Labshake search
Citations for R&D Systems :
2201 - 2250 of 2473 citations for Recombinant Human SFN GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Purified mouse monoclonal IgG anti-human interferon-γ capture antibody (Cat. #MAB2852, lot #FIO1022021, R&D Systems, Minneapolis, Minnesota, United States), biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl biotinylated rhIL-2 or biotinylated control protein (from Fluorokine® Biotinylated Human IL-2 Kit, R&D Systems) added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... were incubated for 2 h at room temperature (RT) with biotinylated polyclonal goat anti-human TREM2 capture antibody (0.1 μg/ml; AF1828, R&D Systems). Following incubation with streptavidin-HRP (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Supernatants harvested from infected cells were assayed using enzyme-linked immunosorbent assay (ELISA) kits for human IL-18 (R&D Systems) and IL-1β (BD Biosciences).
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bioRxiv - Immunology 2023Quote: ... HUT78 cells were stimulated in complete IMDM media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems). All treatments were 24 hours.
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bioRxiv - Immunology 2023Quote: ... K562 cells were stimulated in complete RPMI media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems), 20 ng/mL human IFN-β (84-99I-F010 ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ levels were measured in NK cell media samples using the human IFN-γ DuoSet ELISA kit (R&D Systems). IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... IL-6 levels were measured in B cell media samples using the human IL-6 DuoSet ELISA kit (R&D Systems) (53 ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: The concentration of some of the plasma markers was measured by ELISA using Human sCD14 DuoSet ELISA (R&D Systems, DY383) and Human FABP2/I-FABP DuoSet ELISA (R&D Systems ...
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bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
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bioRxiv - Neuroscience 2023Quote: Serum levels of Fgf2 were assessed with the Quantikine® Human FGF2 ELISA kit from R&D systems (Cat. No. DFB50), following manufacturers instructions ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were cultured for 8 days and stimulated with 2.5 ng/mL crude peanut extract (Stallergenes Greer) with or without 50 ug/mL anti-human IL-4Ra (R&D Systems), as previously described (17).
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bioRxiv - Neuroscience 2023Quote: ... Activin A levels were measured using an ELISA kit (Human/Mouse/Rat Activin A Quantikine ELISA Kit, R&D Systems, DAC00B).
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bioRxiv - Cell Biology 2023Quote: Angiogenic regulators profiled in the conditioned medium were analyzed using a Proteome Profiler Human Angiogenesis Array Kit (ARY007; R&D Systems). The conditioned medium (1 mL ...
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bioRxiv - Cancer Biology 2023Quote: ... and S100A8/A9 concentrations were determined using the human S100A8/A9 heterodimer ELISA kits (R&D Systems, DS8900, and DY8226-05) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Genetics 2021Quote: ... Cell culture supernatants were collected and assayed for the secretion of pro-Col1A1 using Human Pro-Collagen I alpha 1 DuoSet ELISA kit (DY6220-05, R&D Systems) as described by the manufacturer.
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Human serum GzmA levels from healthy donors and patients with confirmed abdominal sepsis at diagnose and during the first 72h was monitored by a commercial ELISA kit (Human Granzyme A DuoSet ELISA, R&D Systems) following the manufacturer recommendations.
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bioRxiv - Developmental Biology 2020Quote: ... An NRG1-β1 ELISA assay was performed according to the protocol of the manufacturer (Human NRG1-β1 DuoSet ELISA, R&D systems). Absolute NRG1-β1 concentration was calculated based on the standard curve.
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bioRxiv - Genomics 2019Quote: ... Serum IL-6 levels were measured using the Quantikine® Enzyme Linked Immuno-Sorbent Assay Human IL-6 Kit (R&D Systems) following the manufacturers standard protocol ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Microbiology 2021Quote: The relative levels of selected human cytokines and chemokines was determined in parallel using a membrane-based antibody array (ARY005B, R&D Systems). Cells were seeded at 7.5 × 105 cells per well in a tissue-culture treated six-well plate ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated for 16 h prior to infection with addition of 50 IU mL-1 human IFNγ (285-IF, R&D Systems). Induction of GBP expression in the Dox-inducible cells was performed with 200 ng mL-1 Dox overnight (D9891 ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Neuroscience 2021Quote: ... Protein expression for the expressed human LTα transgene was measured in snap-frozen rat CSF using a human TNF-beta/TNFSF1 Quantikine ELISA kit (DTB00, R&D systems) as per manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Hippocampal homogenate samples were used to determine NRG1 levels with the Human NRG1-β1 ELISA (DY377, R&D Systems, Minneapolis MN, USA). Samples were diluted in kit assay buffer (1:6 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: Phosphorylation of several kinases and expression levels of matrix metallopeptidases (MMPs) were analyzed using Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) and Human MMP Antibody Array Kit (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... cells were wash three times with PBS to remove any residual FBS and followed by 1-hour treatment with goat anti-human ACE2 antibody (R&D Systems) or anti-hDDP4 antibody (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: Cells were stimulated for 16 h prior to infection in complete medium at 37°C with addition of 50 IU/mL human IFNγ (285-IF, R&D Systems). Induction of Doxycycline-inducible cells was performed with 200 ng/mL Doxycycline overnight (D9891 ...
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bioRxiv - Systems Biology 2021Quote: ... washed once with FACS buffer (1%BSA in PBS and 5mM EDTA) and incubated for 40 minutes in FACS buffer containing 0.75ug of human ACE2-488 conjugated antibody (R&D Systems FAB9332) and 1:1000 7-AAD (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The secretome for each specimen was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (Ary007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
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bioRxiv - Cell Biology 2020Quote: Relative phosphorylation levels of 43 kinases and 2 related proteins were assessed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... conditioned medium was collected and the levels of secreted human CXCL8 and murine CXCL1 were detected with DuoSet ELISA (R&D systems) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... Calprotectin levels were determined in optimally diluted (1:20) extracts using Human-S100A8/S100A9 Heterodimer DuoSet-ELISA kit from R&D Systems. Zonulin levels were determined in similarly diluted extracts using human Zonulin Stool ELISA kit from ALPCO ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of IL11 in equal volumes of cell culture media were quantified using Human IL11 Quantikine ELISA kit (D1100; R&D Systems). Both assays were performed according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...