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Citations for R&D Systems :
2001 - 2050 of 2051 citations for Calcium calmodulin Dependent 3' 5' Cyclic Nucleotide Phosphodiesterase 1C PDE1C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: Macrophage supernatants were thawed on ice and assayed for the presence of 40 cytokines using Proteome Profiler Antibody Arrays (R&D Systems, Minneapolis, MN). Procedures were followed according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... anti-AMPKα2 (#AF2850, diluted at 1:1000) and anti-IL6 (#AB-206-NA, diluted at 1:500) antibodies from R&D Systems (Minneapolis, MN, USA). Secondary antibodies used for immunofluorescence labelling of fixed cells were goat anti-rabbit antibodies conjugated to Alexa Fluor 488 (#11034 ...
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bioRxiv - Developmental Biology 2023Quote: ... P0 were fixed and histologically sectioned and were stained with antibodies to detect Wnt4 (anti-Wnt4, R&D systems, cat# AF475, dilution 1:100), Wnt9b (anti-Wnt9b ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen, Germany; R&D Systems, Minneapolis, USA) and a horseradish peroxidase (HRP)-coupled secondary antibody and CheLuminate-HRP ECL solution (Applichem ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4℃ with the following primary antibodies: anti-Nestin (rabbit, 1:500, Millpore, PN-MAB5326) and anti-ki67 (mouse, 1:500, R&D system, PN-AF7649); anti-CHI3L1 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with the primary antibody for platelet-derived growth factor receptor alpha (PDGFRa) (ref. AF1062-SP, R&D systems, 1/200), OLIG2 (ref ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used: monoclonal mouse anti-human CXCR4 (clone 44717) and phycoerythrin-conjugated human CXCR4 (clone 12G5; both from R&D Systems, Minneapolis, MN); purified mouse anti-human CD3 (clone HIT3a ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Neuroscience 2020Quote: The primary antibodies used were Collagen IV (1:400 dilution; Cosmo Bio, Catalog Number LSL-LB1403) and CD13 (1:100 dilution; R&D Systems, Catalog Number AF2335). Mice were sacrificed by decapitation ...
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bioRxiv - Genomics 2020Quote: ... LDLR staining was performed for 30 min on ice with a 1:50 dilution of AlexaFluor488-conjugated LDLR antibody (R&D Systems, Minneapolois MN, FAB2148G) into PBS supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated with the primary antibodies (1:100 dilution; CD68, Bio-Rad, Cat# MCA1957; iNOS, Abcam, Cat# ab15323; CD206, R&D Systems, Cat# AF2535) in TBST containing 2% BSA for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Caco2 cells were pre-treated for 30 min at 37°C with goat antibody directed against the human ACE2 or DDP4 ectodomain (R&D Systems, Wiesbaden-Nordenstadt, Germany). Then ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using the following primary and secondary antibodies: anti-IL-1β (R&D systems, AF-201-NA), anti-GFP (Clontech ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and resuspended in FACS buffer containing Human Fibroblast Activation Protein alpha PE-conjugated Antibody (FAP-PE) (R&D systems FAB3715P, Clone # 427819) on ice in darkness for 30min ...
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bioRxiv - Developmental Biology 2022Quote: ... In-house purified rhFGF10 was confirmed by western blot analysis using anti-FGF10 antibody and compared to commercially purchased rhFGF10 (R&D Systems, Cat#345-FG) to test/validate activity based on the efficiency to phosphorylate ERK1/2 in an A549 alveolar epithelial cell line (ATCC ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Developmental Biology 2019Quote: ... blocked (5 % BSA and 0.1 % Tween 20 in 1X TBS) for at least 1 hour at room temperature and stained with primary antibodies (Phospho-STAT3 Y705 and STAT3, R&D Systems 9131S and 9132S) diluted in blocking solution overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
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bioRxiv - Developmental Biology 2019Quote: Western blotting was performed as previously described (Chang et al., 2016, Mariani et al., 2016, Bay et al., 2018) with the following antibodies: Gli2 (R&D Systems, AF3635, 1:500), Gli3 (R&D Systems ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... transferred to a fresh reaction tube and subsequently washed three times with 500 μl PBS-T before addition of primary antibody (R&D systems, Minneapolis, MN, USA) 1:5000 in 200 μl sample buffer ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
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bioRxiv - Pathology 2023Quote: ... CXCL2, CXCL1, TNF, MMP8, S100A8, IFN-γ, IL12p40, IL-12p70, IL-10, and VEGF using antibody pairs and standards from R&D Systems (Minneapolis, MN), Invitrogen (Carlsbad ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... EV samples at a concentration of 1 × 1010 particles/mL were incubated with AF647-labelled anti-TSPAN2 antibody (R&D systems, FAB7876R-100UG, 0.5 µL) or a mixture of APC-labelled anti-CD9 (Miltenyi Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transferred to wet ice and the remaining surface receptor was labeled with 1:100 anti-rabbit antibody conjugated to PE (F0110, R&D Systems, Minneapolis, MN, USA) in FACS buffer for 30 min on ice and protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies (1:4000: Cell Signaling Technology; 1:2,000: R&D Systems, Bio-Techne; Santa Cruz Biotechnology) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 2.5% BSA/0.1% Triton-X for 1 hour and subsequently incubated with an antibody cocktail consisting of goat anti-mouse PDGFRα (1:100) (catalog #: AF1062; R&D Systems; Minneapolis, MN, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: Paraffin-embedded specimens of brown adipose tissue (BAT) were cut and immunostained with antibody to uncoupling protein-1 UCP-1 (R&D Systems, cat. # MAB6158, Minneapolis, MN) at a concentration of 10 µg/mL by personnel of the Experimental Pathology Core of NYU School of Medicine ...
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bioRxiv - Molecular Biology 2023Quote: ... The sections were blocked with 5% skimmed milk in TBS-T at room temperature for 60 min and then incubated with rabbit anti-mMCP-6 antibody (R&D Systems, MN, USA, 1:500) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were isolated and stained with anti- human CD3 and CD8a antibodies (surface staining) followed by intracellular staining with anti- human IFNγ (R&D Systems, Cat. No. IC285F-100) antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... Following primary antibodies (αTFAP2A, Abcam ab108311; αTFAP2B, Abcam ab221094; αLEF1, Cell Signaling, 2230; αSOX2, R&D Systems, AF2018; αPITX1, Abcam ab70273; and αPITX2, R&D Systems, AF7388) were diluted in TBS (0.5% Tween in phosphate-buffered saline ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: Immunohistochemical staining for ADIPOR1 and MFRP was conducted on tissues that were fixed in formalin and embedded in paraffin. The antibodies were rabbit anti-Adiponectin Receptor 1 (IBL, Catalog No. 18993, Japan) and goat anti-mouse MFRP (R&D Systems, Catalog No. BAF3445, Minnesota) respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution). After incubation with primary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Cancer Biology 2020Quote: ... was confirmed by immunohistochemistry on FFPE tissues after antigen retrieval (pH 9) and endogenous peroxidase inhibition followed by overnight incubation with mouse antibody to SBase (anti-SBase; R&D Systems; pH 9; 1:200 dilution), followed by incubation with primary antibody ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies (FUS 1:100 Santa Cruz Biotech sc-47711; ISL1 1:500 Abcam ab109517; OLIG2 1:100 R&D Systems AF2418; NFM 1:1000 Merck MAB1621) were added in 1% BSA and incubated at 4degC overnight ...