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Citations for R&D Systems :
201 - 250 of 3632 citations for 6 Chloro 4 hydroxy 3 methoxycarbonyl 2H thieno 2 3 e 1 2 thiazine 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... a total of 1 × 106 RAW264.7 or primary peritoneal macrophages were washed with FACS buffer containing PBS with 2% FBS and stained with APC anti-mouse TREM-1 Ab (clone: 174021, R&D systems). Unstained cells were used as a negative control to establish the flow cytometer voltage setting ...
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bioRxiv - Immunology 2020Quote: ... The nitrocellulose membrane was blocked in 5 % non-fat milk in PBS-T for 2 hours at room temperature and probed overnight with goat anti-hACE2 primary Ab at 1 μg/mL (AF933, R&D systems). Following three wash intervals of 10 minutes with PBS-T ...
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bioRxiv - Developmental Biology 2019Quote: ... and 100 ng/ml CHO-derived mouse R-spondin 1 or 5 ng/ml CHO-derived mouse R-spondin 2 (R&D System). The final concentration of Rspondins in the culture medium was initially tested in pilot experiments in order to add comparable amounts of bioactive ligands (bioactivity measured by TOPflash assays and organoid growth curves ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were exposed to 1 µM EdU along with or withpout 2-100 ng/ml recombinant VEGFA (R&D Systems, 493-MV) for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Neuroscience 2022Quote: ... VEGFR-1 and VEGFR-2 protein levels were quantified with the DuoSet® ELISA Development System Kit (R&D Systems, Abingdon, UK), according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... or 4 µg mL-1 human PDGFR beta antibody (AF385, R&D Systems) and 10 µg mL-1 human/mouse/rat alpha-smooth muscle actin antibody (MAB1420 ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ng/mL human bone morphogenetic protein 4 (Bmp4) (R&D Systems, Inc.), 20 ng/mL human basic fibroblast growth factor (b-FGF ...
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bioRxiv - Immunology 2019Quote: Pediatric PBMCs were stimulated for 6 h with (1) TNF (20 ng/ml, R&D Systems), (2 ...
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bioRxiv - Bioengineering 2022Quote: ... angiopoietin-1 (ANG-1, R&D Systems) and hepatocyte growth factor (HGF ...
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bioRxiv - Cell Biology 2022Quote: ... NKX6-1 (1:1000; R&D Systems), NR4A2 (NURR1 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ng/mL mFGF2 (R&D Systems), 1xITS (Millipore Sigma)) ...
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bioRxiv - Bioengineering 2020Quote: ... cleaved caspase-3 (R&D System, MAB835) (1:150 in goat serum ...
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bioRxiv - Genomics 2022Quote: ... 3 µM CHIR99021 (R&D Systems 4423), and 1 µM PD0325901 (Sigma PZ0162) ...
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bioRxiv - Immunology 2021Quote: ... or recombinant ficolin-3 (R&D systems) or human purified C1q (Comp Tech ...
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bioRxiv - Immunology 2020Quote: ... IFNλ2/3 ELISA (R&D Systems DY1789B) and mouse IFNβ ...
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bioRxiv - Cell Biology 2021Quote: ... syndecan-3 (R&D Systems Cat#: FAB3539A) and syndecan-4 (R&D Systems Cat# ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μM CHIR99021 (R&D Systems 4423), and 1 μM PD0325901 (Sigma PZ0162) ...
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bioRxiv - Genomics 2023Quote: ... 3 μM CHIR99021 (R&D Systems 4423) was added to the media ...
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bioRxiv - Immunology 2023Quote: ... anti-cleaved caspase-3 (R&D Systems), anti-RIPK3 (Abcam,) ...
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bioRxiv - Genomics 2023Quote: ... 3 μM CHIR99021 (R&D Systems 4423), and 1 μM PD0325901 (Sigma PZ0162) ...
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bioRxiv - Genetics 2023Quote: ... 10ng/ml IL-3 (R&D systems) and Ls513 cells were cultured in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... 3 ng/mL GMCSF (R&D systems) and 200 ng/mL Flt3-L (R&D systems ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
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bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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bioRxiv - Physiology 2020Quote: ... 0.1 mL/10 g body weight) on day 1 and leptin (2 mg/kg; 0.1 mL/10 g body weight; CAT# 498-OB, R&D Systems, Minneapolis, MN) on day 2 at 6am ...
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bioRxiv - Molecular Biology 2022Quote: Human recombinant BMP-2 and BMP-4 were purchase from R&D Systems (Minneapolis, MN). Anti-Myc and anti-HA antibodies were purchased from Cell Signaling Technology (Danvers ...
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bioRxiv - Molecular Biology 2020Quote: ... Rapamycin (Cat# 1912) and 3-Methyladenine (3-MA, Cat# 3977) were purchased from R&D Systems (Minneapolis, MN, USA). HSC70 (HSPA8 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Immunology 2021Quote: ... and 1 μg/mL rat anti-mouse IL-4 from R&D Systems (MAB404100) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies used were anti-IL6 (R&D Systems, 1:100; overnight 4°C), anti-nucleolin (Santa Cruz ...
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bioRxiv - Bioengineering 2023Quote: ... 10 ng mL− 1 BMP-4 (human recombinant) (R&D Systems, Minneapolis, MN, USA), 10 µM LY294002 (Cayman Chemical ...
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bioRxiv - Developmental Biology 2022Quote: ... Jagged 1 (AF599, R&D systems, 1:500), Dlk1 (AF1144 ...
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bioRxiv - Physiology 2023Quote: ... VCAM-1 (AF643, 1:1000, R&D Systems), BNP (ab19645 ...
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bioRxiv - Immunology 2023Quote: ... and 50 U/mL Interleukin-2 (IL-2, R&D Systems). Pre-isolated NK cells were thawed and co-cultured at a 1:1 effector:target (E:T ...
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bioRxiv - Cell Biology 2022Quote: ... Type 2 (BME-2) droplets (R&D Systems, Minneapolis, MN, USA) and covered under medium ...
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bioRxiv - Microbiology 2021Quote: ... 10ng/ml interleukin 4 (IL-4) and 10ng/ml transforming growth factor beta 1 (TGFβ1) (R&D systems, Minneapolis, MN), and used between days 7-9 of differentiation.
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Immunology 2021Quote: ... at 3 μM and necrosulfonamide (R&D systems) at 1 μM ...
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bioRxiv - Genomics 2021Quote: ... or 3 ng/ml TGFB (R&D Systems), or both ...
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bioRxiv - Immunology 2022Quote: ... anti-IFN-λ2-3 (MAB17892; R&D systems) and anti-IFNAR1 (BE00241 ...