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Citations for R&D Systems :
151 - 200 of 2694 citations for 8 N N Diethylamino octan 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... For 8 oxo-dG we performed ICC on PFA fixed cells with a specific antibody (R&D System) to quantify DNA oxidation ...
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bioRxiv - Developmental Biology 2024Quote: ... 6 and 8 the culture medium was replaced with fresh N2B27 + 50 ng/μL BMP4 (R&D Systems) and analyses performed on day 9.
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bioRxiv - Immunology 2023Quote: ... IL-8 and CXCL1 cytokines within were quantified by ELISA according to manufacturer instructions (Duoset, R&D Systems). For cytokine antibody array ...
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bioRxiv - Immunology 2022Quote: Human IL-8 was measured from cell culture supernatants using commercially available specific paired ELISA kits (R&D Systems). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Plasma IL-8 and TNF-α levels were measured using mouse ELISA kits (R&D System, Minneapolis, MN, USA).
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bioRxiv - Neuroscience 2021Quote: ... ~70,000 cells/well were seeded in a 8-chamber slide coated with either 10μg/mL rNPNT (R&D system), 2μg/mL BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: Caspase-8 inhibitor Z-IETD-FMK and Caspase-9 inhibitor Z-LEHD-FMK were obtained from R&D Systems and were dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Genetics 2023Quote: ... or vehicle for 24 hours followed by 8 hours of recombinant TNFα (2ng/ml, R&D Systems, Minneapolis MN). Similar experiments were conducted with pre-treatment of cells for 2 hours with Pyrrolidine Dithiocarbamate (PDTC ...
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bioRxiv - Immunology 2019Quote: ... IL-8 and Cxcl15 were analyzed by ELISA kits following the manufacturer’s instructions (BD Bioscience and R&D Systems, respectively).
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bioRxiv - Neuroscience 2023Quote: ... IL-6 and IL-8 levels in conditioned media were quantified using Quantikine immunoassays (HS600C and D8000C, R&D systems). GFAP was measured in CM using Human GFAP DuoSet ELISA (R&Dsystems ...
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bioRxiv - Genomics 2019Quote: 8-week iEPs were stained with mouse E-cadherin-APC antibody (10 μL per one million cells, R&D Systems, FAB7481A) and sorted on a modified Beckman Coulter MoFlo into EcadHigh and EcadLow populations ...
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bioRxiv - Microbiology 2021Quote: ... IFNβ and IFNλ protein levels from HCT-8 cultures were measured by Human IFN-beta DuoSet ELISA (DY814, R&D Systems) and Human IL29/IL28B (IFN-lambda 1/3 ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... Human IL-8/CXCL8 Quantikine ELISA kit and non-immune human IgG control were purchased from R&D Systems (Minneapolis, MN). Phorbol myristate acetate (PMA) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 12-well plates and treated for 8 hours in biological quadruplicates with IFNα (0.5 ng/ml, R&D Systems), IFNβ (0.5 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC).
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bioRxiv - Microbiology 2019Quote: ... Mouse and human CXCL1 and CXCL2 as well as human IL-8 (CXCL8) were obtained from R&D Systems (Minneapolis, MN) and multiplex CXCL1 ...
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bioRxiv - Bioengineering 2020Quote: We used the method of Cell-based ELISA with using a Human Cleaved Caspase-8 (Asp391) Immunoassay kit (R&D systems).
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bioRxiv - Microbiology 2020Quote: ... Released IL-8 was determined in supernatants collected at 24 hrs using an ELISA kit (Biosource Europe and R&D Systems), according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... CRP and VDBP were conducted using Luminex® Magnetic Screening Assays (Catalogue number LXSAHM-8, R&D System, Minneapolis, MN, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The cell-free supernatants derived from CD10neg/CD10pos neutrophils were added to CD4+ T-cells cultured in 96-well plates (8×104 cells/well) in the presence of neutralizing anti-IL-12 antibody (4μg/mL; MAB219, R&D Systems) or isotype control (4μg/mL ...
