Labshake search
Citations for R&D Systems :
1951 - 2000 of 4619 citations for Human IgM Anti Flavivirus Envelope Protein Antibody 4G2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... every third sagittal section of tissue containing the left fastigial nucleus was blocked with 5% bovine serum albumin for one hour at room temperature prior to incubation in primary antibody goat anti-osteopontin overnight at 4°C (1:300, R&D Systems AF808; RRID:AB_2194992). Tissue was then rinsed and incubated in donkey anti-goat Alexa Fluor 594 for 2 hours at room temperature (1:500 ...
-
bioRxiv - Immunology 2019Quote: ... sections were incubated overnight at 4°C with an antibody mix containing rat anti-mouse IL-18 (5 μg/ml; D047-3; R&D Systems, MN, USA) and rabbit anti-EpCAM (Clone ab71916 ...
-
bioRxiv - Neuroscience 2022Quote: ... Non-specific blocking for 30 minutes in 8% horse serum in KPBS was completed before incubation in primary antibody (anti-HPLN1 1:150, R&D Systems 2608-HP) for 48 hours at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) with 0.1% Tween 20) for 30 minutes and probed with primary antibodies: polyclonal goat anti-C3d (R&D systems, AF2655, 1:1000), polyclonal sheep anti-Sez6L2 (R&D Systems ...
-
bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
-
bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... and stained for 30 minutes on ice with a 1:50 dilution of AlexaFluor-647 conjugated anti-LTB4R antibody (R&D Systems; catalog #: FAB099R) in FACS buffer (0.5% bovine serum albumin and 0.05% sodium azide in PBS) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred to a membrane using the iBlot2 system and incubated overnight at 4°C with primary antibody (anti-Gli3, 1:500, AF3690, R&D Systems, Minneapolis, MN). Membranes were then incubated in secondary antibody (anti-goat Alexafluor 680 ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by incubation with anti-CD31 primary antibody (1:200, #550274, BD Biosciences, San Jose, CA, USA; #BBA7, R&D systems, Minneapolis, MN, USA) and Alexa Fluor 594-conjugated secondary antibody.
-
bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen, Germany; R&D Systems, Minneapolis, USA) and a horseradish peroxidase (HRP)-coupled secondary antibody and CheLuminate-HRP ECL solution (Applichem ...
-
bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... P0 were fixed and histologically sectioned and were stained with antibodies to detect Wnt4 (anti-Wnt4, R&D systems, cat# AF475, dilution 1:100), Wnt9b (anti-Wnt9b ...
-
bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
-
bioRxiv - Immunology 2024Quote: ... anti-AMPKα2 (#AF2850, diluted at 1:1000) and anti-IL6 (#AB-206-NA, diluted at 1:500) antibodies from R&D Systems (Minneapolis, MN, USA). Secondary antibodies used for immunofluorescence labelling of fixed cells were goat anti-rabbit antibodies conjugated to Alexa Fluor 488 (#11034 ...
-
bioRxiv - Immunology 2023Quote: ... Plasma C-reactive protein levels were assessed by a Mouse C-Reactive Protein/CRP Quantikine ELISA Kit (R&D Systems, Inc., MN, USA) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2021Quote: ... The following antibodies were used as detector antibodies for tetraspanins: ab58989 (R&D Systems), 556019 (BD ...
-
bioRxiv - Genomics 2020Quote: Plasma was used to assess circulating monocyte chemoattractant protein (MCP)-1 and C-reactive protein (CRP) via ELISA following manufacturer’s instructions (R&D Systems, Minneapolis, MN, USA) (1).
-
bioRxiv - Biophysics 2021Quote: ... and S-protein (10549-CV-100, R&D systems, UK) at concentrations specified in the main text ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse EGF and mouse FGF basic protein (R&D Systems) and 50% of Matrigel (Corning) ...
-
bioRxiv - Developmental Biology 2021Quote: ... mouse EGF and mouse FGF basic protein (R&D Systems). Inhibitors (Table 1 ...
-
bioRxiv - Microbiology 2019Quote: ... or FGF-2 protein levels using ELISA (R&D systems). The LDH release was calculated as described previously (83).
