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Citations for R&D Systems :
2201 - 2250 of 2576 citations for Recombinant Human IL12A Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... was titered using a commercial enzyme-linked immunosorbent assay kit (Human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml−1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells surface ACE2 protein was stained with Alex Flour® 647-conjugated human ACE2 antibody (R&D Systems FAB933R). Live/Dead (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The level of human or mouse CXCL10/IP-10 was determined using a DuoSet ELISA kit (R&D Systems) and the level of mouse IL-6 was determined using a DuoSet ELISA kit (R&D systems ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were exposed to the Human/Mouse Brachyury Affinity Purified Polyclonal Goat IgG antibody (R&D Systems, Catalog # AF2085) overnight at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell-free supernatant was collected and quantified using the Human IL-12p70 DuoSet ELISA (R&D Systems; catalog# DY1270), as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Osteoclastogenesis was induced by treatment with 25 ng/ml human M-CSF (216-MC, R&D Systems, Abingdon, UK) and 30 ng/ml RANKL (310-01 ...
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bioRxiv - Immunology 2020Quote: ... and 10 ng/mL of human macrophage colony stimulating factor (M-CSF, R&D Systems, catalog number 216-MC) for at least 10 days to generate mature macrophages before use in experiments.
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bioRxiv - Immunology 2021Quote: ... The concentration of sCD25 was measured with the Human CD25/IL-2R alpha Quantikine ELISA kit (R&D Systems) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... secreted matrix metalloproteinases (MMP) concentrations were measured using the magnetic Human MMP Magnetic Luminex Performance Assay (R&D Systems). This assay required dilutions of cell culture supernatants in fresh culture medium at either 2x or 200x to detect the reported analytes within a quantifiable range.
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bioRxiv - Bioengineering 2022Quote: ... Enzyme-Linked Immunosorbent Assay (ELISA) kit (DuoSet ELISA Ancillary Reagent Kit and Human Lubricin/PRG4 kit, R&D systems) was used following manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cell supernatants were then harvested and assayed for cytokines by Human Cytokine Array (Proteome Profiler, R&D systems, ARY005B), according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... Diluted serum was then used to measure human HB-EGF with the DuoSet ELISA Kit (DY259B, R&D Systems).
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bioRxiv - Microbiology 2022Quote: ... Anti-Human TIM-1 monoclonal antibody (MAB1750) and polyclonal antibody (AF1750) were purchased from R&D systems (Minnesota, USA). Anti-GAPDH polyclonal antibody (sc-25778 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected and analyzed for the presence of secreted factors using Luminex human Discovery Assays (R&D Systems): LXSAHM-02 (MMP-8) ...
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bioRxiv - Neuroscience 2022Quote: ... The Human Aβ1-42 in soluble and insoluble fractions were qualified with Quantikine ™ ELISA kit (R&D Systems) based on the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Cytokine array measurements were made using the Proteome Profiler Human XL Cytokine Array Kit (ARY022B) from R&D systems, following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Human vasculature labeling was performed by using a cocktail of goat anti-podocalyxin (1:20, AF1658, R&D Systems), mouse anti-CD31 (1:50 ...
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bioRxiv - Bioengineering 2023Quote: ... and IL6 releases were analysed by Luminex® Assay using the Human Premixed Multi-Analyte Kit (R&D Systems) according to manufacturer instructions and acquiring readings with a BioPlex MagPlex Magneti Beads system (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with mouse anti-human CCR1 antibody (2.5 µg/106 cells; mab145, R&D Systems) for 20 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and B2M in collected media were examined using Human Magnetic Luminex® Assays (R&D Systems; Cat. # LXSAHM-03) according to the manufacturer’s instructions and read on a MAGPIX instrument (Luminex Corp).
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bioRxiv - Physiology 2024Quote: ... PDGFR-α (goat anti human, 1:300, cat. no.: AF-307-NA; RRID: AB_354459, R&D Systems, Bio-Techne), and C3 (rabbit anti human ...
