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Citations for R&D Systems :
151 - 200 of 1197 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... the media was incubated (72 h) with media containing different concentration of recombinant human PTN (rhPTN, R&D systems 252 - PL) or the drugs alectinib (Toronto Research Chemicals ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were seeded on Matrigel coated culture plates in mTeSR supplemented with 10µM Y-27632 dihydrochloride (R&D Systems, 1254/10) at a density of 56,000/cm2 for routine maintenance of iPSC lines ...
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bioRxiv - Cancer Biology 2020Quote: ... Macrophage differentiation from bone marrow cells was obtained by culture in standard growth medium supplemented with m-CSF (R&D Systems) at 30 ng/ml for 7 days.
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bioRxiv - Molecular Biology 2020Quote: Cytokine levels in cell culture supernatants samples were determined using the Proteome Profiler Mouse Cytokine Array Panel A (R&D Systems) following the manufacturer recommendations.
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad) ...
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bioRxiv - Neuroscience 2019Quote: ... Cells incubated at 37°C/5%CO2 for approximately 1hr prior to flooding the dishes with F12 complete media alone or complete F12 media containing 100ng/mL GDNF (R&D Systems). Cells incubated overnight at 37°C/5%CO2 prior to being rinsed in PB and fixed in 3% paraformaldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... On the following day the media was replaced with quiescence media in which EGF was exchanged for BMP4 (50 ng/ml, R&D Systems). The cells were cultured for a total of 3 days with fresh media exchanged every 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... media was replaced with fresh media containing MCSF and receptor activator of nuclear κB (RANKL) (25 ng/mL each, R&D Systems), and media refreshed every 2-3 days thereafter until a total of seven days stimulation with RANKL ...
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Osteoclast differentiation media consisted of standard OCY454 media supplemented with 25ng/ml M-CSF (R&D Systems Catelog # 416-TEC; Lot # ME5021121) and 5 ng/ml RANKL (R&D Systems Catalog #462-TEC-010 ...
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bioRxiv - Immunology 2019Quote: ... Autologous NK cells were isolated by negative selection using a human NK Cell Isolation Kit (Miltenyi) and cultured in complete RPMI-1640 media with 300 IU/mL of IL-2 (R&D Systems) for 22 hours.
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: Culture media of cells infected with virus were collected at the indicated times and used for ELISA quantification of IFNβ (R&D Systems) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells from each well were harvested and resuspended in 100 µL of cell resuspension solution provided with human methylcellulose enriched media (R&D systems). Each cell suspension was added to 2.5 mL methylcellulose enriched media ...
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bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...
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bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
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bioRxiv - Cell Biology 2022Quote: ... media with 10% fetal bovine serum (R&D systems), 1% Penicillin-Streptomycin (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 1.5nM BMP4 (R&D Systems), and half media changes were carried out every other day until Day 16 to dilute the rhBMP4 ...
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bioRxiv - Cell Biology 2022Quote: ... media with 10% fetal bovine serum (R&D systems), 1% Penicillin-Streptomycin (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... 2% Noggin/R-Spondin–conditioned media (R&D Systems), 100 ng/mL recombinant human Wnt3A (R&D Systems) ...
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bioRxiv - Cell Biology 2024Quote: ... R-spondin1 conditioned media (R&D Systems, Minneapolis, MN), and CHIR99021 (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of ANGPT2 secreted from HUVEC into the cell culture supernatant was quantified using Human Angiopoietin 2 DuoSet ELISA kits (R&D systems), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatant from treated cells were collected and applied to the IP-10 antibody-coated plate for ELISA analysis (R&D Systems) following the manufacture’s instruction.
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bioRxiv - Immunology 2020Quote: ... cell culture supernatants were collected and IL-8 secretion was determined using IL-8 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Immunology 2023Quote: IFNβ and CXCL10 release in the culture medium of EndoC-βH1 cells was measured using enzyme-linked immunosorbent assays (Quantikine ELISA kit, R&D Systems). Each condition was measured from the supernatant of 35.000 cells cultured in 200 µL medium and culture conditions are indicated in the legend of the figures.
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bioRxiv - Immunology 2023Quote: Protein levels of the cytokines CXCL-10 and IL-6 in the cell culture supernatants were determined using the following ELISA kits: CXCL-10 and IL-6 (R&D System) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Stimulation was carried out in 96 well plates pre-coated with DLL4 and VCAM1 as described above in 100µL per well late T cell differentiation media supplemented with 20ng/mL IL-21 (R&D Systems, 8879-IL) and 1.25% ImmunoCult Human CD3/CD28/CD2 T cell activator (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently cell clusters were differentiated into β-like cells by exposure to the appropriate media as previously published.29 All recombinant proteins were purchased from R&D System unless otherwise stated (see Supplementary Table 3).
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bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cultrex 3D Culture Matrix Laminin I (R&D systems), blebbistatin (Sigma) ...
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bioRxiv - Genetics 2023Quote: ... neuronal differentiation was started (Day 0) by switching feeding media to N2B27 neural induction media supplemented with 500 ng/μL noggin (R&D Systems, #3344-NG). N2B27 neural induction media consisted of Neurobasal™ Medium (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... the media were further supplemented with RSPO1 (R&D Systems), LDN193189 (Stemgent ...
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bioRxiv - Cell Biology 2024Quote: ... and R-spondin1 conditioned media (R&D Systems, Minneapolis, MN). The medium was changed in 2 days ...
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bioRxiv - Pathology 2020Quote: Osteoprotegerin was measured in culture supernatants of cells and slices using a murine OPG DuoSet® ELISA kit (R&D Systems, Minneapolis, US) according to the instructions provided by the manufacturer.
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Purification of immunostimulatory glucose homopolymer from broccoli (Brassica oleracea var. italica)bioRxiv - Biochemistry 2021Quote: ... We collected the cell culture supernatant and measured the TNF-α concentration using Mouse TNF-α Quantikine ELISA Kit MTA00B (R&D SYSTEMS).
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bioRxiv - Developmental Biology 2021Quote: ... Cells were removed from adherent cultures using AssayComplete cell dissociation reagent (Eurofins #92-0009) and incubated with a polyclonal anti-ALK2 antibody (R&D Systems #AF637) or a polyclonal anti-ALK3 antibody (Sino Biological #50078-RP02) ...
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bioRxiv - Cell Biology 2022Quote: Expression levels of TGFA and VEGF-A in cell culture supernatants were measured using Human Quantikine ELISAs (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The two cytokines IFNγ and TNFα were detected from cell culture supernatants of the degranulation assays utilizing the respective R&D DuoSet (R&D Systems, USA) ELISAs per manufacturers’ recommendation.
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bioRxiv - Cell Biology 2024Quote: Secreted proteins were measured in cell culture supernatants from treated iBMDMs using ELISAs for IL-1β and CCL5 (Quantikine®, R&D systems).
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Immunology 2024Quote: IFN-γ protein levels were measured from cryopreserved neuron-CD8+ T cell co-culture supernatant using the Mouse IFN-γ DuoSet ELISA kit from R&D Systems (R&D Systems ...
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bioRxiv - Immunology 2023Quote: IFN-γ secreted in the supernatant after 72h of co-culture of tumor and effector T cells was measured using the Human IFN-gamma DuoSet ELISA kit (R&D Systems, DY285B) following the manufacturer’s instructions ...