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Citations for R&D Systems :
151 - 200 of 2499 citations for Collagen 17 alpha 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Levels of lymphocyte-derived cytokines (IFNγ, IL-17) were determined after 7 days (PeliKine Compact or Duoset ELISA, R&D Systems). Baseline inflammatory plasma markers from both cohorts ...
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bioRxiv - Immunology 2021Quote: ... Protein lysates from colon tissue (normalized to 3.5 mg/mL) were assayed in duplicate using the Mouse IL-17 DuoSet ELISA (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with RGDS peptide (10 μg/mL, R&D systems, MN, USA) (20 ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... incubated with the fluorogenic peptide substrate Z-FR-AMC (150 μM; R&D Systems) and measured at a fluorometer following a 0 to 30 min incubation (ƛEx = 390 nm ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Neuroscience 2019Quote: ... for 24h or with 10 ng/mL tumor necrosis factor alpha and 10ng/ml IL1-ß(R&D Systems; 401-ML-005) for 24h ...
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bioRxiv - Immunology 2019Quote: ... normal T cell expressed and secreted (RANTES) and tumor necrosis factor alpha (TNF-α) by ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Immunology 2022Quote: ... Urine albumin and creatinine were measured using ELISA kits (Alpha Diagnostic International, San Antonio, USA, and R&D systems, Minneapolis, USA, respectively). Urine samples were diluted to fit within the standards range of each kit ...
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bioRxiv - Neuroscience 2022Quote: ... 506-RL-010/CF) and carrier free Recombinant Mouse TNF-alpha (aa 80-235) Protein (410-MT-025/CF) purchased from R&D Systems.
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Biochemistry 2023Quote: ... were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml, R&D systems 3440-100-01) at 37°C in 5% CO2 in high glucose-containing DMEM (Corning 10-013-CM ...
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bioRxiv - Neuroscience 2021Quote: Protein was harvested from WT and p.Glu355Lys iPSC and NCC lysates (d19 of differentiation) using the manufacturer’s Receptor Tyrosine Kinase (RTK) array protocol in Lysis buffer 17 (R&D Systems, # ARY001B). RTK arrays were blocked for 1 hour at room temperature in Array Buffer 1 (manufacturer’s protocol) ...
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bioRxiv - Bioengineering 2021Quote: ... We performed the blocking experiment using an ACE2 polyclonal goat antibody (Cat # AF933; R&D systems) and CD147 (BSG ...
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bioRxiv - Cancer Biology 2020Quote: IL6 and soluble IL6RA concentrations were assessed in 100 µl cultured media obtained from HMECs or MCF 10A cells propagated under anchorage dependent or anchorage independent conditions with the Human IL-6 DuoSet or Human sIL-6R alpha DuoSet ELISA kit (R&D Systems, Germany) following the manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... Culture supernatants were collected after the second treatment and TNF-α levels were quantified by ELISA using the Human TNF-alpha DuoSet ELISA (R&D systems) following manufacturer recommendations.
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bioRxiv - Cell Biology 2019Quote: ... SKBr3 cells co-transfected with EGFP-α-tubulin and the indicated siRNA or cDNA were grown on collagen-coated glass coverslips for 48 h and observed 10 min after the addition of 5 nM HRGβ1 (R&D Systems) using the 63X objective (plan Apochromat NA 1.4 ...
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bioRxiv - Genomics 2023Quote: ... were assessed from the culture supernatant of the activated CD4+ T cells by Human Th9/Th17/Th22 Luminex Performance Assay 17-plex Fixed Panel (R&D systems, USA). The assay was performed according to manufacturers instructions and samples diluted 1:2 with calibrator diluent ...
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bioRxiv - Physiology 2023Quote: ... Then the tumor necrosis factor alpha (TNFA) and interleukin 10 (IL10) protein levels in the tissue were measured by enzyme linked immunosorbent assay (ELISA) (R&D Systems, Abingdon, UK).
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bioRxiv - Microbiology 2021Quote: ... surface blocking was conducted by incubated surfaces with anti-NgR1 antibody (R&D Systems; 100 µg/mL). Measurements were taken at a retraction velocity of 1 µm/s ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2021Quote: ... one ml solution of 250 ng/ml type I collagen with or without 250 ng/ml POSTN (R&D Systems, Minneapolis, MN) was added to each well followed by one-hour incubation at room temperature ...
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bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and resuspended in FACS buffer containing Human Fibroblast Activation Protein alpha PE-conjugated Antibody (FAP-PE) (R&D systems FAB3715P, Clone # 427819) on ice in darkness for 30min ...