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bioRxiv - Cancer Biology 2024Quote: ... the conditioned media were assayed with Proteome Profiler Human XL Cytokine Arrays (ARY022B; R&D Systems, Minneapolis, MN, USA) performed as per manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA, R&D Systems) and a decrease of ≥0.3 OD450 value between post and pre-surgery sera was considered significant ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 50 ng/mL human bone morphogenetic protein 4 (hBMP4) (R&D Systems, Minneapolis, Minnesota, USA), 50 ng/mL human vascular endothelial growth factor (hVEGF ...
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bioRxiv - Microbiology 2024Quote: ... The relative levels of cytokines were assessed using the Proteome Profiler Human XLCytokine Array kit (R&D Systems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial suspensions were incubated with human SP-D at a concentration of 10mg/ml (R&D Systems, 1920-SP-050) for 1h at RT without agitation and observed by light microscopy (Nikon Ti Eclipse equipped with a metal-oxide-semiconductor (sCMOS ...
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bioRxiv - Developmental Biology 2021Quote: ... coli-derived human N-terminal modified (C24II) fragment of Sonic Hedgehog (Shh-N; R&D Systems, Cat#1845-SH/CF) was reconstituted in PBS at 10µM and stored at -80°C ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was collected and processed using the Proteome Profiler Human Cytokine Array Kit (R&D Systems; Cat. No. ARY005B), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN 20). To reduce the skewness of phenotype distributions ...
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bioRxiv - Neuroscience 2019Quote: Human TNFα was quantified in blood serum (1:10 dilution) using a DuoSet ELISA kit (R&D Systems, Minneapolis, MN). Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... IL-11 was quantified by using a Human IL-11 Quantikine ELISA Kit according to manufacturer instructions (R&D Systems). Statistical analysis was performed on log10-transformed values with two-way ANOVA tests and Tukey correction ...
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bioRxiv - Bioengineering 2020Quote: ... IL-15 superagonist was quantified using the human IL-15/IL-15R alpha complex DuoSet ELISA (R&D Systems DY6924). For BiTE experiments with primary human T cells ...
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bioRxiv - Neuroscience 2020Quote: ... cells were fluorescent activated cell (FACS)-sorted for human alkaline phosphatase (R&D systems, Minneapolis, MN, USA, Cat N° MAB1448) and expanded further in T75 flasks (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared and analyzed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, cat# ARY003B) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Phospho-kinase screening was performed using a phospho-kinase array (Proteome Profiler Human Phospho-Kinase Array #ARY003B; R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1 ...
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bioRxiv - Physiology 2021Quote: ... CCL2 levels in urine specimens were quantified using Human CCL2/MCP-1 Quantikine® ELISA Kit (R&D systems, DCP00) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cells were characterised using hPSC imaging kit (Human Pluripotent Stem Cell Marker Antibody Panel kit, SC008, R&D system, UK) with analysis performed on a flow cytometer (Beckman Coulter Cytomics FC 500) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were cultured with 1000 U/mL human type I IFN-α2a (MφIFN; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2022Quote: ... 104 sorted CD34+ HSPCs were plated for methylcellulose-based colony forming assays [Human Methylcellulose Base Medium HSC002 (R&D Systems), which were supplemented with 2% streptomycin/penicillin ...
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bioRxiv - Microbiology 2021Quote: ... ELISA was performed using Human Gas6 DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems, Minneapolis, Minnesota, USA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933 ...
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bioRxiv - Cancer Biology 2022Quote: ... Chemokine levels were measured using the Invitrogen ELISA Kit (Quantikine mouse/human CXCL10/IP-10; R&D Systems, Minneapolis, MN) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718, RRID:AB_2278695; R&D Systems, Inc., Minneapolis, MN, USA); and developed using SuperSignalTM west -pico or -femto chemiluminescence kit (Thermo Fisher ...