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bioRxiv - Immunology 2021Quote: Mice aged 8-10 weeks were given 0.75 μg/g body weight αCCL11 and αCCL24 (R&D Systems; MAB420 and MAB528) or an equivalent amount of rat IgG (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... 5% CO2 for 8 days with 100 ng/ml macrophage colony-stimulating factor (M-CSF; R&D systems, cat.no 216-MC). Fully differentiated macrophages were harvested and seeded at the required density for the subsequent experiments.
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bioRxiv - Microbiology 2024Quote: Cytokine secretion in CF BALF was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453 ...
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bioRxiv - Cell Biology 2020Quote: ... and then were seeded into upper chamber of transwell inserts (8 μM pore size, Costar) with or without coated Matrigel (R&D System). The lower chamber of the transwell was filled with 750 μl medium supplemented with 10% FBS which function as a chemoattractant ...
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bioRxiv - Cell Biology 2020Quote: ... An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Microbiology 2021Quote: Protein expression was analyzed by polyacrylamide gel electrophoresis on 8%-16% precast gradient gels (Thermo) and Western blotting using antibodies to ACE2 (AF933, R&D Systems), c-Myc-epitope (clone 9E10 ...
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bioRxiv - Cancer Biology 2019Quote: ... and the corresponding cryostat tissue sections (8-10μm thickness) were stained using the following primary antibodies: goat anti-CD31 (R&D System; AF3628), rabbit anti-caspase-3 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... It was counted as Day 0 when medium was changed to SF medium with 8 ng/mL recombinant Activin A (R&D Systems), 5 ng/mL recombinant human VEGF 165 Protein (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Molecular Biology 2023Quote: ... and IL11 in equal volumes of cell culture media collected from experiments with primary human cells were quantified using Human IL-8/CXCL8 Quantikine ELISA Kit (D8000C, R&D Systems), Human IL-6 Quantikine ELISA Kit (D6050 ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed EVs were concentrated with 3 kDa MWCO centrifugal filters and characterized via Luminex Multiplex Assay for TNF-α and IL-8 according to the manufacturer’s instructions (R&D Systems). Results were normalized by total protein content ...
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bioRxiv - Developmental Biology 2022Quote: Cells were dissociated with TrypLE on day 6 and 8 of CMEC and PMEC protocol and stained with VE-Cadherin PE-conjugated Antibody (R&D Systems). Then VEC+ cells were sorted using FACSAria III ...
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bioRxiv - Molecular Biology 2023Quote: ... and apurinic or apyrimidinic (AP or abasic) sites in the samples were performed using HT 8-oxo-dG ELISA Kit (R&D Systems) and AP Sites Quantitation Kit (Cell Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... and MCP-1 were determined in efflux collected on day 8 using a custom version of the Human XL Cytokine Performance Panel (R&D systems). SHBG levels were quantified using the Human XL Cytokine Discovery Panel (R&D systems) ...
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bioRxiv - Microbiology 2019Quote: ... IL-8 production was also determined by analysis of basolateral supernatants from VDC’s using a human IL-8 ELISA kit (R&D systems, Minneapolis, USA) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... 50μl of mitochondria (1μg/μl protein) were treated with Caspase-8-cleaved tBid from 5-500nM (882-B8-050, R&D Systems, Minneapolis, MN), Bim-BH3 from 10-10,000nM (Ac-DMRPEIWIAQELRRIGDEFNAYYARR-amide ...
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bioRxiv - Microbiology 2021Quote: ... The levels of IL-1β and IL-8 in the conditioned medium were measured by ELISA using the human IL-1β DuoSet (R&D Systems; # DY20105) and the human IL-8 set (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... IL-8 and TNF-α were quantified in the supernatants from uninfected and SARS-CoV-2-infected hiPSC-CMs by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
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bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems, or Shino-Test Corp., Tokyo, Japan) following the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... mice (6-8 weeks old) were injected with 1x106 cells suspended in PBS and Cultrex™ basement membrane extracts (BME) (Bio-techne/R&D Systems) into the right flank ...
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bioRxiv - Microbiology 2021Quote: Virus-inactivated serum samples or lung supernatants from SARS-CoV-2 VoC infected mice were added to a custom 8-plex Mouse Magnetic Luminex Assay (R&D Systems LXSAMSM-08) including IL-6 ...