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Citations for R&D Systems :
2351 - 2400 of 4725 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... rmICAM-1 (ICAM-1 Fc chimera; 15µg mL−1; R&D Systems), and rmCXCL1 (15µg mL−1 ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Human serum GzmA levels from healthy donors and patients with confirmed abdominal sepsis at diagnose and during the first 72h was monitored by a commercial ELISA kit (Human Granzyme A DuoSet ELISA, R&D Systems) following the manufacturer recommendations.
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bioRxiv - Developmental Biology 2020Quote: ... An NRG1-β1 ELISA assay was performed according to the protocol of the manufacturer (Human NRG1-β1 DuoSet ELISA, R&D systems). Absolute NRG1-β1 concentration was calculated based on the standard curve.
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bioRxiv - Genomics 2019Quote: ... Serum IL-6 levels were measured using the Quantikine® Enzyme Linked Immuno-Sorbent Assay Human IL-6 Kit (R&D Systems) following the manufacturers standard protocol ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Microbiology 2021Quote: The relative levels of selected human cytokines and chemokines was determined in parallel using a membrane-based antibody array (ARY005B, R&D Systems). Cells were seeded at 7.5 × 105 cells per well in a tissue-culture treated six-well plate ...
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bioRxiv - Neuroscience 2021Quote: ... Protein expression for the expressed human LTα transgene was measured in snap-frozen rat CSF using a human TNF-beta/TNFSF1 Quantikine ELISA kit (DTB00, R&D systems) as per manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Hippocampal homogenate samples were used to determine NRG1 levels with the Human NRG1-β1 ELISA (DY377, R&D Systems, Minneapolis MN, USA). Samples were diluted in kit assay buffer (1:6 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Cell Biology 2021Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: Phosphorylation of several kinases and expression levels of matrix metallopeptidases (MMPs) were analyzed using Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) and Human MMP Antibody Array Kit (Abcam) ...
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bioRxiv - Microbiology 2020Quote: Cells were stimulated for 16 h prior to infection in complete medium at 37°C with addition of 50 IU/mL human IFNγ (285-IF, R&D Systems). Induction of Doxycycline-inducible cells was performed with 200 ng/mL Doxycycline overnight (D9891 ...
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bioRxiv - Systems Biology 2021Quote: ... washed once with FACS buffer (1%BSA in PBS and 5mM EDTA) and incubated for 40 minutes in FACS buffer containing 0.75ug of human ACE2-488 conjugated antibody (R&D Systems FAB9332) and 1:1000 7-AAD (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The secretome for each specimen was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (Ary007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
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bioRxiv - Cell Biology 2020Quote: Relative phosphorylation levels of 43 kinases and 2 related proteins were assessed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... conditioned medium was collected and the levels of secreted human CXCL8 and murine CXCL1 were detected with DuoSet ELISA (R&D systems) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of IL11 in equal volumes of cell culture media were quantified using Human IL11 Quantikine ELISA kit (D1100; R&D Systems). Both assays were performed according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...
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bioRxiv - Bioengineering 2022Quote: Cytokines were measured in the supernatants from ASC cultures and ASC-enriched patches using the human cytokine base kit A (R&D Systems) combined with a magnetic Luminex assay (Bio-plex 200 ...
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bioRxiv - Immunology 2022Quote: ... cells were harvested and transferred to a 24 well plate and cultured in the presence of human rIL-2 (10U/ml, R&D Systems) for 4 additional days ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: Levels of human IL-1β (DY201) and IL-18 (DY318) were measured in the cell supernatants using ELISA kits from R&D Systems. Human IL-6 (# 88-7066-86 ...
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bioRxiv - Microbiology 2021Quote: ... Barrier integrity was further assessed using the Human E-cadherin Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN). Absorbance was measured at 450 nm using a BioTek Synergy H2 plate reader and concentrations of E-cadherin were determined against a standard curve according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG HRP-conjugated Ab (HAF016) from R&D system; mouse anti-HA mAb (16B12) ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in the media (RPMI1640 +2% FBS) with human anti-Nectin-2 monoclonal antibody or isotype control (20 μg/mL, both R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Cancer Biology 2022Quote: Changes in kinase phosphorylation upon exposure to the SASP was assayed using the Proteome Profiler Human Phospho-Kinase Array (R&D Systems). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The supernatants from the serovar D infections were subjected to multiplex ELISA analyses in triplicate setting using a custom designed 27-plex human magnetic Luminex assay #LXSAHM (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total CREB and pCREB were measured using the Human/Mouse/Rat Phospho-CREB (S133) Cell-based ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Site specific phosphorylation levels of 43 kinases were measured using the Human Phospho-Kinase Array Kit (catalog no. ARY 003B, R&D Systems) according to the manufacturer’s instructions with the experimental design as described below ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteome Profiler Human Apoptosis Array kit (CAT# ARY009) and Pepstatin A aspartic protease inhibitor (#1190/10) were purchased from R&D Systems. The cOmplete protease inhibitor cocktail (CAT# 11697498001 ...
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bioRxiv - Cell Biology 2020Quote: ... For TGF-β1 detection in condition media from HPC treated with different experimental conditions and in urine samples from mice and human were analyzed by TGF-β1 ELISA commercial kit (R&D Systems) according to the man-ufacturer’s protocol.
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... The following antibodies were purchased and used for immunostaining of human sample using staining protocols similar to those used in mouse sections: CD31 (AF806, R&D systems), CXCR4 (2B11 ...
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bioRxiv - Developmental Biology 2019Quote: ... Human iPSC generated in this study were characterized for their differentiation potential into the three germ layers using Human Three Germ Layer Kit (R&D systems),according to the manufactures’ instructions with minor modifications ...
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bioRxiv - Immunology 2020Quote: Titration of blocking antibodies was performed by using static adhesion assays in which activated CD4+ T cells were allowed to adhere to either recombinant human ICAM/Fc or VCAM/Fc chimeras in the presence of blocking antibodies specific for integrin α4 (clone 2B4, R&D Systems) or integrin αL (clone TS1/22 ...
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bioRxiv - Cell Biology 2019Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Cxcl10 and Ccl22 in mouse peritoneal cells were analyzed by ELISA according to the manufacturers instructions (Human and Mouse DuoSet ELISA respectively, R&D Systems). For relative chemokine concentration in comparison to parasite load ...
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bioRxiv - Cell Biology 2020Quote: ... and chondrogenic differentiation experiments were performed following the instructions of the human mesenchymal stem cell functional identification kit (R&D systems, SC006). For osteogenic differentiation ...