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Citations for R&D Systems :
1901 - 1950 of 4052 citations for Rabbit Anti Human IgG Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated for 16 h prior to infection with addition of 50 IU mL-1 human IFNγ (285-IF, R&D Systems). Induction of GBP expression in the Dox-inducible cells was performed with 200 ng mL-1 Dox overnight (D9891 ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Neuroscience 2021Quote: ... Protein expression for the expressed human LTα transgene was measured in snap-frozen rat CSF using a human TNF-beta/TNFSF1 Quantikine ELISA kit (DTB00, R&D systems) as per manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Hippocampal homogenate samples were used to determine NRG1 levels with the Human NRG1-β1 ELISA (DY377, R&D Systems, Minneapolis MN, USA). Samples were diluted in kit assay buffer (1:6 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: Phosphorylation of several kinases and expression levels of matrix metallopeptidases (MMPs) were analyzed using Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) and Human MMP Antibody Array Kit (Abcam) ...
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bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: Cells were stimulated for 16 h prior to infection in complete medium at 37°C with addition of 50 IU/mL human IFNγ (285-IF, R&D Systems). Induction of Doxycycline-inducible cells was performed with 200 ng/mL Doxycycline overnight (D9891 ...
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bioRxiv - Systems Biology 2021Quote: ... washed once with FACS buffer (1%BSA in PBS and 5mM EDTA) and incubated for 40 minutes in FACS buffer containing 0.75ug of human ACE2-488 conjugated antibody (R&D Systems FAB9332) and 1:1000 7-AAD (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The secretome for each specimen was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (Ary007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
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bioRxiv - Cell Biology 2020Quote: Relative phosphorylation levels of 43 kinases and 2 related proteins were assessed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... conditioned medium was collected and the levels of secreted human CXCL8 and murine CXCL1 were detected with DuoSet ELISA (R&D systems) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Immunology 2022Quote: ... Calprotectin levels were determined in optimally diluted (1:20) extracts using Human-S100A8/S100A9 Heterodimer DuoSet-ELISA kit from R&D Systems. Zonulin levels were determined in similarly diluted extracts using human Zonulin Stool ELISA kit from ALPCO ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of IL11 in equal volumes of cell culture media were quantified using Human IL11 Quantikine ELISA kit (D1100; R&D Systems). Both assays were performed according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...
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bioRxiv - Bioengineering 2022Quote: Cytokines were measured in the supernatants from ASC cultures and ASC-enriched patches using the human cytokine base kit A (R&D Systems) combined with a magnetic Luminex assay (Bio-plex 200 ...
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bioRxiv - Immunology 2022Quote: ... cells were harvested and transferred to a 24 well plate and cultured in the presence of human rIL-2 (10U/ml, R&D Systems) for 4 additional days ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were immobilized on CM5-Sepharose chips coated by standard amine coupling chemistry to a density of about 600 response units (RU) Measurements were carried out at 25°C by injecting 250 μl of recombinant human TNFa (R&D Systems) at concentrations from 0.2 to 50 nM with a flow rate of 35μl/min ...
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bioRxiv - Immunology 2020Quote: Levels of human IL-1β (DY201) and IL-18 (DY318) were measured in the cell supernatants using ELISA kits from R&D Systems. Human IL-6 (# 88-7066-86 ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Microbiology 2021Quote: ... Barrier integrity was further assessed using the Human E-cadherin Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN). Absorbance was measured at 450 nm using a BioTek Synergy H2 plate reader and concentrations of E-cadherin were determined against a standard curve according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Cell Biology 2022Quote: The concentration of active TGF-β1 was measured using the Human /Mouse /Rat /Porcine /Canine TGF-beta 1 Quantikine ELISA kit (DB100B, R&D Systems). Briefly ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coated with a recombinant form of the human P-selectin ectodomain (spanning the 42-771 sequence of the preprotein) commercially available from R&D Systems by adaptation of a published protocol (Bally et al. ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked with a 10% Bovine Serum Albumin solution in PBS for 20 minutes and then incubated with the Alexafluor 647-conjugated antibody for Human ACE-2 (R&D Systems). After several washes ...
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bioRxiv - Cancer Biology 2022Quote: Changes in kinase phosphorylation upon exposure to the SASP was assayed using the Proteome Profiler Human Phospho-Kinase Array (R&D Systems). Briefly ...
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bioRxiv - Systems Biology 2019Quote: ... five µg of glycopeptide in 50 mM Tris-HCl pH 7.4 was mixed with one µg recombinant human C1GalT1/C1GalT1C1 protein (R&D Systems, NM) in the presence of either 0.5 mM UDP-Gal (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems). After 3 days of ActA treatment EBs were plated on fibronectin (20 μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... The supernatants from the serovar D infections were subjected to multiplex ELISA analyses in triplicate setting using a custom designed 27-plex human magnetic Luminex assay #LXSAHM (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total CREB and pCREB were measured using the Human/Mouse/Rat Phospho-CREB (S133) Cell-based ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Site specific phosphorylation levels of 43 kinases were measured using the Human Phospho-Kinase Array Kit (catalog no. ARY 003B, R&D Systems) according to the manufacturer’s instructions with the experimental design as described below ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteome Profiler Human Apoptosis Array kit (CAT# ARY009) and Pepstatin A aspartic protease inhibitor (#1190/10) were purchased from R&D Systems. The cOmplete protease inhibitor cocktail (CAT# 11697498001 ...
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bioRxiv - Cell Biology 2021Quote: Isolated human platelets were incubated at room temperature for 15-20 minutes with either recombinant HMGB1 (0-40 μg/ml; R&D Systems), ADP (0-5 μM ...
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bioRxiv - Cell Biology 2020Quote: ... For TGF-β1 detection in condition media from HPC treated with different experimental conditions and in urine samples from mice and human were analyzed by TGF-β1 ELISA commercial kit (R&D Systems) according to the man-ufacturer’s protocol.
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... The following antibodies were purchased and used for immunostaining of human sample using staining protocols similar to those used in mouse sections: CD31 (AF806, R&D systems), CXCR4 (2B11 ...
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bioRxiv - Developmental Biology 2019Quote: ... Human iPSC generated in this study were characterized for their differentiation potential into the three germ layers using Human Three Germ Layer Kit (R&D systems),according to the manufactures’ instructions with minor modifications ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Cell Biology 2019Quote: ... Cxcl10 and Ccl22 in mouse peritoneal cells were analyzed by ELISA according to the manufacturers instructions (Human and Mouse DuoSet ELISA respectively, R&D Systems). For relative chemokine concentration in comparison to parasite load ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...
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bioRxiv - Biochemistry 2019Quote: The corneal micropocket assay was performed as described16 using pellets containing 80 ng of basic fibroblast growth factor (bFGF) or 180 ng of human carrier-free recombinant vascular endothelial growth factor (VEGF; R&D Systems) in C57BL/6J mice ...
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bioRxiv - Cell Biology 2020Quote: ... and chondrogenic differentiation experiments were performed following the instructions of the human mesenchymal stem cell functional identification kit (R&D systems, SC006). For osteogenic differentiation ...
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bioRxiv - Microbiology 2021Quote: ... were isolated from whole blood (Washington National Primate Research Center) from two independent donors using human erythrocyte lysing kit (R&D Systems) and 95% Lymphoprep (Stemcell Technologies ...