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Citations for R&D Systems :
151 - 200 of 3762 citations for 5 1 3 benzothiazol 2 ylamino pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Total TGF-β levels were determined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems).
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... explants were incubated overnight in PBS 3% BSA with primary antibody: anti-Robo1 (R&D Systems, 1:400) or anti-Robo2 (gift from Dr Alain Chédotal 53 ...
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bioRxiv - Systems Biology 2020Quote: ... or mouse anti-TNFα Receptors 1 and 2 (10 µg/mL, R&D Systems) (see Figure 1-Figure Supplement 4B ...
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bioRxiv - Pathology 2021Quote: ... 1% Antibiotic-Antimycotic and 20ng/ml recombinant fibroblast growth factor 2 (R&D Systems)) and passaged using Accutase (Innovative Cell Technologies)19 ...
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bioRxiv - Microbiology 2023Quote: ... and goat anti-human angiotensin converting enzyme 2 (ACE2; R&D Systems; 1:500). Appropriate species-specific secondary antibodies conjugated to AlexaFluor555 and/or AlexaFluor647 were applied for fluorescent detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antigen retrieval of ROR (R&D systems, N7927, Lot #A-2, 1:100), coronal 20 μm sections were boiled for 15 minutes in sodium citrate buffer (10mM sodium citrate ...
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bioRxiv - Immunology 2024Quote: ... goat anti-sex determining region Y-box 2 (1:200, R&D Systems, af2018); GFAP ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-SRY-box-containing gene 2 (SOX2, 1:250, AF2018, R&D Systems); rabbit anti-SRY-box-containing gene 2 (SOX2 ...
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bioRxiv - Developmental Biology 2024Quote: ... goat anti-SRY-box-containing gene 2 (SOX2, 1:250, AF2018, R&D Systems); rabbit anti-SRY-box-containing gene 2 (SOX2 ...
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bioRxiv - Immunology 2021Quote: ... After washing wells were incubated with a 5-bromo-4-chloro-3⍰-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (R&D systems, mouse IFNγ Kit Cat # EL485). Plates were scanned and analyzed using ImmunoSpot Microanalyzer.
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bioRxiv - Immunology 2021Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced after 48 hours (Day 2) with RPMI-1640 supplemented with B27 without insulin and 5 µM IWP2 (R&D Systems #3533). Media was again replaced after an additional 48 hours (Day 4 ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of stop solution (Sulfuric acid) (R&D systems) was added ...
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bioRxiv - Neuroscience 2022Quote: ... Pre warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and the absorbance was monitored at 450 nm with a Fluorostar galaxy plate reader.
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bioRxiv - Neuroscience 2022Quote: ... Pre-warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and absorbance at 450 nm was measured in a Fluorostar galaxy plate reader.
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bioRxiv - Bioengineering 2024Quote: ... hyaluronic acid (HA) (cat. #GLR001, R&D systems, Minneapolis, MN), ethanol (cat ...
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bioRxiv - Cancer Biology 2023Quote: The blots were probed overnight at 4°C with monoclonal mouse anti-β-Catenin (2 µg/mL in Bovine Serum Albumin 3%, R&D Systems, Minneapolis, USA, #MAB1329), monoclonal rabbit anti-NDRG4 (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Cancer Biology 2024Quote: ... Approximately 2 x 106 cells were injected in 100 µL of a 1:1 (v/v) mixture of Cultrex basement membrane extract (BME) type 3 (Biotechne R&D Systems, Minneapolis, MN, USA) and sterile 1X phosphate buffered saline (PBS) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... bEFNB2 expressing CHO cells were passaged in puromycin (1-5 ug/mL) for 1 week and then stained with EphB3-Fc (5667-B3-050; R&D Systems), a natural ligand of EFNB2/3 ...
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... caspase-3 (R&D Systems, AF835), Endomucin (Santa Cruz ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 µM CHIR (R&D Systems), 10 mM A83-01 (Tocris ...
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bioRxiv - Developmental Biology 2024Quote: ... SSEA-3 (R&D Systems, MAB1434) and Alexa Fluor 594 conjugated SSEA-4 (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were incubated at room temperature for 2 hours with primary antibodies (Megalin/LRP2, MyBiosource MBS690201, 1:500) (KIM-1, R&D Systems MAB1817, 1:250) in block solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA) or human IgG isotype control (#31154 ...
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bioRxiv - Microbiology 2024Quote: ... 1×105 spirochetes were pretreated with 0–5 μM hSLPI (R&D Systems, #1274-PI- 100) at 33°C for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 ng/ml recombinant human NRG1-β 1 (#396-HB, R&D Systems, USA) as described in Poitelon et al ...
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bioRxiv - Bioengineering 2021Quote: ... Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000, R&D Systems, Cat#MAB10474) was used for the detection of SARS-CoV-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 1:10 ratio and plated on 2-well comet slides (R&D Systems). Cells were then lysed overnight and immersed in alkaline unwinding solution as per manufacturer’s protocol (Trevigen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were incubated for 1 h with HRP-conjugated secondary antibodies diluted in 5% milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
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bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 5 days at 4°C with Car4 primary antibody (1:500, RRID:AB_10013483; R&D Systems), rinsed ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Cell Biology 2020Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.
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bioRxiv - Pathology 2022Quote: Serum KIM1 and NGAL concentrations were measured using mouse TIM-1/KIM-1/HAVCR and Lipocalin-2/NGAL quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Microbiology 2024Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Systems Biology 2024Quote: ... Intestinal and/or serum levels of LCN2 and IGF-1 were quantified using Mouse Lipocalin-2/NGAL DuoSet ELISA and Mouse/Rat IGF-I/IGF-1 DuoSet ELISA (R&D Systems), respectively ...
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bioRxiv - Microbiology 2021Quote: ... IFN-<ι>λ1/3 concentrations were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ng/mL mFGF2 (R&D Systems), 1xITS (Millipore Sigma)) ...
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bioRxiv - Bioengineering 2020Quote: ... cleaved caspase-3 (R&D System, MAB835) (1:150 in goat serum ...