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Citations for R&D Systems :
2301 - 2350 of 3306 citations for Human Phosphatidic Acid Phosphatase Type 2A PPAP2A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... IL-6 concentrations in maternal blood plasma (3 hours post-injection) were determined using a rat-specific ELISA DuoSet (R&D Systems, Abingdon, UK) as reported elsewhere [27] ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were harvested at the 24h post-treatment time point and analyzed for cytokine content using ELISA for IL-6 (R&D Systems; Minneapolis, MN) according to the manufacturer’s protocol
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bioRxiv - Immunology 2020Quote: Secreted IL-1b and MCP-1 proteins in supernatants of monocytic cells stimulated with TNF-α were quantified using sandwich ELISA following the manufacturer’s instructions (R&D systems, Minneapolis, USA).
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bioRxiv - Immunology 2019Quote: ... and the tissue levels of myeloperoxidase (MPO) in the supernatants were determined using a mouse MPO ELISA (R&D systems, Biotechne, Minneapolis, MN, USA). The fixed tissues (left lungs ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Neuroscience 2022Quote: ... we determined the amount of HB-EGF per cell through enzyme linked immunosorbent assay (ELISA; Cat# DY8239-05, R&D Systems, Minneapolis, MN, USA). For analysis of HB-EGF levels in brain tissue ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and WNT16 levels were determined in senescent fibroblast lysates using a standardized sandwich Enzyme-Linked Immunoassay (ELISA) technique (R&D Systems, Minneapolis, MN, USA), according to manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: ... Then the tumor necrosis factor alpha (TNFA) and interleukin 10 (IL10) protein levels in the tissue were measured by enzyme linked immunosorbent assay (ELISA) (R&D Systems, Abingdon, UK).
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bioRxiv - Immunology 2023Quote: ... cell culture supernatants or sulcus fluid from patients with different disease stage were evaluated for MMP-9 content using ELISA Duo Set (DY911, R&D System, Wiesbaden, Germany.) following the manufactureŕs protocol.
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested to measure the release of IFN-γ using a IFN-γ-specific ELISA according to the manufacturer’s instructions (R&D Systems, DY28B05). To evaluate T cell activation markers ...
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bioRxiv - Bioengineering 2023Quote: ... IP-10/CXCL10 release from mouse cells was assayed using the Quantikine Mouse IP-10 ELISA assay (R&D Systems, Catalog No. DY466-05) using cell-free supernatants of stimulated cells according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... 50 µL of murine plasma was then used to quantify glucagon concentrations with ELISA by following the manufacturer’s protocol (R&D Systems, cat no. DGCG0). Final glucagon concentrations were calculated based on a standard curve.
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bioRxiv - Molecular Biology 2024Quote: ... The secretion of IFN-γ was also quantified in cell supernatants using a sandwich mouse IFN-γ Duo Set ELISA (DY485-05, R&D systems). Standards and samples were diluted appropriately to obtain results within the stated detection levels.
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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bioRxiv - Genomics 2022Quote: ... samples were thawed and cleared by centrifugation for 5 minutes at 1000xg and assayed for urine MMP7 and MMP9 by ELISA according to manufacturer’s instructions (R&D Systems, DMP700/DMP900, Minneapolis, MN). Urine creatinine was used to normalize MMP7 and MMP9 levels between non-active ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Cell Biology 2019Quote: ... 5.0 × 105 PCs and FBs were grown in a single well of a 24-well dish for 24 hours and enzyme-linked immunosorbent assay (ELISA) was performed per manufacture protocol (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Molecular Biology 2020Quote: Hyaluronic acid of COVID-19 patients’ plasma was measured in 1:5 dilution by the enzyme-linked sandwich assay Hyaluronan DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) following the manufacturer’s descriptions ...
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bioRxiv - Pathology 2020Quote: ... TNF-α and IL-1β) in the frozen distal small intestine and colon were measured by porcine specific ELISA assays (R&D Systems, Abingdon, UK).
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bioRxiv - Physiology 2019Quote: ... the clones were assayed for IFN-γ secretion in a peptide-specific manner following their co-incubation with T2 cells pulsed with MART-126–35 (commercially synthesized and purified [>95%] by reverse-phase HPLC by Biomer Technology, Cheshire, UK) by ELISA (R&D Systems, Minneapolis, MN, USA). The MART-126–35–-reactive clones were further expanded in a second-round of exposure to 30 ng/ml anti-CD3 ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected 24 hours later and stored at -80 °C until analysis of TNF-⍰ levels via ELISA (DY210; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were resuspended in Goat anti-Human ACE2 antibody (1:500, R&D Systems) in the flow buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with recombinant human IFNγ (R&D Systems, 285-IF-100) by adding an equal volume of the complete culture media containing the cytokine ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant FGF2 (10 ng/ml, R&D Systems, Minneapolis, Minnesota; 233-FB). After two to three weeks ...
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bioRxiv - Cell Biology 2020Quote: ... Gas1 was stained using goat anti-human Gas1 antibodies (R&D systems, Cat# AF2636). Dhh was stained using mouse anti-Dhh antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human TGF-β (7754-BH) was purchased from R&D Systems (Minneapolis, MI). It was reconstituted at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used: Human alpha-Smooth Muscle Actin (R&D Systems, MAB1420), Human/Mouse alpha-Fetoprotein/AFP (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... during 2 hours and stimulated with human recombinant VEGF165 (25ng/ml, R&D System) during 30 minutes.
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bioRxiv - Genomics 2019Quote: ... 50 ng/mL carrier-free recombinant human stem cell factor (SCF; R&D Systems), 50 ng/mL TPO (R&D Systems) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 100ng/mL human recombinant FGF4 (R&D Systems, 235-F4) in the presence of 10 μg/mL heparin sulfate and 3 μM BI-D1870 RSK inhibitor at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... PDGF-AB were determined by Human Magnetic Luminex Assays (R&D Systems, Minneapolis, MN) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... NMPM: N2B27 supplemented with 40 ng/ml recombinant human (rh) FGF2 (R&D Systems), 40 ng/ml rhFGF8 (R&D Systems) ...
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bioRxiv - Biophysics 2019Quote: CCL5/RANTES was included in Human Chemokine Protein Sampler Pack from R&D systems..
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bioRxiv - Cell Biology 2020Quote: ... were incubated with 100μg/ml recombinant human Wnt10b protein (0.1mg/ml;R&D Systems) at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Human recombinant pro-legumain (asparaginyl endopeptidase) was purchased from R&D Systems (2199-CY), activated per manufacturer instruction and active site titrated using MP-L01 inhibitor as described by Poreba et al ...
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bioRxiv - Bioengineering 2019Quote: ... 0.004 % of recombinant human FGF basic (FGF-2, R&D Systems, Abingdon, United Kingdom) was added ...
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bioRxiv - Neuroscience 2019Quote: Recombinant human IDO (Accession #P14902) was obtained from R&D Systems (#6030-AO-010) and frozen at −80°C until use ...
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bioRxiv - Cancer Biology 2019Quote: ... Recombinant human TGFβ-1 (cat# 240B002) was purchased from R&D systems (Minneapolis, MN). Alexa 488-conjugated phalloidin (cat# A12379 ...
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bioRxiv - Immunology 2021Quote: ... recombinant human IL-4 at 50 ng/ml (R&D Systems, Minneapolis, MN, USA) for 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... the following antibodies were used: anti-human Nestin (R&D Systems, MAB1259, 1:2,400), goat anti-TrkB (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant mouse interferon-γ and recombinant human interkeukin-8 (R&D Systems, Minneapolis, MN) was prepared in PBS containing 0.1% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... then labelled with and without anti-human LILRB2 (5 µg/mL; R&D Systems). The cells were washed with PBS (0.5% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... and Human chitinase 3-like 1 (YKL-40) were purchased from R&D Systems. Bovine serum albumin was purchased from Sigma Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... and recombinant human IL-2 (50 U/mL, R&D Systems, Minneapolis, MN, USA). Single cell suspensions were activated with 1 μg/ml Concavalin A (Sigma ...
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bioRxiv - Biochemistry 2020Quote: The mouse and human anti-CHL1 antibodies (AF2147 and MAB2126; R&D systems, MN) were partially reduced and bound to HRP using a peroxidase labeling kit SH (Dojindo ...