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Citations for R&D Systems :
2051 - 2100 of 4312 citations for GDNF family receptor alpha 1 GFRA1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Parvalbumin (R&D system AF5058, 1:50 – 1:100), cFOS (Santa Cuz s-166940 ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... 1 µM dorsomorphin homolog 1 (4126, R&D Systems), 3 µM CHIR99021 (13122 ...
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bioRxiv - Cell Biology 2020Quote: ... dPSCs were differentiated into each of the three germ layers according to the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems®, Catalog # SC027B).
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS without Ca2+ and Mg2+and incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were subsequently washed with PBS without Ca2+ and Mg2+ and then incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Immunology 2019Quote: ... ELISAs were performed according to the manufacturer’s instructions for IL-10 (Quantikine®HS ELISA Human IL-10 Immunoassay, R&D Systems, Inc., Cat# HS100B), sCD14 (Quantikine® ELISA Human sCD14 Immunoassay ...
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bioRxiv - Cancer Biology 2019Quote: ... For both assays the cells were cultured for 72 hrs at 37 °C and 5% CO2 and Cell free supernatants were then collected and used to perform IFN-γ ELISA using the Human IFN-γ DuoSet assay (R&D system, DY285).
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bioRxiv - Genomics 2020Quote: ... the EBs were dissociated with trypsin-EDTA and reaggregated for 40 hr in serum-free differentiation media with the addition of 8ng/ml human activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R & D Systems Cat# 314-BP) ...
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bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
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bioRxiv - Microbiology 2020Quote: ... Caco2 cells were pre-treated for 30 min at 37°C with goat antibody directed against the human ACE2 or DDP4 ectodomain (R&D Systems, Wiesbaden-Nordenstadt, Germany). Then ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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bioRxiv - Microbiology 2020Quote: ... cell-free supernatants were collected and used to evaluate the concentrations of TGF-β1 with human and rat TGF-β1 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Physiology 2020Quote: ... The measurement was performed by Enzyme-Linked Immunosorbent Assay (ELISA) using the Human PCSK9 Quantikine ELISA kit (R&D Systems, Catalog no. DPC900, Minneapolis, USA) according to kit instructions ...
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bioRxiv - Cell Biology 2022Quote: The relative levels of angiogenesis-related cytokines in the MSC-CM were analysed using the Proteome Profiler Human Cytokine Array Kit from R&D systems (Abingdon, UK, ARY022B) per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Physiology 2024Quote: ... minus 10 nM prostaglandin E2 and 2.5 μM Thiazovinin and with added human recombinant Wnt3A 50 ng/mL (R&D Systems, #5036-WN-010/CF), R-Spondin-1 250 ng/mL (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse tibialis anterior muscles were injected with 50 uL solution of 50,000 human FAPs resuspended in 50% glycerol in PBS with either 29ng/ul concentration of recombinant DLK1 resuspended in PBS (R&D Systems, #1144-PR-025) or with control (identical volume of PBS ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.
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bioRxiv - Cancer Biology 2024Quote: ... lysates of specific conditions were collected and the relative phosphorylation status of 37 kinase phosphorylation sites was evaluated by using a Human Phospho-kinase Array kit (ARY003C, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cells were stimulated for 18-24h in complete medium at 37˚C with addition of 50- 100U/ml human IFNγ (R&D Systems #285-IF-100).
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fibroblasts at 70-80 % confluent were serum starved overnight and treated with 50ng/ml of recombinant human PDGF-DD (R&D system, 1159-SB-025) for 24h.
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bioRxiv - Microbiology 2023Quote: ... The concentration of IL-8 released in basolateral culture media were assayed by ELISA using the human ELISA development kits (DuoSet DY208, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
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bioRxiv - Microbiology 2023Quote: ... The levels of CD83 (pg/ml) secreted in the culture medium were determined with the Human CD83 DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Developmental Biology 2023Quote: ... rmSlit-1 was used at 200 ng·mL-1 and rmEphrinA5 at 500 ng·mL-1 (R&D Systems). Spermine-NONOate was used at 50mM ...
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bioRxiv - Developmental Biology 2023Quote: ... rmSlit-1 was used at 200 ng·mL-1 and rmEphrinA5 at 500 ng·mL-1 (R&D Systems). Images were acquired in DIC every 15 sec by using a Prime95B camera (Photometrics) ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Nkx6-1 (Goat 1:200; R&D Systems AF5857). Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Nkx6-1 (Goat 1:300; R&D Systems AF5857), anti-Flag (Mouse HRP ...
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bioRxiv - Microbiology 2021Quote: ... FIZZ-1/RELM (1:1000, Ref. AF1523, R&D Systems), pCREB (1:1000 ...
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bioRxiv - Pathology 2024Quote: ... anti-mouse Mac-1 (R&D Systems, dilution 1:100) or anti-mouse CD3 (Abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... Anti-Netrin-1 antibody 1:500 (R&D Systems AF1109) in 1% BSA + 0.1% PBST was incubated at 4C overnight ...
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bioRxiv - Immunology 2023Quote: ... 1% normal rat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a humid chamber with the appropriate antibodies ...
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bioRxiv - Genomics 2023Quote: ... 1% normal goat serum and 1% BSA (R&D Systems). Samples were stained for 2h at RT in a wet chamber with the appropriate antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... LYVE-1 (1:100, AF2089, R&D Systems, Minnesota, USA) CD32B (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... intercellular adhesion molecule 1 (ICAM-1) (R&D systems, DY796), and E-selectin (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... LOX-1 (clone 214012, 1:50) from R&D Systems; Siglec-E (clone M1304A01 ...
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bioRxiv - Neuroscience 2023Quote: ... TuJ-1 monoclonal (R&D systems, Cat# MAB1195, 1:500); Alexa Fluor 568 conjugated phalloidin (Thermo Fischer Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Retinal explants were treated with 200 ng·mL-1 rmSlit-1 or 500 ng·mL-1 rmEphrinA5 (R&D Systems) diluted in warm culture medium for 20 minutes before fixation with 4% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Neuroscience 2019Quote: The secretion of cytokines in AD TREM2 neuronal cultures before and after stimulation with the Aß-S8C dimer was measured employing the Proteome Profiler Human Cytokine Array kit (R&D System, United States of America). The assay was performed following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... IP-10 and TGF-β levels were measured by enzyme-linked immunosorbent assay (ELISA) sandwich using human CXCL-10/IP-10 DuoSet ELISA (R&D Systems Inc, MN, USA) and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation ...