Labshake search
Citations for R&D Systems :
2001 - 2050 of 3961 citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 750 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
-
bioRxiv - Immunology 2020Quote: Cytokine and Chemokine secretion was assessed as previously described (van Wilgenburg et al., 2013) using the Proteome Profiler Human Cytokine Array Kit (R&D systems, #ARY005B). TNFα was quantified by ELISA (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Human Discovery Assay (R&D Systems). Custom configured targets include ...
-
bioRxiv - Bioengineering 2022Quote: ... The concentration of hCG-β in the culture medium was measured using the Human CG beta (HCG beta) Duoset ELISA (#DY9034-05, R&D Systems) according to the manufacturer protocol.
-
bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
-
bioRxiv - Immunology 2022Quote: Detection of secreted IL11 into the supernatant of HPAEpiC cultures was performed using the human IL-11 ELISA kit (R&D systems, D1100) according to manufacturers’ instructions ...
-
bioRxiv - Immunology 2022Quote: ... Culture supernatants were collected after the second treatment and TNF-α levels were quantified by ELISA using the Human TNF-alpha DuoSet ELISA (R&D systems) following manufacturer recommendations.
-
bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
-
bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems, or Shino-Test Corp., Tokyo, Japan) following the manufacturer’s protocols.
-
bioRxiv - Neuroscience 2023Quote: ... the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL recombinant human basic FGF (#233-FB, R&D Systems, Germany) and 10 µM Y-27632 (only for the first 24 h after passaging) ...
-
bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatants were used to quantify the amount of cyto/chemokines produced by the urothelial organoids before and after infection using a human premixed multi-analyte customized kit in a Luminex® bead based immunoassay (R&D systems) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: The receptor tyrosine kinase (RTK) activities in cells after drug treatment were analyzed by a Proteome Profiler Human Phospho-RTK Array Kit (R&D Systems, ARY001B) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
-
bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2023Quote: ... were assessed from the culture supernatant of the activated CD4+ T cells by Human Th9/Th17/Th22 Luminex Performance Assay 17-plex Fixed Panel (R&D systems, USA). The assay was performed according to manufacturers instructions and samples diluted 1:2 with calibrator diluent ...
-
bioRxiv - Immunology 2023Quote: ... in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA; R&D Systems) and/or 50 ng/ml IFN-γ (285-IF ...
-
bioRxiv - Bioengineering 2023Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: PB2 PE (phycoerythrin) (human, R&D Systems #FAB53291P), PB2 APC (human ...
-
bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
-
bioRxiv - Immunology 2023Quote: Levels of human IL-18 and mouse IL-1β were measured in the cell supernatants using ELISA kits from R&D Systems (DY318) and (DY401-05) ...
-
bioRxiv - Immunology 2023Quote: ITCH ubiquitination reactions were carried out using the manufacturer’s protocol for the Human ITCH/AIP4 Ubiquitin Ligase Kit (R&D Systems, K-270). 10X reaction buffer ...
-
bioRxiv - Genomics 2024Quote: ... 5 mM beta-glycerophosphate and 1% insulin-transferrin-selenous acid (osteogenic media) and stimulated them the next day with recombinant human BMP2 (300 ng/ml) (R&D Systems, MN) in serum-free osteogenic media ...
-
bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
-
bioRxiv - Immunology 2023Quote: IFN-γ secreted in the supernatant after 72h of co-culture of tumor and effector T cells was measured using the Human IFN-gamma DuoSet ELISA kit (R&D Systems, DY285B) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... and 250 μg of protein from each condition was incubated with a membrane array from the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). Membranes were processed according to the manufacturer’s instructions and imaged using an ImageQuant LAS 4000 (GE Healthcare) ...
-
bioRxiv - Systems Biology 2023Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
-
bioRxiv - Neuroscience 2020Quote: Primary antibodies for immunoprecipitation and Western blotting included anti-V5 and anti-His (R&D systems), Mouse anti-VAMP2 (SySy Synaptic Systems ...
-
bioRxiv - Immunology 2020Quote: ... beads were washed with PBS and stained with anti-PTPN22 (anti-Lyp, R&D Systems, USA) for 20min at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Anti-VE-cadherin antibody (AF1002) and anti FOXF1 antibody (AF4798) were purchased from R&D system) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Anti-Dig-POD antibody (1:100) and primary antibody (anti-Otx2, R&D Systems, Cat#: AF1979) in 100 µl of TNB were added to samples ...
-
bioRxiv - Cell Biology 2020Quote: ... for 24 hours and rested overnight in C-RPMI with 100µM ferric ammonium citrate (FeAC) and 200U/ml of human recombinant IFN-γ (R&D Systems, MN USA) unless otherwise stated.
-
bioRxiv - Developmental Biology 2020Quote: Embryonic immortalised mouse epicardium-derived cells (mEPDC) were treated with 2.0 µg/ml human recombinant transforming growth factor (TGF)-β (R&D Systems; 240-B) for 30 hrs to induce EMT ...
-
bioRxiv - Developmental Biology 2019Quote: ... Both wild type and Adgrg6 KO ATDC5 cells were treated with 100ng/ml recombinant human IL-6 protein (rIL-6) (R&D System, 206-IL) for 2 hours before protein extraction.
-
bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
-
bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
-
bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
-
bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
-
bioRxiv - Bioengineering 2021Quote: Conditioned media from each cell-line/passage (at the time of cell harvest) were collected and prepared for cytokine profiling using the Human Premixed Multi-Analyte Magnetic Luminex Assay (R&D Systems, Minnneapolis MN) (Supplemental Table 3) ...
-
bioRxiv - Biochemistry 2021Quote: Interleukin (IL)-6 in the patient plasma samples was measured by the Quantikine ELISA kit for human IL-6 (R&D Systems, Minneapolis, MN), as previously [29] ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
-
bioRxiv - Molecular Biology 2020Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Performance Assay Human High Sensitivity Cytokine Base Kit A (R&D Systems, Inc). Apical washes were diluted 1:2 ...
-
bioRxiv - Cell Biology 2020Quote: Conditioned media of papillary and reticular fibroblasts from the three donors were analysed using human angiogenesis antibody array (R&D systems, Minneapolis MN, USA). Images were acquired using Fusion Capt Advance FX7 (Vilber ...
-
bioRxiv - Cancer Biology 2022Quote: ... miniloci in an endogenous IgH and Igκ knockout background (19,20) were immunized with recombinant human LAG-3–Fc protein (hLAG-3–hFc) (R&D Systems, Minneapolis, MN, USA), consisting of the extracellular domain of LAG-3 (Leu23-Leu450 ...
-
bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
-
bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
-
bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
-
bioRxiv - Molecular Biology 2019Quote: ... administered intravenously to BALB/C mice at a dose of (0.05mg/kg of body weight and measured for serum hEpo concentration using Human Erythropoietin Quantikine IVD ELISA kit (R&D Systems cat. no. DEP00) at 6 hours post injection.