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Citations for R&D Systems :
2251 - 2300 of 5024 citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Primary neurons and EVs were pre-incubated with active recombinant human CD59 (cat. no. 1987-CD, R&D Systems) at a final concentration of 1 μM (stock at 10 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... FGF-2 in the CM was assayed using a human FGF basic DuoSet ELISA Development Kit (R&D Systems) according to the manufacturer’s procedure.
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bioRxiv - Neuroscience 2019Quote: ... Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution) using DuoSet ELISA kits (R&D Systems). DuoSet ELISA Ancillary Reagent Kit 2 was used in conjunction with all DuoSet ELISA kits ...
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bioRxiv - Immunology 2019Quote: ... and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems, Ref. HS600C Lot P178994). All these immunoassays were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... TNF-α was measured using a Quantikine HS ELISA Human TNF-α (R&D Systems, Ref. HSTA00E Lot P174731) and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... CXCL8 and Osteopontin (SPP1) proteins in conditioned media was performed using a custom human luminex assay (R&D systems), according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2020Quote: Phosphorylation status and total amounts of 43 kinases were measured using Human Phospho-Kinase Array from R&D Systems. 1.5×106 LN229 cells were stimulated with 400 ng/mL of GAS6 for 10 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... with 10% FBS (Wisent) and 0.5 ng/ml recombinant human Macrophage Colony Stimulating factor (M-CSF) (R&D Systems) to allow for differentiation of monocytes into macrophages ...
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bioRxiv - Bioengineering 2020Quote: ... 97 µL of urine was incubated with 1 µL of human cathepsin B (R&D Systems, 953-CY-010) and 1 µL of DTT for 15 minutes to inactivate the urine cysteine protease inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
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bioRxiv - Physiology 2021Quote: ... The medium was supplemented with 100 ng/mL recombinant human M-CSF using CellXVivo™ kit (R&D Systems) and differentiated for 5–7 days prior to use.
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against human DDR1 (R&D Systems, AF2396), CXCL5 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with 10 ng ml−1 of recombinant human IFNγ (R&D Systems, 285-IF-100), 10 ng ml−1 of recombinant human TNFα (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... For the IL-8 neutralization experiments: 0.4 μg/ml of human IL-8 monoclonal antibody (R&D Systems, USA) was added to the apical and basolateral compartment of enteroid monolayers and to the PMN suspensions before they were co-cultured ...
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bioRxiv - Immunology 2022Quote: Human IL-8 was measured from cell culture supernatants using commercially available specific paired ELISA kits (R&D Systems). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... IFN-β amounts in the culture supernatant were measured by human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2021Quote: ... The membranes were incubated with a sheep polyclonal antibody to human DG (AF6868; R&D Systems, 1:100 dilution) and a mouse monoclonal antibody to a glycoepitope on the sugar chain of α-DG (IIH6 ...
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bioRxiv - Biochemistry 2021Quote: For TCR-mediated adhesion 96-well plates were pre-coated with 0.5μg human ICAM-1/well (R&D systems). Transfected cells were left untreated or stimulated with anti-TCR antibody (OKT3 ...
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bioRxiv - Physiology 2022Quote: ... Aβ ELISA was performed using a human amyloid β (aa1-42) immunoassay Quantikine ELISA kit (R&D Systems, Inc.).
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bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
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bioRxiv - Molecular Biology 2022Quote: ... human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF, 50 ng/mL R&D Systems, 7954-GM-020/CF), and interleukin-4 (IL-4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of GDF15 was measured using an ELISA assay for Human GDF-15 DuoSet (R&D systems, # DY957) as per the manufacturer’s instructions on a Tecan INFINITE® 200 Pro spectrophotometer ...
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bioRxiv - Immunology 2020Quote: ... was titered using a commercial enzyme-linked immunosorbent assay kit (Human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml−1 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Synthetic Biology 2021Quote: ... cell-free supernatant was collected and quantified using the Human IL-12p70 DuoSet ELISA (R&D Systems; catalog# DY1270), as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Osteoclastogenesis was induced by treatment with 25 ng/ml human M-CSF (216-MC, R&D Systems, Abingdon, UK) and 30 ng/ml RANKL (310-01 ...