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bioRxiv - Bioengineering 2023Quote: RFP-MDA-MD-231 and GFP-HUVEC at 3 million/mL and 8 million/mL in precursor solution were photopatterned and perfused using the peristaltic pump previously mentioned at 0.2 rpm/mL for five days before tumor necrosis factor-alpha (TNF-α) (R&D Systems, Minneapolis, MN, USA) and Vascular endothelial growth factor (VEGF ...
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bioRxiv - Cancer Biology 2019Quote: ... human IFN-β blocking antibody (Clone AF814; R&D) and its corresponding isotype (Goat IgG, R&D Systems) were used at a concentration of 0.2 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... and in the indicated experiments with 20 μg/mL of blocking anti-NKG2D antibody (R&D systems MAB1547). Cells were then washed ...
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bioRxiv - Immunology 2019Quote: We measured the concentrations of IL-1β and tumor necrosis factor alpha (TNF-α) in culture supernatants by using a commercial ELISA kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Genetics 2020Quote: MEFs cultured in collagen-coated six-well plates were transiently transfected with indicated expression vectors and stimulated overnight with100 nM PMA (R&D Systems, Minneapolis, USA). Cell culture supernatant was centrifuged at 2500xg at 4°C for 5 min and 100 uls of supernatant was used to measure AREG protein levels by a Mouse Amphiregulin DuoSet ELISA Developmental Kit (#DY989 ...
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bioRxiv - Immunology 2023Quote: ... CD4 and CD8A hispid cotton rat (Sigmodon hispidus) sequences were taken from commercially available constructs (Cotton Rat CD4 VersaClone cDNA, cat# RDC1063, R&D Systems; Cotton Rat CD8 alpha (AAL55392) VersaClone cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
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bioRxiv - Cancer Biology 2020Quote: C57BL6/J male mice (#000664) received an intraperitoneal injection of 45μg anti-CCL20 blocking antibody (R&D Systems clone 114908) or rat IgG isotype control antibody (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... pH6.0 at 95°C followed by 1h blocking and incubation with pre-optimized primary anti-UCHL1 (MAB6007, R&D Systems) or anti-p53 (Dako ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissue was permeabilized with 0.3% Triton X and quenching and blocking was performed using a cell and tissue staining kit (R&D Systems) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... the cultures were supplemented with 20 μg/ml of a pan-vertebrate anti–TGF-β blocking antibody 1D11 (R&D Systems), alongside stimuli addition ...
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bioRxiv - Immunology 2022Quote: ... BAL cells were treated for 2h before infection with an ACE2 blocking antibody at 10 μg/ml (AF933, R&D systems). SARS-CoV-2 was added to the cells at MOI 0.1 or 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 M/mL PyMT tumor cells were suspended in collagen +/-L-ribose supplemented with basement membrane extract [20 % v/v final (R&D Systems, Cultrex BME, 3532-005-02) and DMEM [1x final] and the pH was adjusted to ∼7.3 ...
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bioRxiv - Immunology 2020Quote: Titration of blocking antibodies was performed by using static adhesion assays in which activated CD4+ T cells were allowed to adhere to either recombinant human ICAM/Fc or VCAM/Fc chimeras in the presence of blocking antibodies specific for integrin α4 (clone 2B4, R&D Systems) or integrin αL (clone TS1/22 ...
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bioRxiv - Immunology 2023Quote: ... 3129), for CCR5, with Maraviroc (10 μM, 3756) and for blocking CXCR3 with AMG487 (10 μM, 4487) all from R&D Systems, at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... separated and blotted onto 0.2 μm nitrocellulose as above and incubated overnight at 4°C in blocking buffer supplemented with 2 μg/ml polyclonal sheep IgG anti-LL-37 (R&D Systems). Membranes were washed in wash buffer (PBS with 0.05% Tween ...
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bioRxiv - Microbiology 2024Quote: ... the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 µg/ml, R&D Systems, MAB17781) for 1h at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Verification of TIMP activity in vitro using activated MMP-2 and the fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 was performed as per the protocol provided by R&D Systems.
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bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Immunology 2021Quote: ... fifty microliters of stimulant pepmixes (JPT peptides) resuspended in RPMI+10%FBS (R10) with recombinant mouse IL-2 (rmIL-2) (R&D Systems) were added to each well ...
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bioRxiv - Microbiology 2023Quote: PLY-treated H292 monolayers or PMNs in suspension were treated with 1U PLY and incubated for 30 min at 37 °C with 10 μM fluorogenic peptide MMP substrate (Mca-PLAQAV-Dpa-RSSSR-NH2, R&D Systems) diluted in 25 mM Tris buffer ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated in 0.5% serum-containing media before treatment with 200 ng/mL recombinant Shh N-terminal peptide (R&D Systems, 1314-SH) diluted in dimethyl formamide (DMF).