amount of protein) were detected with the Proteome Profiler Array (ARY028; R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Plasma interleukin (IL)-6 and lipopolysaccharide-binding protein (LBP) levels were measured by commercially available enzyme-linked immunosorbent assay (R&D Systems, Minneapolis ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
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bioRxiv - Microbiology 2022Quote: Distal ileal TNF-α and IL-6 were quantified using the mouse TNF-α enzyme-linked immunosorbent assay [ELISA] Ready-SET-Go kit (eBioscience; San Diego, CA, USA) and the Mouse IL-6 DuoSet ELISA (R&D Systems) respectively ...
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Developmental Biology 2022Quote: Cells were dissociated with TrypLE on day 6 and 8 of CMEC and PMEC protocol and stained with VE-Cadherin PE-conjugated Antibody (R&D Systems). Then VEC+ cells were sorted using FACSAria III ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Mice were sacrificed 24h post-infection and n=7 were analysed for bacterial load and IL-6 and KC using ELISA (R&D systems). A further n=3 animals were analysed using histology ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...