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bioRxiv - Immunology 2020Quote: ... and 10 ng/mL of human macrophage colony stimulating factor (M-CSF, R&D Systems, catalog number 216-MC) for at least 10 days to generate mature macrophages before use in experiments.
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bioRxiv - Immunology 2021Quote: ... The concentration of sCD25 was measured with the Human CD25/IL-2R alpha Quantikine ELISA kit (R&D Systems) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The binding of pig and rabbit (Thymoglobulin®, Sanofi) IgG to human FcγRIIIa (CD16a, R&D Systems, 4325-FC) receptor was also measured by SPR ...
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bioRxiv - Cell Biology 2021Quote: ... NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems, USA) and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... secreted matrix metalloproteinases (MMP) concentrations were measured using the magnetic Human MMP Magnetic Luminex Performance Assay (R&D Systems). This assay required dilutions of cell culture supernatants in fresh culture medium at either 2x or 200x to detect the reported analytes within a quantifiable range.
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bioRxiv - Molecular Biology 2022Quote: ... Day 0: 10 ng/ml recombinant human FGF basic/FGF2/bFGF (146 aa) protein (R&D Systems 233-FB), 10 µM RI (what is RI?) ...
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bioRxiv - Bioengineering 2022Quote: ... Enzyme-Linked Immunosorbent Assay (ELISA) kit (DuoSet ELISA Ancillary Reagent Kit and Human Lubricin/PRG4 kit, R&D systems) was used following manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cell supernatants were then harvested and assayed for cytokines by Human Cytokine Array (Proteome Profiler, R&D systems, ARY005B), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL recombinant human glial cell line-derived neurotrophic factor (GDNF, #212-GD-010, R&D Systems, Germany), 500 µM cAMP (#D0260 ...
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bioRxiv - Physiology 2023Quote: ... Diluted serum was then used to measure human HB-EGF with the DuoSet ELISA Kit (DY259B, R&D Systems).
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected and analyzed for the presence of secreted factors using Luminex human Discovery Assays (R&D Systems): LXSAHM-02 (MMP-8) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... each well was then filled with 25 μL/well recombinant human TNFα (R&D systems, cat #: 210-TA-100) diluted in cell culture medium (final concentration 10 ng/mL ...
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bioRxiv - Neuroscience 2022Quote: ... The Human Aβ1-42 in soluble and insoluble fractions were qualified with Quantikine ™ ELISA kit (R&D Systems) based on the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... in absence or presence of stimuli (PMA/ionomycin or human recombinant soluble BAFF/BLyS/TNFSF13B Protein (R&D System)) for 18 h at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cytokine array measurements were made using the Proteome Profiler Human XL Cytokine Array Kit (ARY022B) from R&D systems, following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The differentiated organoids were treated with the indicated doses of recombinant human IL-1β (R&D system, 201-LB) for an additional 48 hrs.
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bioRxiv - Bioengineering 2023Quote: ... and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Biophysics 2023Quote: ... and then a binding buffer solution containing 200 nM of recombinant αVβ5 human integrin extracellular domains (R&D Systems) was slowly added to one of the wells of the fluid chamber ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and IL6 releases were analysed by Luminex® Assay using the Human Premixed Multi-Analyte Kit (R&D Systems) according to manufacturer instructions and acquiring readings with a BioPlex MagPlex Magneti Beads system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: MSCs were stimulated with recombinant human IFNα (0.03 U/ml) or IFNβ (60 pg/ml) protein (R&D Systems), in combination with different concentrations of inhibitor of IFNα/β (i-IFNα/β ...
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bioRxiv - Neuroscience 2023Quote: ... and B2M in collected media were examined using Human Magnetic Luminex® Assays (R&D Systems; Cat. # LXSAHM-03) according to the manufacturer’s instructions and read on a MAGPIX instrument (Luminex Corp).
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h ...