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Citations for R&D Systems :
2101 - 2150 of 2727 citations for Human IL 17 Receptor since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... were obtained from BD Biosciences. Recombinant human Gal-9 protein (Cat. number 9064-GA) was obtained from R&D systems. SARS-CoV-2 Spike Protein (RBD ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2mM ascorbic acid and 1 ng/mL recombinant human FGF (R&D system, 233-GMP-025). The adherent cells were split by trypsinization at 80% confluency and frozen at passage 2 (50% αMEM ...
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bioRxiv - Immunology 2023Quote: ... After incubation with nitrocellulose membranes from the Human XL Cytokine Array kit (ARY022B, R&D Systems), the cytokine/ detection antibody complexes were bound by their cognate capturing antibodies immobilized on the membranes as spots ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Cells were co-stained with a rabbit anti-human VE-Cadherin (1:100, R&D Systems). Secondary antibodies included Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 546 donkey anti-rabbit IgG (both 1:200 ...
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bioRxiv - Immunology 2024Quote: ... Human/Mouse ROR gamma t/RORC2/NR1F3 PE-conjugated Antibody (1:10, IC6006P, R&D Systems), and Human/Mouse/Rat FoxP3 PE-conjugated Antibody (1:10 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with either BSA or human recombinant WNT3a (5036-WN-010, R&D Systems) 24 hours later ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were treated with recombinant human PD-1 Fc protein (1086-PD-050, R&D System) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat#2028-EG),10 ng/mL human FGF2 (Catalog Number: 233-FB/CF, R&D Systems, Minneapolis, MN, USA), which stimulates the proliferation of murine cells 28 ...
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bioRxiv - Cancer Biology 2024Quote: ... we used Human MIF and midkine DuoSet ELISA kits (DY289 and DY258, respectively; R&D systems). Cells were plated in a 96-well plate and supernatant was collected at several time point ...
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bioRxiv - Cell Biology 2024Quote: Recombinant human TN-C (50 μg) (3358-TC-050; R&D Systems Inc, Minneapolis, MN, USA) was dissolved in 100 μL PBS to produce a stock solution (500 μg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant Human His6-SENP1 Catalytic Domain (SENP1, Catalog no. E-700) was from R&D Systems.
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bioRxiv - Cell Biology 2024Quote: ... Purified human recombinant HGF (100-250 ng/ml, as indicated) was obtained from R&D Systems. Protein-A Gold 10 nm was from Utrecht University ...
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bioRxiv - Microbiology 2024Quote: ... diluted 2X and assayed using a 10-plex Human Luminex Discovery Assay from R&D Systems on a Luminex 100/200 ...
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bioRxiv - Immunology 2021Quote: ... culture supernatants were collected and the IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA) using a commercial kit (R&D Systems, Minneapolis, MN, USA). The supernatants were precipitated with ice-cold acetone and resolved in 1 × Laemmli buffer for Western blot analysis.
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) and incubated at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using the following primary and secondary antibodies: anti-IL-1β (R&D systems, AF-201-NA), anti-GFP (Clontech ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Pathology 2020Quote: ... TNF-α and IL-1β) in the frozen distal small intestine and colon were measured by porcine specific ELISA assays (R&D Systems, Abingdon, UK).
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bioRxiv - Microbiology 2021Quote: ... Memory CD4+ T-cells were infected with HIV-1 (50 ng HIV-p24/106 cells) and then cultured in the presence of IL-2 (5 ng/ml; R&D Systems, Minneapolis, MN, USA) for up to 9 days ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Cell Biology 2021Quote: ... Pro-inflammatory stress was induced using a cytokines mix composed of: IL-1β (50 U/mL) (cat. 201-LB-005, R&D system Minneapolis, MN, USA), TNFα (1000 U/mL ...