-
bioRxiv - Cell Biology 2021Quote: ... rh-HyperIL6 (IL6R:IL6 fusion protein, 8954-SR, R&D systems), rm-HyperIL6 (IL6R:IL6 fusion protein ...
-
bioRxiv - Cell Biology 2021Quote: ... rm-HyperIL6 (IL6R:IL6 fusion protein, 9038-SR, R&D systems), human soluble gp130 Fc (671-GP-100 ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 nM or 10 nM rhGALC protein (R&D Systems) was added to the microglia medium at the time of seeding into the assay plate.
-
bioRxiv - Physiology 2021Quote: ... recombinant mouse BMP4 Protein (R&D Systems, 5020-BP-010) [diluted in 0.1% BSA ...
-
bioRxiv - Microbiology 2021Quote: Soluble full-length NgR1 protein (R&D Systems, AF1208-NG) was immobilized on model surfaces using Protein A chemistry as described [106] ...
-
bioRxiv - Microbiology 2022Quote: ... caninum NIF protein (R&D Systems; 0.5 µg ml−1) for 20 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... recombinant mouse PD-1-Fc chimeric protein (R&D Systems) or human IgG1 control (R&D Systems) ...
-
bioRxiv - Physiology 2022Quote: ... Purified SEMA3F protein (100ng/kg, 3237-S3, R&D Systems) was administered via i.p injections ...
-
bioRxiv - Microbiology 2023Quote: ... recombinant mouse Gas6 protein (R&D Systems, cat# 986-GS) (used in administration experiments in mice) ...
-
bioRxiv - Genetics 2023Quote: Recombinant Mouse IFNγ Protein (R&D Systems, 485-MI-100) was added to the NPC medium at a concentration of 10 ng/ml from day 0-5 ...
-
bioRxiv - Genetics 2023Quote: Recombinant Mouse IFNγ Protein (R&D Systems, 485-MI-100) was added to the iPSC medium at a concentration of 10 ng/ml from day 0-5 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and dickkopf-related protein 1 (R&D Systems; Catalog #DY1756). All ELISA assays were run in duplicate according to manufacturer protocols ...
-
bioRxiv - Cell Biology 2022Quote: ... or ECM proteins fibronectin (R&D systems #3420-001-01) and collagen-I (Chrono-Log #385 ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with CD22-Fc protein (R&D Systems) in PBS for 30 min on ice and washed with PBS twice to remove the unbonded protein ...
-
bioRxiv - Immunology 2023Quote: ... Then biotinylated Spike proteins (WA-2, 2P, R&D systems) were subsequently captured to a level of 3-500RU by the neutravidin ...
-
bioRxiv - Immunology 2023Quote: ... recombinant mouse Fc-chimera proteins CD6 (rCD6, R&D Systems) and/or ICAM-1 (rICAM1 ...
-
bioRxiv - Developmental Biology 2023Quote: ... BMP4 protein was purchased from R&D Systems (5020-BP). LDN193189 was provided by Dr ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using the following primary and secondary antibodies: anti-IL-1β (R&D systems, AF-201-NA), anti-GFP (Clontech ...
-
bioRxiv - Developmental Biology 2022Quote: ... In-house purified rhFGF10 was confirmed by western blot analysis using anti-FGF10 antibody and compared to commercially purchased rhFGF10 (R&D Systems, Cat#345-FG) to test/validate activity based on the efficiency to phosphorylate ERK1/2 in an A549 alveolar epithelial cell line (ATCC ...
-
bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
-
bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
-
bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
-
bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
-
bioRxiv - Synthetic Biology 2023Quote: ... EV samples at a concentration of 1 × 1010 particles/mL were incubated with AF647-labelled anti-TSPAN2 antibody (R&D systems, FAB7876R-100UG, 0.5 µL) or a mixture of APC-labelled anti-CD9 (Miltenyi Biotech ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were transferred to wet ice and the remaining surface receptor was labeled with 1:100 anti-rabbit antibody conjugated to PE (F0110, R&D Systems, Minneapolis, MN, USA) in FACS buffer for 30 min on ice and protected from light ...