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bioRxiv - Immunology 2022Quote: ... then assayed in technical duplicate for secreted chemokines and cytokines by ELISA (IL-15, BAFF, CXCL10/IP-10, and CXCL11/I-TAC, all R&D Systems; CXCL9/MIG, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... TNF-α and IL-1β were measured in the supernatant by ELISA with Mouse TNF-α Quantikine ELISA Kit (R&D Systems MTA00B, Minneapolis MN) and Mouse IL-1β/IL-1F2 DuoSet ELISA Kit according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: FBXW11 WT and FBXW11 KO cell lines (A549 and Ishikawa) were treated with 20 ng/ml rhIL-17A (R&D Systems, #7955-IL-025/CF), rhIL-17B (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Cell Biology 2020Quote: ... we used a proteome profiler for human phospho-kinases (ARY003C, R&D Systems, Biotechne, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... L-glutamine (2mM) and 50 ng/mL of recombinant human M-CSF (R&D Systems, Minneapolis, Minnesota) for 6 to 7 days to differentiate them to macrophages [41] ...
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bioRxiv - Molecular Biology 2021Quote: ... purified 6xHis-Clr4 protein was mixed with human Ubiquitin-activating Enzyme (UBE1, R&D systems E-305), human UbcH5c/UBE2D3 (R&D systems E2-627) ...
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bioRxiv - Immunology 2019Quote: ... Following transfection cells were stimulated with 200 U/ml of recombinant human (h) TNFα (R&D Systems). Luciferase activity was assayed in cell lysates harvested 8 h post-stimulation ...
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bioRxiv - Cancer Biology 2019Quote: ... the phospho-MAPK array assay was performed using the human phospho-MAPK array kit (R&D Systems) according to the provided protocol.
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bioRxiv - Physiology 2019Quote: ... fully differentiated mouse or human brown adipocytes were incubated with 100ng/ml activin B (R&D Systems) in DMEM/F12 supplemented with 10% FBS and 100µg/ml penicillin-streptomycin for 24h before lysis and for RNA extraction of RNA (as indicated below) ...
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bioRxiv - Cancer Biology 2019Quote: Apoptosis array was performed by using Proteome Profiler Human Apoptosis Array Kit (ARY009) from R&D Systems following manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... The concentration of OPN was determined by the Human Osteopontin (OPN) DuoSet ELISA (DY1433, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used to immunostain human tissue: PDGFRβ (1:100, goat, R&D Systems), GFAP (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 cells were stained with Goat anti-Human Phycoerythrin-conjugated ACE2 Polyclonal Antibody (R&D Systems) for 30 min at 4 °C in dark ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL human Fms-related tyrosine kinase 3 ligand (Flt3L, #308-FK, all R&D Systems), 2 mM L-GlutaMAX (Gibco #35050-038) ...
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bioRxiv - Cancer Biology 2020Quote: Cytokine profiling was performed simultaneously using the Human XL Cytokine Array (R&D Systems, ARY022, Minneapolis, MN). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... GDF-15 expression was analyzed using Human GDF-15 DuoSet® ELISA (DY957; R&D Systems™). For the activation of latent TGF-β1 ...
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bioRxiv - Cell Biology 2020Quote: ... USA) and human recombinant lumican protein (lacking GAG chains) was from R&D Systems (Minneapolis, MN, USA). CS sodium salt isolated from bovine cartilage and ChABC from Proteus vulgaris were from Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained 1h at RT with a goat polyclonal anti-human ACE2 (AF933, R&D Systems) diluted at 3μg/ml in PBS + 0.3% triton X-100 + 1% horse serum + 1% BSA + 0,01% sodium azide ...
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bioRxiv - Microbiology 2020Quote: ... The transduced cells were stained with anti-human ACE-2 polyclonal goat IgG (AF933, R&D Systems) primary antibody at 1 ug/mL and donkey anti-goat IgG conjugated to Alexa Fluor 488 (ab150129 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-human sFRP-1 and antihuman Dkk-1 antibodies (cat# CCM017; R&D Systems; Minneapolis, MN, USA) for 15 days ...
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bioRxiv - Cancer Biology 2020Quote: SHP2 phosphatase activity was determined using the human/mouse/rat active DuoSet IC kit (R&D Systems). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates were incubated overnight with nitrocellulose membranes of the Human Phospho-Kinase Array (R&D Systems). Membranes were then washed ...
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bioRxiv - Bioengineering 2022Quote: ... the supernatants were collected and assayed in a Proteome Profiler Human Chemokine Array (ARY017, R&D systems) following manufacturer’s protocol ...